Plant Soil Environ., 2009, 55(6):238-244 | DOI: 10.17221/84/2009-PSE
Nitrogen compounds and enzyme activities in sorghum induced to water deficit during three stages
- 1 Laboratório de Fisiologia Vegetal Avançada, Universidade Federal Rural da Amazônia, Belém, Brazil
- 2 Núcleo de Pesquisa Aplicada a Agricultura, Universidade Estadual de Maringá,
The aim of this study was to evaluate and explicate the changes in the nitrogen compounds and enzyme activities in Sorghum bicolor plants (cv. BR-700) submitted to water deficit during vegetative, reproductive and maturation stages. The experimental design used was entirely randomized in factorial scheme, with two conditions (control and stress) combined with three stages (vegetative, reproductive and maturation). The nitrate level was increased at 180.6, 72.9 and 78.9% during the vegetative, reproductive and maturation stages, respectively. The total soluble amino acids and glycinebetaine increased only during vegetative and reproductive stages, if compared with control plants. However, besides protein levels during all stages, significant reductions were reported in nitrate reductase and glutamine synthetase activities; free ammonium presented decreases at 37.3 and 77.6% in vegetative and reproductive stages, respectively, when compared with control plants.
Keywords: Sorghum bicolor L.; nitrogen; nitrate; ammonium; water deficit
Published: June 30, 2009 Show citation
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References
- Agboma P., Jones M.G.K., Peltonen-Sainio P., Rita H., Pehu E. (1997): Exogenous glycinebetaine enhances grain yield of maize, sorghum and wheat grown under two supplementary watering regimes. Journal of Agronomy and Crop Science, 178: 29-37.
Go to original source...
- Bradford M.M. (1976): A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72: 248-254.
Go to original source...
- Cataldo D.A., Haroon S.L., Yougs V.L. (1975): Rapid colorimetric determination of nitrate in plant tissue by nitration of salicylic acid. Communications in Soil Science and Plant Analysis, 6: 71-80.
Go to original source...
- Chen T.H.H., Murata N. (2002): Enhancement of tolerance of abiotic stress by metabolic engineering of betaines and other compatible solutes. Current Opinion in Plant Biology, 5: 250-257.
Go to original source...
Go to PubMed...
- Costa R.C.L. (1999): Nitrogen assimilation and osmotic adjustment in nodulated cowpea Vigna unguiculata L. (Walp) plants submitted to water stress. [Ph.D. Thesis.] Universidade Federal do Ceará, Brasil.
- Debouba M., Gouia H., Suzuki A., Ghorbel M.H. (2006): NaCl stress effects on enzymes involved in nitrogen assimilation pathway in tomato Lycopersicon esculentum seedlings. Journal of Plant Physiology, 163: 1247-1258.
Go to original source...
Go to PubMed...
- Grieve C.M., Grattan S.R. (1983): Rapid assay for determination of water soluble quaternary ammonium compounds. Plant and Soil, 70: 303-307.
Go to original source...
- Hageman R.H.G., Hucklesby D.P. (1971): Nitrate reductase from higher plants. Methods in Enzymology, 17: 491-503.
Go to original source...
- Hoagland D.R., Arnon D.I. (1950): The water culture method for growing plants without soil. California Agricultural Experiment Station, Circular, 347.
- Kamachi K., Yamaya T., Mae T., Ojima K. (1991): A role for glutamine synthetase in remobilization of leaf nitrogen during natural senescence in rice leaves. Plant Physiology, 96: 411-417.
Go to original source...
Go to PubMed...
- Kerbauy G.B. (2004): Plant Physiology. Guanabara Koogan S.A., Rio de Janeiro.
- Lobato A.K.S., Meirelles A.C.S., Santos Filho B.G., Costa R.C.L., Oliveira Neto C.F., Cruz F.J.R., Freitas J.M.N., Guedes E.M.S., Barreto A.G.T., Ferreira A.S., Monteiro B.S., Neves H.K.B., Lopes M.J.S. (2008a): Consequences of the progressive water deficit and rehydration on reductase activity and nitrogen compounds in soybean (Glycine max cv. Sambaiba). Research Journal of Agronomy, 2: 64-70.
- Lobato A.K.S., Oliveira Neto C.F., Costa R.C.L., Santos Filho B.G., Silva F.K.S., Cruz F.J.R., Abboud A.C.S., Laughinghouse IV H.D. (2008b): Germination of sorghum under the influences of water restriction and temperature. Journal of Agricultural, 3: 220-224.
- Lobato A.K.S., Oliveira Neto C.F., Santos Filho B.G., Cruz F.J.R., Neves H.K.B., Lopes M.J.S. (2008c): Physiological and biochemical behavior in soybean (Glycine max cv. Sambaiba) plants under water deficit. Australian Journal of Crop Science, 2: 25-32.
- Lobato A.K.S., Costa R.C.L., Oliveira Neto C.F., Santos Filho B.G., Gonçalves-Vidigal M.C., Vidigal Filho P.S., Silva C.R., Cruz F.J.R., Camargo P.M.P., Santos P.C.M. (2009): Consequences of the water deficit on water relations and symbiosis in Vigna unguiculata cultivars. Plant, Soil and Environment, 55: 139-145.
Go to original source...
- Oliveira J.S., Ferreira R.P., Cruz C.D., Pereira A.V., Botrel M.A., Von Pinho R.G., Rodrigues J.A.S., Lopes F.C.F., Miranda J.E.C. (2002): Adaptability and stability in sorghum cultivars. Revista Brasileira de Zootecnia, 31: 883-889.
Go to original source...
- Patel A.D., Pandey A.N. (2008): Growth, water status and nutrient accumulation of seedlings of Holoptelea integrifolia (Roxb.) Planch in response to soil salinity. Plant, Soil and Environment, 54: 367-373.
Go to original source...
- Peoples M.B., Faizah A.W., Reakasem B.E, Herridge D.F. (1989): Methods for Evaluating Nitrogen Fixation by Nodulated Legumes in the Field. Australian Centre for International Agricultural Research, Canberra.
- Pimentel C. (2004): The Relationship of the Plant with the Water. EDUR, Seropédica, New York.
- Plhák F. (2003): Nitrogen supply through transpiration mass flow can limit nitrogen nutrition of plants. Plant, Soil and Environment, 49: 473-479.
Go to original source...
- Slavick B. (1979): Methods of Studying Plant Water Relations. Springer-Verlag, New York.
- Silva S., Soares M., Oliveira L.E.M. (2001): Physiologic responses of important grasses to gallery forest revegetation of the hydroelectric reservatories, submitted to water deficit. Ciência e Agrotecnologia, 25: 124-133.
- Weatherburn M.W. (1967): Phenol hypochlorite reaction for determination of ammonia. Analytical Chemistry, 39: 971-974.
Go to original source...
- Xiong L., Scumaker K.S., Zhu J.K. (2002): Cell signaling during cold, drought and stress. The Plant Cell, 14: 165-183.
Go to original source...
Go to PubMed...
- Yadav S.K., Jyothi Lakshmi N., Maheswari M., Vanaja M., Venkateswarlu B. (2005): Influence of water deficit at vegetative, anthesis and grain filling stages on water relation and grain yield in sorghum. Indian Journal of Plant Physiology, 10: 20-24.
- Zhu J., Hasegawa P.M., Bressan R.A. (1997): Molecular aspects of osmotic stress in plants. Critical Review of Plant Science, 16: 253-277.
Go to original source...
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