Plant Soil Environ., 2007, 53(10):437-441 | DOI: 10.17221/2195-PSE

Cot-based cloning and sequencing of the short arm of wheat chromosome 1B

H. Šimková, J. Janda, E. Hřibová, J. Šafář, J. Doležel
Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Olomouc, Czech Republic

Sequencing of cereal genomes is not a feasible task due to their large size and high content of repetitive DNA sequences. There are two basic approaches to simplify analysis of such genomes: reduced representation approaches, such as EST sequencing, methyl filtration and Cot-based cloning and sequencing; on the other side there is analysis of genomes in a step-wise manner, e.g. through creation of chromosome-specific genomic resources. Combination of both approaches - i.e. Cot-based cloning and sequencing of DNA obtained from a chromosome-arm-specific BAC library - was tested in this work.

Keywords: Cot; CBCS; Cot filtration; BAC library; 1 BS; wheat; genome analysis

Published: October 31, 2007  Show citation

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Šimková H, Janda J, Hřibová E, Šafář J, Doležel J. Cot-based cloning and sequencing of the short arm of wheat chromosome 1B. Plant Soil Environ. 2007;53(10):437-441. doi: 10.17221/2195-PSE.
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