Plant Soil Environ., 2026, 72(8):503-513 | DOI: 10.17221/260/2026-PSE

Radish growth and soil aggregate stability affected by microplastic contaminationOriginal Paper

Peter İurda ORCID...1, Lucia Toková ORCID...1, Lenka Botyanszká ORCID...1, Natália Botková ORCID...1
Institute of Hydrology, Slovak Academy of Sciences, Bratislava, Slovak Republic

The major objective of this study is to investigate changes in radish growth parameters (fresh and dry biomass and photosynthetic efficiency) and the stability of water-stable soil aggregates under contamination by different microplastic materials (high-density polyethylene (HDPE), polyvinyl chloride (PVC), and polystyrene (PS)) in contrasting soil types (Chernozem, Luvisol and Umbrisol). The study was conducted as a pot experiment under laboratory conditions, including three soil types and three microplastic types, with five replicates per variant. The results indicate that the effects of microplastics on plant biomass were significantly dependent on the interaction between soil type and microplastic type. In Chernozem, PS contamination significantly increased both dry and fresh biomass production (by approximately 46% and 50%, respectively) compared with the control. This response was directly associated with increased photosynthetic efficiency and greater stability of water-stable microaggregates in this variant. These findings confirm the stimulatory effect of PS on plant growth in Chernozem. The presence of PS in Chernozem appears to promote the formation of stable microaggregates and decrease macroaggregates, thereby altering the pore system (distribution of micro- and macropores) and supporting plant growth and nutrient absorption. An improvement in the stability of water-stable microaggregates was also observed in Luvisol and Umbrisol under PS contamination (statistically significant in Umbrisol); however, these changes were not reflected in the analysed plant growth parameters. In contrast, HDPE and PVC microplastic pollution had no statistically significant effect on biomass production or photosynthetic efficiency in Chernozem, Luvisol, or Umbrisol. Overall, the findings demonstrate that the effects of microplastic contamination on soil properties and plant growth are strongly influenced by both the type of microplastic polymer and soil characteristics.

Keywords: environmental contamination; pollutant; micro-particle effects; crop performance

Received: June 2, 2026; Revised: July 14, 2026; Accepted: August 5, 2026; Prepublished online: August 28, 2026; Published: August 29, 2026  Show citation

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İurda P, Toková L, Botyanszká L, Botková N. Radish growth and soil aggregate stability affected by microplastic contamination. Plant, Soil and Environment. 2026;72(8):503-513. doi: 10.17221/260/2026-PSE.
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    References

    1. Bachmann J., Horton R., van der Ploeg R.R., Woche S. (2000): Modified sessile drop method for assessing initial soil-water contact angle of sandy soil. Soil Science Society of America Journal, 64: 564-567. Go to original source...
    2. Boots B., Russell C.W., Green D.S. (2019): Effects of microplastics in ecosystems: above and below ground. Environmental Science and Technology, 53: 11496-11506. Go to original source... Go to PubMed...
    3. Colzi I., Renna L., Bianchi E., Castellani M.B., Coppi A., Pignattelli S., Loppi S., Gonnelli C. (2022): Impact of microplastics on growth, photosynthesis and essential elements in Cucurbita pepo L. Journal of Hazardous Materials, 423: 127238. Go to original source... Go to PubMed...
    4. Cottom J.W., Cook E., Velis C.A. (2024): A local-to-global emissions inventory of macroplastic pollution. Nature, 633: 101-108. Go to original source...
    5. De Souza Machado A.A., Lau C.W., Kloas W., Bergmann J., Bachelier J.B., Faltin E., Becker R., Görlich A.S. (2019): Microplastics can change soil properties and affect plant performance. Environmental Science Technology, 53: 6044-6052. Go to original source... Go to PubMed...
    6. Gao M., Liu Y., Song Z. (2019): Effects of polyethylene microplastic on the phytotoxicity of di-n-butyl phthalate in lettuce (Lactuca sativa L. var. ramosa Hort). Chemosphere, 237: 124482. Go to original source...
    7. Gong W., Zhang W., Jiang M., Li S., Liang G., Bu Q., Xu L., Zhu H., Lu A. (2021): Species-dependent response of food crops to poly-styrene nanoplastics and microplastics. The Science of the Total Environment, 796: 148750. Go to original source...
    8. Hanif M.N., Aijaz N., Azam K., Akhtar M., Laftah W.A., Babur M., Abbood N.K., Benitez I.B. (2024): Impact of microplastics on soil (physical and chemical) properties, soil biological properties/soil biota, and response of plants to it: a review. International Journal of Environmental Science and Technology, 21: 10277-10318. Go to original source...
    9. Hartmann N.B., Hüffer T., Thompson R.C., Hassellöv M., Verschoor A., Daugaard A.E., Rist S., Karlsson T., Brennholt N., Cole M., Herrling M.P., Hess M.C., Ivleva N.P., Lusher A.L., Wagner M. (2019): Are we speaking the same language? Recommendations for a definition and categorisation framework for plastic debris. Environmental Science and Technology, 53: 1039-1047. Go to original source... Go to PubMed...
    10. Jin T., Tang J., Lyu H., Wang L., Gillmore A.B., Schaeffer S.M. (2022): Activities of microplastics (MPs) in agricultural soil: a review of MPs pollution from the perspective of agricultural ecosystems. Journal of Agricultural and Food Chemistry, 70: 4182-4201. Go to original source... Go to PubMed...
    11. Joos L., De Tender C. (2022): Soil under stress: the importance of soil life and how it is influenced by (micro) plastic pollution. Com-putational and Structural Biotechnology Journal, 20: 1554-1566. Go to original source... Go to PubMed...
    12. Krehl A., Schöllkopf U., Májeková M., Tielbörger K., Tomiolo S. (2022): Effects of plastic fragments on plant performance are mediat-ed by soil properties and drought. Scientific Reports, 12: 17771. Go to original source...
    13. Lehner R., Weder C., Petri-Fink A., Rothen-Rutishauser B. (2019): Emergence of nanoplastic in the environment and possible impact on human health. Environmental Science and Technology, 53: 1748-1765. Go to original source... Go to PubMed...
    14. Li Z., Yang Y., Chen X., He Y., Bolan N., Rinklebe J., Lam S.S., Peng W., Sonne C. (2022): A discussion of microplastics in soil and risks for ecosystems and food chains. Chemosphere, 313: 137637. Go to original source...
    15. Li H., Liu G., Dan C., Zhou Z., Liu Y., Liu C., Shen E., Zhang Q., Xia X., Liu D., Guo Z., Zhao X. (2025): Effects of soil porosity on water stability of aggregates. Soil and Tillage Research, 254: 106741. Go to original source...
    16. Liu S., Suo Y., Wang J., Chen B., Wang K., Yang X., Zhu Y., Zhang J., Lu M., Liu Y. (2025): Impact of polystyrene microplastics on soil properties, microbial diversity and Solanum lycopersicum L. growth in meadow soils. Plants, 14: 256. Go to original source... Go to PubMed...
    17. Parkhill J.P., Maillet G., Cullen J.J. (2001): Fluorescence-based maximal quantum yield for PSII as a diagnostic of nutrient stress. Jour-nal of Phycology, 37: 517-529. Go to original source...
    18. Ragusa A., Svelato A., Santacroce C., Catalano P., Notarstefano V., Carnevali O., Papa F., Rongioletti M.C.A., Baiocoo F., Draghi S., D'Amore E., Rinaldo D., Matta M., Giorgini E. (2021): Plasticenta: first evidence of microplastics in human placenta. Environment International, 146: 106274. Go to original source... Go to PubMed...
    19. Ren F., Huang J., Yang Y. (2024): Unveiling the impact of microplastics and nanoplastics on vascular plants: a cellular metabolomic and transcriptomic review. Ecotoxicology and Environmental Safety, 279: 116490. Go to original source... Go to PubMed...
    20. Riaz K., Yasmeen T., Attia K.A., Kimiko I., Arif M.S. (2025): Phytotoxic effects of polystyrene microplastics on growth morphology, photosynthesis, gaseous exchange and oxidative stress of wheat vary with concentration and shape. Toxics, 13: 57. Go to original source... Go to PubMed...
    21. Shi X., Shi R., Fu X., Zhao Y., Ge Y., Liu J., Chen C., Liu W. (2024): Impact of microplastics on plant physiology: a meta-analysis of dose, particle size, and crop type interactions in agricultural ecosystems. Science of the Total Environment, 955: 177245. Go to original source... Go to PubMed...
    22. Stagnari F., Galieni A., D'Egidio S., Pagnani G., Pisante M. (2018): Responses of radish (Raphanus sativus) to drought stress. Annals of Applied Biology, 172: 170-186. Go to original source...
    23. İimanskŭ V., Wójcik-Gront E., Buchkina N., Horák J. (2023): Managing soil organic matter through biochar application and varying levels of N fertilisation increases the rate of water-stable aggregates formation. Journal of Hydrology and Hydromechanics, 71: 199-209. Go to original source...
    24. WRB (2015): Food and Agriculture Organisation of the United Nations (2015): World Reference Base for Soil Resources 2014. Inter-national Soil Classification System for Naming Soils and Creating Legends for Soil Maps. World Soil Resources Reports No. 106. Rome, FAO. Available at: FAO Open Knowledge Repository (accessed 11. 03. 2026).
    25. Wu X., Lu J., Du M., Xu X., Beiyuan J., Sarkar B., Bolan N., Xu S., Chen X., Wu F., Wang H. (2021): Particulate plastics-plant inter-action in soil and its implications: a review. Science of the Total Environment, 792: 148337. Go to original source... Go to PubMed...
    26. Yu Y.X., Battu A.K., Varga T., Denny A.C., Zahid T.M.D., Chowdhury I., Flury M. (2023): Minimal impacts of microplastics on soil physical properties under environmentally relevant concentrations. Environmental Science and Technology, 57: 5296-5304. Go to original source... Go to PubMed...
    27. Zang H., Zhou J., Marshall M.R., Chadwick D.R., Wen Y., Jones D.L. (2020): Microplastics in the agroecosystem: are they an emerging threat to the plant-soil system? Soil Biology and Biochemistry, 148: 107926. Go to original source...
    28. Zhao Z.Y., Wang P.Y., Wang Y.B., Zhou R., Kiprotich K., Ndolo M.A., Liu S.T., Wang W., Sun Y.Z., Xiong Y.C. (2021): Fate of plastic film residues in agroecosystem and its effects on aggregate-associated soil carbon and nitrogen stocks. Journal of Hazardous Materials, 416: 125954. Go to original source... Go to PubMed...

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