Plant Soil Environ., 2002, 48(7):318-321 | DOI: 10.17221/4367-PSE

Identification of sex in F1 progenies of hop (Humulus lupulus L.) by molecular marker

J. Patzak, P. Vejl, S. Skupinová, V. Nesvadba
1 Hop Research Institute Co., Ltd., Žatec, Czech Republic
2 Czech University of Agriculture in Prague, Czech Republic

Sex identification for hop (Humulus lupulus L.), which is a dioecious plant, is very important for breeding process. The use of molecular methods for marker-assisted selection (MAS) provides rapid and reliable identification of sex in F1 progenies. In the first, we proved the use of specific PCR molecular marker for evaluation of sex in selected plants. This marker was not amplified in 3 from 35 male plants. In the next, we successfully analysed 770 genotypes of F1 progenies of three crossings. The amplified differences were found in 4 male plants of crossing Sm01 H28, 8 male plants of crossing Sm01 H29 and 5 female plants of crossing Sm00 H20. Statistic analysis confirmed that sex ratios of all F1 progenies were significantly coincident. Obtained results confirmed that this specific PCR marker successfully identified sex of juvenile hop plants in F1 progenies and effectively improved breeding process.

Keywords: PCR; marker-assisted selection (MAS)

Published: July 31, 2002  Show citation

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Patzak J, Vejl P, Skupinová S, Nesvadba V. Identification of sex in F1 progenies of hop (Humulus lupulus L.) by molecular marker. Plant Soil Environ. 2002;48(7):318-321. doi: 10.17221/4367-PSE.
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