Plant Soil Environ., 2017, 63(11):519-524 | DOI: 10.17221/617/2017-PSE

The effect of inoculation with arbuscular mycorrhizal fungus Rhizophagus irregularis on cytokinin content in a highly mycotrophic Medicago lupulina line under low phosphorus level in the soilOriginal Paper

Andrey YURKOV1,2,3, Svetlana VESELOVA4, Lidia JACOBI1, Galina STEPANOVA5, Vladislav YEMELYANOV3, Guzel KUDOYAROVA6, Maria SHISHOVA*,3
1 All-Russian Research Institute for Agricultural Microbiology, Federal Agency of Scientific Organizations, St. Petersburg, Russia
2 Russian State Hydrometeorological University, Ministry of Education and Science of the Russian Federation, Saint-Petersburg, Russia
3 Saint-Petersburg State University, Saint-Petersburg, Russia
4 Institute of Biochemistry and Genetics of Ufa Scientific Center, Russian Academy of Sciences, Federal Agency of Scientific Organizations, Oktyabrya, Russia
5 All-Russian Williams Fodder Research Institute, Federal Agency of Scientific Organizations, Lobnya, Russia
6 Institute of Biology of Ufa Scientific Center, Russian Academy of Sciences, Federal Agency of Scientific Organizations, Ufa, Russia

The study is focused on the elucidation of the role of cytokinins (CKs, zeatin and zeatin riboside) in the development of effective arbuscular mycorrhiza (AM) symbiosis with Medicago lupulina. An important mechanism involved in the regulation of host plant growth is supposed to be linked to the modulation of plant hormone balance. The data obtained revealed the formation of an effective AM-symbiosis (M. lupulina + Rhizophagus irregularis) under phosphorus-deficiency. At the shooting stage (35th day after sowing), it is characterized by a decrease in the root:shoot ratio, the lowering in arbuscules and vesicle abundances, but an increase in the intensity of mycelium development. Mycorrhized plants differed from the control ones by higher CK levels in both roots and leaves. Zeatin and zeatin riboside concentration exhibited uneven alterations over time. A role of mycelium in the modulation of CK balance has been discussed.

Keywords: Fabaceae; phytohormone; rhizosphere; macronutrient; arbuscular mycorrhizae

Published: November 30, 2017  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
YURKOV A, VESELOVA S, JACOBI L, STEPANOVA G, YEMELYANOV V, KUDOYAROVA G, SHISHOVA M. The effect of inoculation with arbuscular mycorrhizal fungus Rhizophagus irregularis on cytokinin content in a highly mycotrophic Medicago lupulina line under low phosphorus level in the soil. Plant Soil Environ. 2017;63(11):519-524. doi: 10.17221/617/2017-PSE.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Adalsteinsson S., Jensén P. (1990): Modifications of root geometry in winter wheat by phosphorus deprivation. Journal of Plant Physiology, 135: 513-517. Go to original source...
    2. Alazem M., Lin N.S. (2015): Roles of plant hormones in the regulation of host-virus interactions. Molecular Plant Pathology, 16: 529-540. Go to original source... Go to PubMed...
    3. Arata Y., Nagasawa-Iida A., Uneme H., Nakajima H., Kakimoto T., Sato R. (2010): The phenylquinazoline compound S-4893 a non-competitive cytokinin antagonist that targets Arabidopsis cytokinin receptor CRE1 and promotes root growth in Arabidopsis and rice. Plant and Cell Physiology, 51: 2047-2059. Go to original source... Go to PubMed...
    4. Barea J.M., Azćon-Aguilar C. (1982): Production of plant growth regulating substances by the vesicular-arbuscular mycorrhizal fungus Glomus mosseae. Applied and Environmental Microbiology, 43: 810-813. Go to original source... Go to PubMed...
    5. Bielach A., Podlešáková K., Marhavý P., Duclercq J., Cuesta C., Müller B., Grunewald W., Tarkowski P., Benková E. (2012): Spatiotemporal regulation of lateral root organogenesis in Arabidopsis by cytokinin. The Plant Cell, 24: 3967-3981. Go to original source... Go to PubMed...
    6. Boivin S., Fonouni-Farde C., Frugier F. (2016): How auxin and cytokinin phytohormones modulate root microbe interactions. Frontiers in Plant Science, 7: 1240. Go to original source... Go to PubMed...
    7. Cosme M., Ramireddy E., Franken P., Schmülling T., Wurst S. (2016): Shoot- and root-borne cytokinin influences arbuscular mycorrhizal symbiosis. Mycorrhiza, 26: 709-720. Go to original source... Go to PubMed...
    8. Danneberg G., Latus C., Zimmer W., Hundeshagen B., SchneiderPoetsch Hj., Bothe H. (1993): Influence of vesicular-arbuscular mycorrhiza on phytohormone balances in maize (Zea mays L.). Journal of Plant Physiology, 141: 33-39. Go to original source...
    9. Denison R.F., Kiers E.T. (2011): Life histories of symbiotic rhizobia and mycorrhizal fungi. Current Biology, 21: R775-785. Go to original source... Go to PubMed...
    10. Dickson S. (2004): The Arum-Paris continuum of mycorrhizal symbioses. New Phytologist, 163: 187-200. Go to original source... Go to PubMed...
    11. Drew M.C. (1975): Comparison of the effects of a localized supply of phosphate, nitrate, ammonium and potassium on the growth of the seminal root system, and the shoot, in barley. New Phytologist, 75: 479-490. Go to original source...
    12. Drüge U., Schonbeck F. (1992): Effect of vesicular-arbuscular mycorrhizal infection on transpiration, photosynthesis and growth of flax (Linum usitatissimum L.) in relation to cytokinin levels. Journal of Plant Physiology, 141: 40-48. Go to original source...
    13. Fitter A.H. (2006): What is the link between carbon and phosphorus fluxes in arbuscular mycorrhizas? A null hypothesis for symbiotic function. New Phytologist, 172: 3-6. Go to original source... Go to PubMed...
    14. Fukaki H., Tasaka M. (2009): Hormone interactions during lateral root formation. Plant Molecular Biology, 69: 437-449. Go to original source... Go to PubMed...
    15. Fusconi A. (2014): Regulation of root morphogenesis in arbuscular mycorrhizae: What role do fungal exudates, phosphate, sugars and hormones play in lateral root formation? Annals of Botany, 113: 19-33. Go to original source... Go to PubMed...
    16. Gai J.P., Feng G., Christie P., Li X.L. (2006): Screening of arbuscular mycorrhizal fungi for symbiotic efficiency with sweet potato. Journal of Plant Nutrition, 29: 1085-1094. Go to original source...
    17. Gajdošová S., Spíchal L., Kamínek M., Hoyerová K., Novák O., Dobrev P.I., Galuszka P., Klíma P., Gaudinová A., Žižková E., Hanuš J., Dančák M., Trávníček B., Pešek B., Krupička M., Vaňková R., Strnad M., Motyka V. (2011): Distribution, biological activities, metabolism, and the conceivable function of cis-zeatin-type cytokinins in plants. Journal of Experimental Botany, 62: 2827-2840. Go to original source... Go to PubMed...
    18. Hodge A., Berta G., Doussan C., Merchan F., Crespi M. (2009): Plant root growth, architecture and function. Plant and Soil, 321: 153-187. Go to original source...
    19. Kudoyarova G.R., Korobova A.V., Akhiyarova G.R., Arkhipova T.N., Zaytsev D.Y., Prinsen E., Egutkin N.L., Medvedev S.S., Veselov S.Y. (2014): Accumulation of cytokinins in roots and their export to the shoots of durum wheat plants treated with the protonophore carbonyl cyanide m-chlorophenylhydrazone (CCCP). Journal of Experimental Botany, 65: 2287-2294. Go to original source... Go to PubMed...
    20. Liu J., Guo C., Chen Z.-L., He J.-D., Zou Y.-N. (2016): Mycorrhizal inoculation modulates root morphology and root phytohormone responses in trifoliate orange under drought stress. Emirates Journal of Food and Agriculture, 28: 251-256. Go to original source...
    21. López-Bucio J., Hernández-Abreu E., Sánchez-Calderón L., NietoJacobo M.F., Simpson J., Herrera-Estrella L. (2002): Phosphate availability alters architecture and causes changes in hormone sensitivity in the Arabidopsis root system. Plant Physiology, 129: 244-256. Go to original source... Go to PubMed...
    22. Mollier A., Pellerin S. (1999): Maize root system growth and development as influenced by phosphorus deficiency. Journal of Experimental Botany, 50: 487-497. Go to original source...
    23. Phillips J.M., Hayman D.S. (1970): Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection. Transactions of the British Mycological Society, 55: 158-161. Go to original source...
    24. Price N.S., Roncadori R.W., Hussey R.S. (1989): Cotton root growth as influenced by phosphorus nutrition and vesiculararbuscular mycorrhizas. New Phytologist, 111: 61-66. Go to original source...
    25. Ryan M.H., McCully M.E., Huang C.X. (2003): Location and quantification of phosphorus and other elements in fully hydrated, soil-grown arbuscular mycorrhizas: A cryo-analytical scanning electron microscopy study. New Phytologist, 160: 429-441. Go to original source... Go to PubMed...
    26. Schaarschmidt S., González M.-C., Roitsch T., Strack D., Sonnewald U., Hause B. (2007): Regulation of arbuscular mycorrhization by carbon. The symbiotic interaction cannot be improved by increased carbon availability accomplished by root-specifically enhanced invertase activity. Plant Physiology, 143: 1827-1840. Go to original source...
    27. Schmidt C.S., Mrnka L., Frantík T., Motyka V., Dobrev P.I., Vosátka M. (2017): Combined effects of fungal inoculants and the cytokinin-like growth regulator thidiazuron on growth, phytohormone contents and endophytic root fungi in Miscanthus × giganteus. Plant Physiology and Biochemistry, 120: 120-131. Go to original source... Go to PubMed...
    28. Shaul-Keinan O., Gadkar V., Ginzberg I., Grünzweig J.M., Chet I., Elad Y., Wininger S., Belausov E., Eshed Y., Atzmon N., BenTal Y., Kapulnik Y. (2002): Hormone concentrations in tobacco roots change during arbuscular mycorrhizal colonization with Glomus intraradices. New Phytologist, 154: 501-507. Go to original source... Go to PubMed...
    29. Smith S.E., Read D.J. (2008): Mycorrhizal Symbiosis. 3 rd Edition. New York, Academic Press, 800.
    30. Torelli A., Trotta A., Acerbi L., Arcidiacono G., Berta G., Branca C. (2000): IAA and ZR content in leek (Allium porrum L.) as influenced by P nutrition and arbuscular mycorrhizae, in relation to plant development. Plant and Soil, 226: 29-35. Go to original source...
    31. Trouvelot A., Kough J.L., Gianinazzi-Pearson V. (1986): Mesure du taux de mycorhization VA d'un système radiculaire. Recherche de méthodes ayant une signification fonctionnelle. In: Gianinazzi-Pearson V., Gianinazzi S. (eds.): Physiological and Genetical Aspects of Mycorrhizae. Paris, INRA-Press, 217-221.
    32. Vysotskaya L.B., Korobova A.V., Veselov S.Y., Dodd I.C., Kudoyarova G.R. (2009): ABA mediation of shoot cytokinin oxidase activity: Assessing its impacts on cytokinin status and biomass allocation of nutrient deprived durum wheat. Functional Plant Biology, 36: 66-72. Go to original source... Go to PubMed...
    33. Yurkov A., Shishova M., Semenov D. (2010): Features of Development of Black Medick with Endomycorrhizal Fungus. Saarbrucken, Lambert Academic Publishing GmBH, 215.
    34. Yurkov A.P., Jacobi L.M., Gapeeva N.E., Stepanova G.V., Shishova M.F. (2015): Development of arbuscular mycorrhiza in highly responsive and mycotrophic host plant-black medick (Medicago lupulina L.). Ontogenez, 46: 313-326. (In Russian) Go to original source... Go to PubMed...
    35. Yurkov A.P., Veselova S.V., Jacobi L.M., Stepanova G.V., Kudoyarova G.R., Shishova M.F. (2017): The effect of inoculation with arbuscular mycorrhizal fungus Rhizophagus irregularis on auxin content in highly mycotrophic black medick under low phosphorus level in soil. Sel'skokhozyaistvennaya biologiya [Agricultural Biology], 52: 830-838. Go to original source...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content