Plant Soil Environ., 2002, 48(8):356-360 | DOI: 10.17221/4380-PSE

Nodule efficiency of three soybean genotypes inoculated by different methods

V. Milić, N. Mrkovački, M. Popović, Đ. Malenčić
1 Institute of Field and Vegetable Crops, Novi Sad, Yugoslavia
2 Faculty of Agriculture, University of Novi Sad, Yugoslavia

The objective of the study was to investigate how the inoculation of soybean seed (variety Afrodita, and lines NS-L-2016 and NS-L-300168) with strains of Bradyrhizobium japonicum (1, 1a, 2b), Azotobacter chroococcum (3, 13, 14), and GA3 (gibberellic acid) affected plant dry weight, nitrogen content of nodules and whole plant, the enzymes of nitrogen assimilation (NR, GS) and soluble protein content. The highest dry matter mass and nitrogen content were found in the variety Afrodita, followed by line NS-L-300168. The GS and NR activity was increased significantly by all three inoculation treatments relative to the control. In all three genotypes, the highest values for the enzymatic activity were achieved with treatment mixture of B. japonicum and A. chroococcum strains. Each measurement was performed with three replications. The results were processed using variance analysis and the values were tested with the LSD at 5%.

Keywords: soybean; inoculation; nitrate reductase (NR); glutamine synthetase (GS); soluble protein content

Published: August 31, 2002  Show citation

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Milić V, Mrkovački N, Popović M, Malenčić Đ. Nodule efficiency of three soybean genotypes inoculated by different methods. Plant Soil Environ. 2002;48(8):356-360. doi: 10.17221/4380-PSE.
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