Plant Soil Environ., 2013, 59(3):108-114 | DOI: 10.17221/616/2012-PSE

Impact of transgenic cottons expressing cry1Ac on soil biological attributesOriginal Paper

K. Velmourougane, A. Sahu
Central Institute for Cotton Research, Indian Council of Agricultural Research, Nagpur, Maharashtra, India

Three transgenic Bt cotton hybrids (RCH-2 Bt, Bunny Bt and NHH 44 Bt) expressing cry1Ac gene were evaluated for their effects on soil biological, microbiological and diversity attributes at 0-15 cm and 15-30 cm soil depth under field conditions. At both soil depths, soil respiration rate and fluorescein diacetate hydrolysis were the highest in the soil under Bt cotton grown followed by non-Bt soil, and by the control bulk soil, indicating no adverse effects of Bt cotton on soil microbial activity. Urease and dehydrogenase activities, reflecting potentially available N and the oxidative metabolism in soil, respectively, also increased in the sequence no-crop variant < non-Bt soil < soil under Bt cotton at both soil depths. A similar trend was found with the soil microbial biomass carbon, microbial population and microbial diversity indices. These results suggest that cultivation of Bt cotton expressing cry1Ac gene may not pose ecological or environmental risk.

Keywords: Bt cotton; dehydrogenase; microbial biomass; urease; microbial diversity indices

Published: March 31, 2013  Show citation

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Velmourougane K, Sahu A. Impact of transgenic cottons expressing cry1Ac on soil biological attributes. Plant Soil Environ. 2013;59(3):108-114. doi: 10.17221/616/2012-PSE.
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    References

    1. Anderson J.P.E. (1982): Soil respiration. In: Page A.L., Miller R.H., Keeney D.R. (eds.): Methods of Soil Analysis. Part 2 - Chemical and Microbiological Properties. 2nd Edition. ASA and SSSA, Madison, 837-871. Go to original source...
    2. Bruinsma M., Kowalchuk G.A., van Veen J.A. (2003): Effects of genetically modified plants on microbial communities and processes in soil. Biology and Fertility of Soils, 37: 329-337. Go to original source...
    3. Brusetti L., Francia P., Bertolini C., Pagliuca A., Borin S., Sorlini C., Abruzzese A., Sacchi G., Viti C., Giovannetti L., Giuntini E., Bazzicalupo M., Daffonchio D. (2005): Bacterial communities associated with the rhizosphere of transgenic Bt 176 maize (Zea mays) and its non transgenic counterpart. Plant and Soil, 266: 11-21. Go to original source...
    4. Casida L.E., Klein D.A., Santoro T. (1964): Soil dehydrogenase activity. Soil Science, 98: 371-376. Go to original source...
    5. Donegan K.K., Seidler R.J. (1999): Effects of transgenic plants on soil and plant microorganisms. In: Pandalai S.G. (ed.): Recent Research Development in Microbiology. Vol. 3, Part II, Trivandrum, India, Research Signpost, 415-424.
    6. Donegan K.K., Palm C.J., Fieland V.J., Porteous L.A., Ganio L.M., Schaller D.L., Bucao L.Q., Seidler R.J. (1995): Changes in levels, species and DNA fingerprints of soil microorganisms associated with cotton expressing the Bacillus thuringiensis var. kurstaki endotoxin. Applied Soil Ecology, 2: 111-124. Go to original source...
    7. Furczak J., Joniec J. (2007): Changes in biochemical activity of podzolic soil under willow culture in the second year of treatment with municipal-industrial sewage sludge. International Agrophysics, 21: 145-152.
    8. Garcia C., Hernandez T., Costa F. (1997): Potential use of dehydrogenase activity as an index of microbial activity in degraded soils. Communications in Soil Science and Plant Analysis, 28: 123-134. Go to original source...
    9. Griffiths B.S., Caul S., Thompson J., Birch A.N.E., Scrimgeour C., Cortet J., Foggo A., Hackett C.A., Krogh P.H. (2006): Soil microbial and faunal community responses to Bt maize and insecticide in two soils. Journal of Environmental Quality, 35: 734-741. Go to original source... Go to PubMed...
    10. Hill T.C.J., Walsh K.A., Harris J.A., Moffett B.F. (2003): Using ecological diversity measures with bacterial communities. FEMS Microbial Ecology, 43: 1-11. Go to original source... Go to PubMed...
    11. Hu H.Y., Liu X.X., Zhao Z.W., Sun J.G., Zhang Q.W., Liu X.Z., Yu Y. (2009): Effects of repeated cultivation of transgenic Bt cotton on functional bacterial populations in rhizosphere soil. World Journal of Microbiology and Biotechnology, 25: 357-366. Go to original source...
    12. Jenkinson D.S., Ladd J.N. (1985): Microbial biomass in soil, measurement and turn over. In: Paul E.A., Ladd J.N. (eds.): Soil Biochemistry. Vol. 5, Marcel Dekker, New York.
    13. Kapur M., Bhatia R., Pandey G., Pandey J., Paul D., Jain R.K. (2010): A case study for assessment of microbial community dynamics in genetically modified Bt cotton crop fields. Current Microbiology, 61: 118-124. Go to original source... Go to PubMed...
    14. Liu B., Zeng Q., Yan F., Xu H., Xu C. (2005): Effects of transgenic plants on soil microorganisms. Plant and Soil, 271: 1-13. Go to original source...
    15. Lynch J.M., Panting L.M. (1980): Cultivation and the soil biomass. Soil Biology and Biochemistry, 12: 29-33. Go to original source...
    16. Morse S., Bennett R.M., Ismael Y. (2005): Genetically modified insect resistance in cotton: Some farm level economic impacts in India. Crop Protection, 24: 433-440. Go to original source...
    17. Petras S.F., Casida L.E. Jr. (1985): Survival of Bacillus thuringiensis spores in the soil. Applied and Environmental Microbiology, 50: 28-35. Go to original source... Go to PubMed...
    18. Sarkar B., Patra A.K., Purakayastha T.J., Megharaj M. (2009): Assessment of biological and biochemical indicators in soil under transgenic Bt and non-Bt cotton crop in a sub-tropical environment. Environmental Monitoring and Assessment, 156: 595-604. Go to original source... Go to PubMed...
    19. Saxena D., Stotzky G. (2001): Bacillus thuringiensis (Bt) toxin released from root exudates and biomass of Bt corn has no apparent effect on earthworms, nematodes, protozoa, bacteria, and fungi in soil. Soil Biology and Biochemistry, 33: 1225-1230. Go to original source...
    20. Schnürer J., Rosswall T. (1982): Fluorescein diacetate hydrolysis as a measure of total microbial activity in soil and litter. Applied and Environmental Microbiology, 43: 1256-1261. Go to original source... Go to PubMed...
    21. Shen R.F., Cai H., Gong W.H. (2006): Transgenic Bt cotton has no apparent effect on enzymatic activities or functional diversity of microbial communities in rhizosphere soil. Plant and Soil, 285: 149-159. Go to original source...
    22. Singh R.J., Ahlawat I.P., Singh S. (2013): Effects of transgenic Bt cotton on soil fertility and biology under field conditions in subtropical inceptisol. Environmental Monitoring and Assessment, 185: 485-495. Go to original source... Go to PubMed...
    23. Skujins J. (1976): Enzymes in soil. In: McLaren A.D., Peterson G.H. (eds.): Soil Biochemistry. Marcel Dekker, Inc., New York, 371-414.
    24. Sun C.X., Chen L.J., Wu Z.J., Zhou L.K., Shimizu H. (2007): Soil persistence of Bacillus thuringiensis (Bt) toxin from transgenic Bt cotton tissues and its effect on soil enzyme activities. Biology and Fertility of Soils, 43: 617-620. Go to original source...
    25. Tabatabai M.A., Bremner J.M. (1972): Assay of urease activity in soils. Soil Biology and Biochemistry, 4: 479-487. Go to original source...
    26. Tabatabai M.A. (1977): Effects of trace elements on urease activity in soils. Soil Biology and Biochemistry, 9: 9-13. Go to original source...
    27. Usha M., Anita C., Anju K. (2011): Effect of Bt cotton on enzymes activity and microorganisms in rhizosphere. Journal of Agricultural Science, 3: 96-104. Go to original source...
    28. Vance E.D., Brookes P.C., Jenkinson D.S. (1987): An extraction method for measuring soil microbial biomass C. Soil Biology and Biochemistry, 19: 703-707. Go to original source...
    29. Wei X.D., Zou H.L., Chu L.M., Liao B., Ye C.M., Lan C.Y. (2006): Field released transgenic papaya affects microbial communities and enzyme activities in soil. Plant and Soil, 285: 347-358. Go to original source...
    30. Wollum A.G. (1982): Cultural methods for soil microorganisms. In: Page A.L., Mille R.H., Keeney D.R. (eds.): Methods of Soil Analysis. Part 2 - Chemical and Microbiological Properties. ASA and SSSA, Madison. Go to original source...
    31. Yan W.D., Shi W.M., Li B.H., Zhang M. (2007): Overexpression of a foreign Bt gene in cotton affects the low-molecular-weight components in root exudates. Pedosphere, 17: 324-330. Go to original source...
    32. Zaman M., Cameron K.C., Di H.J., Inubushi K. (2002): Changes in mineral N, microbial biomass and enzyme activities in different soil depths after surface applications of dairy shed effluent and chemical fertilizer. Nutrient Cycling in Agroecosystems, 63: 275-290. Go to original source...

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