Plant Soil Environ., 2006, 52(10):454-458 | DOI: 10.17221/3466-PSE

Silybum marianum in vitro-flavonolignan production

L. Tůmová1, J. Řimáková1, J. Tůma2, J. Dušek1
1 School of Pharmacy, Charles University, Hradec Kralove, Czech Republic
2 Faculty of Education, University of Hradec Kralove, Czech Republic

The effect of coniferyl alcohol as a precursor of flavonolignan biosynthesis on silymarin components production in Silybum marianum suspension culture was studied. Coniferyl alcohol showed the changes in silymarin complex production. Silydianin was detected mainly in the control samples of cultivated cells. A significant increase of silydianin was observed only after 72 h of the application of 46µM coniferyl alcohol. No other components of the silymarin complex (silychristin and silybin) were detected; neither in control samples nor after the precursor feeding. But the increased accumulation of taxifolin (flavanole) was very interesting. The highest taxifolin production was reached after 48 hours of treatment(about 554% compared to control).

Keywords: milk thistle; Silybum marianum; in vitro culture; flavonolignans; coniferyl alcohol

Published: October 31, 2006  Show citation

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Tůmová L, Řimáková J, Tůma J, Dušek J. Silybum marianum in vitro-flavonolignan production. Plant Soil Environ. 2006;52(10):454-458. doi: 10.17221/3466-PSE.
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