Plant Soil Environ., 2009, 55(9):397-403 | DOI: 10.17221/1012-PSE

Modification of the terminal restriction fragment length polymorphism analysisfor assessment of a specific taxonomic group within a soil microbial community

J. Kopecký, G. Novotná, M. Ságová-Marečková
Crop Research Institute, Prague, Czech Republic

A double color terminal restriction fragment length polymorphism analysis was applied for the diversity assessment of a complex bacterial community in a deciduous forest soil. Application of the second label was used to project a specific taxonomic group of bacteria, actinomycetes in our case, to the universal fingerprint. The diversity of actinomycetes was evaluated directly as a percentage of the whole bacterial community by comparing the corresponding terminal fragments of the same length.

Keywords: bacterial community; Actinobacteria; terminal restriction fragment length polymorphism

Published: September 30, 2009  Show citation

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Kopecký J, Novotná G, Ságová-Marečková M. Modification of the terminal restriction fragment length polymorphism analysisfor assessment of a specific taxonomic group within a soil microbial community. Plant Soil Environ. 2009;55(9):397-403. doi: 10.17221/1012-PSE.
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