Plant Soil Environ., 2018, 64(8):386-392 | DOI: 10.17221/110/2018-PSE

Detecting herbicide-resistant Apera spica-venti with a chlorophyll fluorescence agar testOriginal Paper

Alexander Ingo LINN*,1, Pavlína KO©NAROVÁ2, Josef SOUKUP2, Roland GERHARDS1
1 Department of Weed Science, Institute of Phytomedicine, University of Hohenheim, Stuttgart, Germany
2 Department of Agroecology and Biometeorology, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic

Reliable tests on herbicide resistance are important for resistance management. Despite well-established greenhouse bioassays, faster and in-season screening methods would aid in more efficient resistance detection. The feasibility of a chlorophyll fluorescence agar-based test on herbicide resistance in Apera spica-venti L. was investigated. Herbicide resistant and sensitive A. spica-venti seedlings were transplanted into agar containing pinoxaden and pyroxsulam herbicides. Chlorophyll fluorescence was measured and the maximum quantum efficiency of photosystem II (Fv/Fm) was determined 48 h and 72 h after the transplantation to agar, respectively. The Fv/Fm values decreased with increasing herbicide concentration. Dose-response curves and respective ED50 values (herbicide concentration leading to 50% decrease of the Fv/Fm value) were calculated. However, each experiment repetition exhibited different sensitivities of the populations for both herbicides. In certain cases, resistant populations demonstrated similar Fv/Fm values as sensitive populations. Contrary to the findings in Alopecurus myosuroides Huds., discrimination of sensitive and resistant A. spica-venti populations was not feasible. An increased importance of the assessment time due to the herbicide concentrations calibrated for fast responses was assumed in this study.

Keywords: ALS inhibitor; ACCase inhibitor; herbicide-resistant weed; rapid detection; laboratory study; PAM fluorimeter

Published: August 31, 2018  Show citation

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LINN AI, KO©NAROVÁ P, SOUKUP J, GERHARDS R. Detecting herbicide-resistant Apera spica-venti with a chlorophyll fluorescence agar test. Plant Soil Environ. 2018;64(8):386-392. doi: 10.17221/110/2018-PSE.
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