Plant Soil Environ., 2008, 54(11):498-508 | DOI: 10.17221/436-PSE

Rhizobia associated with neotropical tree Centrolobium tomentosum used in riparian restoration

M.C. Pagano
Department of Botany, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil

Centrolobium tomentosum is a tropical legume tree indicated for functional and structural restoration of riparian areas. This study was conducted to characterize the rhizobia isolated from nodules of C. tomentosum in situ and to determine their capacity of renodulation, in an experimental area of land rehabilitation in the Rio Doce valley. Nodulation potential to inoculation with 2 selected fast-growing Rhizobium strains separately and a mixed inoculum of arbuscular mycorrhizal fungi was evaluated by the use of antibiotics resistance. Flood disturbance were observed not to affect renodulation by fast-growing strains. DNA fingerprinting RAPD (random amplified polymorphic DNA) and lipopolysaccharides (LPS) profiles were used to examine molecular relationships among field isolates, inoculants and reference strains. Maximal renodulation was exhibited by strain BHCBAb1 after 24 months after transplantation. Centrolobium tomentosum forms symbiosis with fast- and slow-growing Rhizobium strains, and it is suggested that their nursery culture could be improved by inoculation of selected strain under low nitrogen-input conditions.

Keywords: Centrolobium tomentosum; tree legume; Rhizobium; inoculation; renodulation; Doce River

Published: November 30, 2008  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Pagano MC. Rhizobia associated with neotropical tree Centrolobium tomentosum used in riparian restoration. Plant Soil Environ. 2008;54(11):498-508. doi: 10.17221/436-PSE.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Chen W.X., Wang E., Wang S., Li Y., Chen X., Li Y. (1995): Characteristics of Rhizobium tianshanense sp. nov., a moderately and slowly growing root nodule bacterium isolated from an arid saline environment in Xinjiang, P.R. China. Int. J. Syst. Bacteriol., 45: 153-159. Go to original source... Go to PubMed...
    2. De Maagd R.A., Van Rossum C., Lugtenberg B.J.J. (1988): Recognition of individual strains of fast-growing rhizobia by using profiles of membrane proteins and lipopolysaccharides. J. Bacteriol., 170: 3782-3785. Go to original source... Go to PubMed...
    3. Dommergues Y.R., Diem H.G., Gauthier D.L., Dreyfus B.L., Cornet F. (1984): Nitrogen-fixing trees in the tropics: potentialities and limitations. In: Veeger C., Newton W.E. (eds.): Advances in Nitrogen Fixation Research. Martinus Nijhoff/Dr. W. Junk Publishers, Wageningen: 164-167. Go to original source...
    4. Dupuy N.C., Dreyfus B.L. (1992): Bradyrhizobium populations occur in deep soil under the leguminous tree Acacia albida. Appl. Environ. Microbiol., 58: 2415-2419. Go to original source... Go to PubMed...
    5. Heal O.W., Ineson P. (1984): Carbon and energy flow in terrestrial ecosystems: relevance to microflora. In: Klug M.J., Reddy C.A. (eds.): Current Perspectives in Microbial Ecology. American Society for Microbiology, Washington D.C.: 394-404.
    6. Herrera M.A., Salamanca C.P., Barea J.M. (1993): Inoculation of woody legumes with selected arbuscular mycorrhizal fungi and rhizobia to recover desertified Mediterranean ecosystems. Appl. Environ. Microbiol., 59: 129-133. Go to original source... Go to PubMed...
    7. Jenkins M.B., Virginia R.A., Jarrell W.M. (1987): Rhizobial ecology of the woody legume mesquite (Prosopis glandulosa) in the Sonoran Desert. Appl. Environ. Microbiol., 53: 36-40. Go to original source... Go to PubMed...
    8. Kremer R.J., Peterson H.L. (1982): Nodulation efficiency of legume inoculation as determined by intrinsic antibiotic resistance. Appl. Environ. Microbiol., 43: 636-642. Go to original source... Go to PubMed...
    9. Lal B., Khanna S. (1993): Renodulation and nitrogen fixing potential of Acacia nilotica inoculated with Rhizobium isolates. Can. J. Microbiol., 39: 87-91. Go to original source...
    10. Makatiani E., Odee D.W. (2007): Response of Sesbania sesban (L.) Merr. to rhizobial inoculation in an N-deficient soil containing low numbers of effective indigenous rhizobia. Agrofor. Syst., 70: 211-216. Go to original source...
    11. Manassila M., Nuntagij A., Kotepong S., Boonkerd N., Teaumroong N. (2007): Characterization and monitoring of selected rhizobial strains isolated from tree legumes in Thailand. Afr. J. Biotechnol., 6: 1393-1402.
    12. Marques M.S., Pagano M.C., Scotti M.R.M. (2001): Dual inoculation of woody legume (Centrolobium tomentosum) with rhizobia and mycorrhizal fungi in southeastern Brazil. Agrofor. Syst., 52: 107-117. Go to original source...
    13. Mnasri B., Mrabet M., Laguerre G., Elarbi M., Mhamdi A.R. (2007): Salt-tolerant rhizobia isolated from a Tunisian oasis that are highly effective for symbiotic N2-fixation with Phaseolus vulgaris constitute a novel biovar (bv. mediterranense) of Sinorhizobium meliloti. Arch. Microbiol., 187: 79-85. Go to original source... Go to PubMed...
    14. Moreira F.M.S., Gillis M., Pot B., Kersters K., Franco A.A. (1993): Characterization of rhizobia isolated from different divergence groups of tropical Leguminosae by comparative polyacrylamide gel electrophoresis of their total proteins. Syst. Appl. Microbiol., 16: 135-146. Go to original source...
    15. Odee D.W., Haukka K., McInroy S.G., Sprent J.I., Sutherland J.M., Young J.P.W. (2002): Genetic and symbiotic characterization of rhizobia isolated from tree and herbaceous legumes grown in soils from ecologically diverse sites in Kenya. Soil Biol. Biochem., 34: 801-811. Go to original source...
    16. Rao A.V., Tak R. (2001): Influence of mycorrhizal fungi on the growth of different tree species and their nutrient uptake in gypsum mine spoil in India. Appl. Soil Ecol., 17: 279-284. Go to original source...
    17. Sá N.M.H., Scotti M.R.M., Paiva E., Franco A.A., Döbereiner J. (1993): Selection and characterization of Rhizobium strains stable and efficient in the fixation of nitrogen in bean (Phaseolus vulgaris L.). Rev. Microbiol., 24: 38-48.
    18. Somasegaram P., Hoben H.J. (1985): Methods in Legume-Rhizobium Technology. NIFTAL, Hawai.
    19. Steindel M., Dias Neto E., Menezes C.L.P., Romanha A.J., Simpson A.J.G. (1993): Random amplified polymorphic DNA analysis of Trypanosoma cruzi strains. Mol. Biochem. Parasitol., 60: 71-80. Go to original source... Go to PubMed...
    20. Tas E., Leinonen P., Saano A., Rasanen L.A., Kaijalainen S., Piippola S., Hakola S., Lindstrom K. (1996): Assessment of competitiveness of rhizobia infecting Galega orientalis on the basis of plant yield, nodulation, and strain identification by antibiotic resistance and PCR. Appl. Environ. Microbiol., 65: 529-535. Go to original source... Go to PubMed...
    21. Tian C., He X., Zhong Y., Chen J. (2003): Effect of inoculation with ecto- and arbuscular mycorrhizae and Rhizobium on the growth and nitrogen fixation by black locust, Robinia pseudoacacia. New For., 25: 125-131. Go to original source...
    22. Vincent J.M. (1970): A Manual for the Practical Study of the Root Nodule Bacteria. Blackwell Scientific Publications, Oxford.
    23. Wang F.Q., Wang E.T., Zhang Y.F., Chen W.X. (2006): Characterization of rhizobia isolated from Albizia spp. in comparison with microsymbionts of Acacia spp. and Leucaena leucocephala grown in China. Syst. Appl. Microbiol., 29: 502-517. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content