Plant Soil Environ., 2010, 56(5):201-208 | DOI: 10.17221/231/2009-PSE

Nitric oxide emission from barley seedlings and detached leaves and roots treated with nitrate and nitrite

J. Chen1,2, Q. Xiao2,3, F.H. Wu1,2, Z.M. Pei4,2, J. Wang5, Y.G. Wu5, H.L. Zheng1,2,6
1 Key Laboratory for Subtropical Wetland Ecosystem Research, Ministry of Education of China, Xiamen University, Xiamen, P.R. China
2 School of Life Sciences, Xiamen University, Xiamen, P.R. China
3 Laboratory of Biological Resources Protection and Utilization of Hubei Province, Hubei Institutes for Nationalities, Enshi, P.R. China
4 Department of Biology, Duke University, Durham, USA
5 Center for Environmental Monitoring, Xiamen Environmental Protection Agency, Xiamen, P.R. China
6 State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, P.R. China

Nitric oxide (NO) emission from detached barley leaves, roots and whole plants treated with various nitrate or nitrite concentrations under light/dark and aerobic/anaerobic conditions was quantified by using a chemiluminescence detector. NO emission from detached tissues and whole plants treated with moderate nitrate concentration (60mM) was relatively higher under anaerobic condition, and was positively correlated with nitrite concentration. Darkness and anaerobic condition remarkably induced NO emission from detached barley leaves. On the contrary, NO emission from detached roots and whole plants was relatively higher in light. As for whole plants treated with 60mM nitrate and 12mM nitrite, the pattern of NO emission in normal environment was broken by light-dark and aerobic-anaerobic transition. Light and anaerobic condition induced NO emission significantly in the whole plant. The whole barley plant emitted significantly higher amount of NO than detached leaves or roots.

Keywords: Hordeum vulgare; chemiluminescence; light; dark; aerobic; anaerobic

Published: May 31, 2010  Show citation

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Chen J, Xiao Q, Wu FH, Pei ZM, Wang J, Wu YG, Zheng HL. Nitric oxide emission from barley seedlings and detached leaves and roots treated with nitrate and nitrite. Plant Soil Environ. 2010;56(5):201-208. doi: 10.17221/231/2009-PSE.
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