Plant Soil Environ., 2007, 53(4):167-176 | DOI: 10.17221/2312-PSE

Comparison of the effectiveness of wheat roots colonization by Azotobacter chroococcum and Pantoea agglomerans using serological techniques

N. Narula2, R. Remus1, A. Deubel1, A. Granse2, S.S. Dudeja3, R.K. Behl1, W. Merbach2
1 Institutefor Soil Science and Plant Nutrition, Faculty of Agriculture, Martin-Luther University, Halle-Wittenberg, Germany
2 Department of Microbiology, CCS Haryana Agricultural University, Hisar, India
3 Department of Plant Breeding, CCS Haryana Agricultural University, Hisar, India

Colonization of Azotobacter chroococcum Mac 27 strain on wheat roots was monitored using double antibody sandwich enzyme linked immunosorbent assay (DAS ELISA) and immuno-fluorescence in terms of colonization sites, migration and survival of the bacteria. Furthermore, the effectivity of inoculation of A. chroococcum and Pantoea agglomerans D5/23 strain on wheat plant parameters under greenhouse conditions was investigated. Studies with DAS ELISA revealed that root tip had significant titre of inoculants as compared to the basal root parts. A. chroococcum colonized roots as well as soil and also migrated along with roots. An overall increase in plant growth was observed with inoculation of these bacteria. Both A. chroococcum and P. agglomerans were found to increase plant dry matter, nitrogen and phosphate uptake.

Keywords: Azotobacter chroococcum; Pantoea agglomerans; root colonization; serological testing; wheat; N and P uptake

Published: April 30, 2007  Show citation

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Narula N, Remus R, Deubel A, Granse A, Dudeja SS, Behl RK, Merbach W. Comparison of the effectiveness of wheat roots colonization by Azotobacter chroococcum and Pantoea agglomerans using serological techniques. Plant Soil Environ. 2007;53(4):167-176. doi: 10.17221/2312-PSE.
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    References

    1. Bahme J.B., Schroth M.N. (1987): Spatial-temporal colonization pattern of a rhizobacterium on underground organs of potato. Phytopathology, 77: 1093-1100. Go to original source...
    2. Baldani J.I., Caruso I., Baldani L.D., Goi S.R., Dobereiner J. (1997): Recent advances in BNF with nonlegume plants. Soil Biol. Biochem., 29: 911-922. Go to original source...
    3. Bashan Y. (1986): Significance of timing and level of inoculation with Rhizosphere bacteria on wheat plants. Soil Biol. Biochem., 18: 297-301. Go to original source...
    4. Behl R.K., Sharma H., Kumar V., Narula N. (2003): Interaction between mycorrhiza, Azotobacter chroococcum and root characteristics of wheat varieties. J. Agr. Crop Sci., 89: 151-155. Go to original source...
    5. Boddey R.M., Dobereiner J. (1988): Nitrogen fixation association with grasses and cereals: recent results and perspectives for future research. Plant Soil, 108: 653-655. Go to original source...
    6. Christiansen-Weniger C., Groneman A.F., Van Veen J.A. (1992): Associative N 2 fixation and root exudation of organic acids from wheat cultivars of different aluminium tolerance. Plant Soil, 139: 167-174. Go to original source...
    7. Clark F.M., Adams A.N. (1977): Characteristics of the micro plate method of enzyme linked immunosorbent assay for detection of plant viruses. J. Gen. Virol., 34: 475-483. Go to original source... Go to PubMed...
    8. Dobereiner J. (1995): Biological nitrogen fixation in tropics: Social and economic contributions. In: Proc. Int. Symp. Sustainable agriculture in tropics. The role of biological nitrogen fixation. Angra dos Reis: 3-4.
    9. Harris R.F., Sommers L.E. (1968): Plate dilution frequency technique for assay of microbial ecology. Appl. Microbiol., 16: 330-334. Go to original source... Go to PubMed...
    10. Hirte W.F. (1961): Glycerin-Pepton-Agar ein vorteilhafter Nährboden für Boden bakteriologische Arbeiten. Zbl. Bakt. Parasitenk. Infektionskrankh. Hyg., 114: 141-146.
    11. Höflich G., Wiehe W., Kühn G. (1994): Plant growth stimulation by inoculation with symbiotic and associative rhizosphere microorganisms. Experientia, 50: 897-905. Go to original source...
    12. Jiang H.Y., Sato K. (1994): Interrelationships between bacterial populations on the root surface of wheat and growth of plants. Soil Sci. Plant Nutr., 40: 683-689. Go to original source...
    13. Kloepper J.W., Beauchamp C.J. (1992): A review of issues related to measuring colonisation of plant roots by bacteria. Can. J. Microbiol., 38: 1219-1232. Go to original source...
    14. Kumar V., Narula N. (1999): Solubilization of inorganic phosphates by Azotobacter chroococcum mutants and their effect on seed emergence of wheat. Biol. Fertil. Soil, 28: 301-305. Go to original source...
    15. Kvalseth T.O. (1985): Cautionary note about R 2. Am. Stat., 39: 279-285. Go to original source...
    16. Lakshminarayana K. (1993): Influence of Azotobacter on nutrition of plant and crop productivity. In: Proc. Indian Nat. Sci. Acad., B59: 303-308.
    17. Lakshminarayana K., Narula N., Hooda I.S., Faroda A.S. (1992): Nitrogen economy in wheat (Triticum aestivum) through use of A. chroococcum. Indian J. Agr. Sci., 62: 75-76.
    18. Marschner P., Crowley D.E., Higashi R.M. (1997): Root exudation and physiological status of root colonizing fluorescent pseudomonad in mycorrhizal and nonmycorrhizal pepper (Capsicum annuum L.). Plant Soil, 189: 11-20. Go to original source...
    19. McCully J.S., Canny M.J. (1988): Pathways and processes of water and nutrient movement in roots. Plant Soil, 111: 159-170. Go to original source...
    20. Narula N., Deubel A., Gans W., Behl R.K., Merbach W. (2006): Paranodules and colonization of wheat roots by phytohormone producing bacteria in soil. Plant Soil Environ., 52: 119-129. Go to original source...
    21. Narula N., Kumar V., Behl R.K., Deubel A., Granse A., Merbach W. (2000): Effect of P-solubilizing A. chroococcum on NPK uptake in P responsive wheat genotypes grown under green house conditions. J. Plant Nutr. Soil Sci., 163: 93-398. Go to original source...
    22. Narula N., Nijhawan D.C., Lakshminarayana K., Kapoor R.L., Verma O.P.S. (1991): Response of pearl millet (Pennisetum glaucum) to soil isolates and analogue resistant mutants of A. chroococcum. Indian J. Agr. Sci., 61: 484-487.
    23. Ouchterlony O. (1962): Diffusion in gel methods for immunological analysis II. In: Kallos P., Waksman B.H. (eds.): Progress in Allergy. Vol. VI. Basel, Karger: 30-154. Go to original source...
    24. Pandey A., Kumar S. (1989): Potential of Azotobacter and Azospirillum as biofertilizers for upland agriculture. A review. J. Sci. Industr. Res., 48: 134-144.
    25. Parke J.L. (1991): Root colonization by indigenous and introduced microorganisms. In: Keister D.L., Cregan P.B. (eds.): The Rhizosphere and Plant Growth. Kluwer Acad. Publ., The Netherlands: 33-42. Go to original source...
    26. Pathak D.V., Lakshminarayana K., Narula N. (1995): Analogue resistant mutants of A. chroococcum affecting growth parameters in sunflower (Helianthus annuus L.) under pot culture conditions. Nat. Acad. Sci. Lett., 18: 203-203.
    27. Rattray E.A.S., Prosser J.I., Glover L.A., Killham K. (1995): Characterisation of rhizosphere colonization by luminescent Enterobacter cloacae at the population and single-cell levels. Appl. Environ. Microbiol., 61: 2950-2957. Go to original source... Go to PubMed...
    28. Remus R., Ruppel S., Jacob H.J., Hecht-Buchholz C., Merbach W. (2000): Colonization behaviour of two enterobacterial strains on cereals. Biol. Fertil. Soils, 30: 550-557. Go to original source...
    29. Ruppel S., Hecht-Buchholz C., Remus R., Ortmann U., Schmelzer R. (1992): Settlement of the diazotrophic, phytoeffective bacterial strain P. agglomerans on and within winter wheat: An investigation using ELISA and transmission microscopy. Plant Soil, 145: 261-273. Go to original source...
    30. Scholz C., Remus R., Zielke R. (1991): Development of DAS-ELISA for some selected bacteria from the rhizosphere. Zbl. Mikrobiol., 146: 197-207. Go to original source...
    31. Scholz-Seidel C., Ruppel S. (1992): Nitrogenase and phytohormone activities of Pantoea agglomerans in culture and their reflection in combination with wheat plants. Zbl. Mikrobiol., 147: 319-328. Go to original source...
    32. Singh R., Behl R.K., Singh K.P., Jain P., Narula N. (2004): Performance and gene effects for wheat yield under inoculation of arbuscular mycorrhiza fungi and Azotobacter chroococcum. Plant Soil Environ., 50: 409-415. Go to original source...
    33. Van Berkum P., Bohlool B.B. (1980): Evaluation of nitrogen fixation by bacteria in association with roots of tropical grasses. Microbiol. Rev., 44: 491-517. Go to original source... Go to PubMed...
    34. Verma S., Narula N., Kumar V., Merbach W. (2001): Studies on in vitro production of antimicrobial substances by Azotobacter chroococcum isolate/mutants. J. Plant Dis. Protec., 108: 152-162.
    35. Wani S.P., Chandrapalaiah S.S., Zambre S., Lee K.K. (1989): Association between N 2 fixing bacteria and pearl millet plants: Responses mechanisms and persistence. In: Skinner F.A., Boddey R.M., Fendrik I. (eds.): Nitrogen fixation with non-legumes. Kluwer Acad. Publ., Dordrecht, The Netherlands: 249-262. Go to original source...
    36. Weller D.M. (1988): Biological control of soil borne pathogens in the rhizosphere with bacteria. Ann. Rev. Phytopathol., 26: 379-407. Go to original source...
    37. Yadav K.S., Singh N., Suneja S., Malik Y.S., Nehra B.K., Narula N. (2003): Nitrogen economy through the use of biofertilizer in potato. J. Indian Potato Assoc., 30: 81-82.
    38. Zimmer W., Bothe H. (1988): The phytohormonal interactions between Azospirillum and wheat. Plant Soil, 110: 239-247. Go to original source...
    39. Zimmer W., Roeben K., Bothe H. (1988): An alternate explanation for plant growth promotion by bacteria of the genus Azospirillum. Planta, 176: 333-342. Go to original source... Go to PubMed...

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