Plant Soil Environ., 2009, 55(1):11-16 | DOI: 10.17221/326-PSE

The influence of fertilisation and crop rotation on soil microbial characteristics in the long-term field experiment

O. Mikanová, M. Friedlová, T. Šimon
Crop Research Institute, Prague-Ruzyně, Czech Republic

Soils were sampled from the plots with four variants of fertilisation: Nil - without fertilisation, NPK - mineral fertilisation, FYM - farmyard manure, FYM + NPK - farmyard manure with mineral fertilisation, and two variants of crop rotation: field IV - classical 9-year crop rotation, field B - 2-year rotation of alternative growing. Determination of urease, CFU of Azotobacter spp. and potential nitrogenase activity was conducted during the period 1999-2004. The urease activity was positively affected by manure fertilisation (FYM) and by the combination of FYM + NPK. The statistically significantly highest counts of Azotobacter spp. and the highest nitrogenase activity were determined on field B in variants FYM and FYM + NPK. The results show that there was a higher amount of accessible nitrogen present on field IV than on field B. This might explain the lower counts of Azotobacter spp. and therefore the lower nitrogenase activity. According to our results, activity of urease, CFU of Azotobacter spp. and potential nitrogenase activity are very closely connected with N inputs.

Keywords: urease; Azotobacter spp.; fertilisation; potential nitrogenase aktivity

Published: January 31, 2009  Show citation

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Mikanová O, Friedlová M, Šimon T. The influence of fertilisation and crop rotation on soil microbial characteristics in the long-term field experiment. Plant Soil Environ. 2009;55(1):11-16. doi: 10.17221/326-PSE.
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    References

    1. Balakrishnan V., Venkatesan K., Ravindran K.C. (2007): The influence of halophytic compost, farmyard manure and phosphobacteria on soil microflora and enzyme activities. Plant Soil Environ., 53: 186-192. Go to original source...
    2. Buresh R.J., Casselman M.E., Patrick Jr.W.H. (1980): Nitrogen fixation in flood soil systems, a review. Adv. Agron., 23: 149-192. Go to original source...
    3. Czakó A., Mikanová O., Usťak S. (2007): The effect of inoculation on reclaimed soils. Počvoved. Agrochim., 1: 232-237.
    4. Gianfreda L., Bollag J.M. (1996): Influence of natural and anthropogenic factors on enzyme activity in soil. Soil Biochem., 9: 123-193.
    5. Kandeler E., Gerber H. (1988): Short-term assay of soil urease activity using colorimetric determination of ammonium. Biol. Fertil. Soils, 6: 68-72. Go to original source...
    6. Kandeler E., Stemmer M., Klimanek E. (1999): Response of soil microbial biomass, urease and xylanase within particle size fractions to long-term soil management. Soil Biol. Biochem., 31: 261-273. Go to original source...
    7. Kennedy I.G., Choudhury M.A., Kecskés M.L. (2004): Non-symbiotic bacterial diazotrophs in crop-farming systems: can their potential for plant growth promotion be better exploited? Soil Biol. Biochem., 36: 1229-1244. Go to original source...
    8. Kubát J., Cerhanová D., Mikanová O., Hanzlíková A., Filip Z. (1996): Effects of long-term fertilisation and anthropogenic load on activity of the soil microflora. Rostl. Výr., 42: 399-404. (In Czech)
    9. Kubát J., Klír J., Apfelthaler R. (1997): Utilization of the long-term field experiment in Prague-Ruzyne in modern agricultural research. Arch. Acker-Pfl.-Bau Bodenkde, 42: 181-191. Go to original source...
    10. Kubát J., Klír J., Cerhanová D. (2001): Quantification of the carbon and nitrogen cycles in long-term field experiments in Prague. Arch. Acker-Pfl.-Bau Bodenkde, 46: 297-311. Go to original source...
    11. Kubát J., Nováková J., Cerhanová D., Apfelthaler R. (1999): Organic nitrogen cycle, ammonification and nitrification activity in long-term field experiment. Rostl. Výr., 45: 397-402.
    12. Mikanová O., Nováková J., Hanzlíková A., Kubát J., Filip Z. (1996): Effects of long-term fertilisation and anthropogenic load on biomass and the incidence of some groups of microorganisms. Rostl. Výr., 42: 391-397. (In Czech)
    13. Narula N., Remus R., Deubel A., Granse A., Dudeja S.S., Behl R.K., Merbach W. (2007): Comparison of the effectiveness of wheat roots colonization by Azotobacter chroococcum and Pantoea agglomerans using serological techniques. Plant Soil Environ., 53: 167-176. Go to original source...
    14. Nayak D.R., Babu Y.J., Adhya T.K. (2007): Long-term application of compost influences microbial biomass and enzyme activities in a tropical Aeric Endoaquept planted to rice under flooded condition. Soil Biol. Biochem., 39: 1897-1906. Go to original source...
    15. Ondrášek Ľ., Čunderlík J. (2008): Effects of organic and mineral fertilisers on biological properties of soil under seminatural grassland. Plant Soil Environ., 54: 329-335. Go to original source...
    16. Patra A.K., Le Roux X., Abbadie L., Clays-Josserand A., Poly F., Loiseau P., Louault F. (2007): Effect of microbial activity and nitrogen mineralization on free-living nitrogen fixation in permanent grassland soils. J. Agron. Crop Sci., 193: 153-156. Go to original source...
    17. Šimek M. (1993): Biological nitrogen fixation (nitrogenase activity) as a measure of soil biological activity. Rostl. Výr., 39: 789-796. (In Czech)
    18. Yoch D.C., Whiting G.J. (1986): Evidence for NH 4+ switch-off regulation of nitrogenase activity by bacteria in salt marsh sediments and roots of the grass Spartina alterniflora. Appl. Environ. Microbiol., 117: 53-60. Go to original source... Go to PubMed...

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