Plant Soil Environ., 2016, 62(7):307-313 | DOI: 10.17221/750/2015-PSE

Variation of glucosinolates concentration and root growth of horseradish as affected by nitrogen and sulphur supplyOriginal Paper

A.R. Rivelli1, F. Lelario2, R. Agneta1, C. Möllers3, S. De Maria1
1 School of Agricultural, Forest, Food and Environmental Sciences, University of Basilicata, Potenza, Italy
2 Department of Sciences, University of Basilicata, Potenza, Italy
3 Department of Crop Sciences, University of Göttingen, Göttingen, Germany

The aim of the study was to evaluate total and individual glucosinolates concentration, root weight and diameters of horseradish accessions (Cor and Mon) grown by adding nitrogen (N) and sulphur (S) and harvested at the beginning (late autumn) and towards the end (early spring) of the harvest period. The concentration of the 9 glucosinolates (GLS) quantified in roots varied greatly between accessions (6-fold higher in Cor) and with fertilization (N alone led to an increase of 64% of GLS with an additional enhancement of 65% when S was added to N). Root GLS concentration was significantly higher in early spring compared to the previous harvest in late autumn (12.5 vs 8.9 µmol/g DW (dry weight)); a drastic decline of GLS (2.7 µmol/g DW) occurred in root harvested in the following autumn from plants left ad hoc in the field. The aliphatic sinigrin was the most abundant (80% of total GLS), followed by the aromatic gluconasturtiin and the indolic glucobrassicin (5.8% and 4.7%, respectively). Root diameter and weight increased in plants treated with fertilizers (19% and 61%, respectively) and throughout the harvests with Cor showing values double with respect to Mon (925 vs 476 g/plant). Relationships between GLS concentration and root weight (R2 = 0.61) and diameter (R2 = 0.69) were improved by excluding the roots harvested from plants left ad hoc in the field that showed the worst performances.

Keywords: above and below-ground tissues; Armoracia rusticana; medicinal herb; natural preservative; secondary metabolites

Published: July 31, 2016  Show citation

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Rivelli AR, Lelario F, Agneta R, Möllers C, De Maria S. Variation of glucosinolates concentration and root growth of horseradish as affected by nitrogen and sulphur supply. Plant Soil Environ. 2016;62(7):307-313. doi: 10.17221/750/2015-PSE.
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    References

    1. Agneta R., Lelario F., De Maria S., Möllers C., Bufo S.A., Rivelli A.R. (2014a): Glucosinolate profile and distribution among plant tissues and phenological stages of field-grown horseradish. Phytochemistry, 106: 178-187. Go to original source... Go to PubMed...
    2. Agneta R., Möllers C., De Maria S., Rivelli A.R. (2014b): Evaluation of root yield traits and glucosinolate concentration of different Armoracia rusticana accessions in Basilicata region (southern Italy). Scientia Horticulturae, 170: 249-255. Go to original source...
    3. Agneta R., Möllers C., Rivelli A.R. (2013): Horseradish (Armoracia rusticana), a neglected medical and condiment species with a relevant glucosinolate profile: A review. Genetic Resource and Crop Evolution, 60: 1923-1943. Go to original source...
    4. Alnsour M., Kleinwächter M., Böhme J., Selmar D. (2012): Sulfate determines the glucosinolate concentration of horseradish in vitro plants (Armoracia rusticana Gaertn., Mey. & Scherb.). Journal of the Science of Food and Agriculture, 93: 918-923. Go to original source... Go to PubMed...
    5. Björkman M., Klingen I., Birch A.N., Bones A.M., Bruce T.J., Johansen T.J., Meadow R., Molmann J., Seljasen R., Smart L.E., Stewart D. (2011): Phytochemicals of Brassicaceae in plant protection and human health - Influences of climate, environment and agronomic practice. Phytochemistry, 72: 538-556. Go to original source... Go to PubMed...
    6. De Maria S., Agneta R., Lelario F., Möllers C., Rivelli A.R. (2016): Influence of nitrogen and sulfur fertilization on glucosinolate content and composition of horseradish plants harvested at different developmental stages. Acta Physiologiae Plantarum, 38: 1-12. Go to original source...
    7. Falk K.L., Tokuhisa J.G., Gershenzon J. (2007): The effect of sulfur nutrition on plant glucosinolate content: Physiology and molecular mechanisms. Plant Biology, 9: 573-581. Go to original source... Go to PubMed...
    8. Food and Drug Administration (2008): Everything Added to Food in the United States (EAFUS) Available at: http://www.fda.gov/Food/FoodIngredientsPackaging/ucm115326.htm
    9. Ku K.M., Jeffery E.H., Juvik J.A., Kushad M.M. (2015): Correlation of quinone reductase activity and allyl isothiocyanate formation among different genotypes and grades of horseradish roots. Journal of Agriculture Food and Chemistry, 63: 2947-2955. Go to original source... Go to PubMed...
    10. Lelario F., De Maria S., Agneta R., Möllers C., Bufo S.A., Rivelli A.R. (2015): Glucosinolates determination in tissues of horseradish plant. Bio-Protocol, 5: e1562. Go to original source...
    11. Li X., Kushad M.M. (2004): Correlation of glucosinolate content to myrosinase activity in horseradish (Armoracia rusticana). Journal of Agricultural and Food Chemistry, 52: 6950-6955. Go to original source... Go to PubMed...
    12. Nguyen N.M., Gonda S., Vasas G. (2013): A review on the phytochemical composition and potential medicinal uses of horseradish (Armoracia rusticana) root. Food Reviews International, 29: 261-275. Go to original source...
    13. Perlaki Z., Djurovka M. (2009): Horseradish root yield depending on organic and mineral fertilizers. Contemporary Agriculture, 58: 106-111.
    14. Sultana T., Savage G.P., McNeil D.L., Porter N.G., Martin R.J., Deo B. (2002): Effects of fertilisation on the allyl isothiocyanate profile of above-ground tissues of New Zealand-grown wasabi. Journal of the Science of Food and Agriculture, 82: 1477-1482. Go to original source...
    15. UPOV International Union for the Protection of New Varieties of Plants (2001): Guidelines for the Conduct of Tests for Distinctness, Uniformity and Stability. Horseradish (Armoracia rusticana Gaertn, Mey. & Scherb.). Geneva, TG/191/2.
    16. Velasco P., Cartea M.E., Gonzales C., Vilar M., Ordas A. (2007): Factors affecting the glucosinolates content of kale (Brassica oleracea acephala group). Journal of Agriculture and Food Chemistry, 55: 955-962. Go to original source... Go to PubMed...
    17. Walters S.A., Wahle E.A. (2010): Horseradish production in Illinois. HortTechnology, 20: 267-276. Go to original source...
    18. Wedelsbäck Bladh K. (2014): Biodiversity in nordic horseradish (Armoracia rusticana). [Ph.D. Thesis] Alnarp, Swedish University of Agricultural Sciences. Go to original source...
    19. Zukalová H., Va¹ák J. (2002): The role and effects of glucosinolates of Brassica species - A review. Rostlinná Výroba, 48: 175-180. Go to original source...

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