Plant Soil Environ., 2020, 66(3):113-118 | DOI: 10.17221/14/2020-PSE

Soil and foliar zinc application to biofortify broccoli (Brassica oleracea var. italica L.): effects on the zinc concentration and bioavailabilityOriginal Paper

Angelica Rivera-Martin1, Martin R. Broadley2, Maria J. Poblaciones ORCID...*,1,2
1 Department of Agronomy and Forest Environment Engineering, University of Extremadura, Badajoz, Spain
2 School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, UK

Agronomic zinc (Zn) biofortification of crops could help to alleviate dietary Zn deficiency, which is likely to affect more than one billion people worldwide. To evaluate the efficiency of agronomic Zn biofortification of broccoli, four application treatments were tested: no Zn application (control); soil application of 5 mg/kg ZnSO4.7 H2O (soil); two sprays (15 mL/pot each) of 0.25% (w/v) ZnSO4.7 H2O (foliar); and soil + foliar combination. Soil Zn application increased Zn-DTPA (diethylenetriamine pentaacetic acid) concentration by 3.7-times but did not affect plant growth or plant Zn concentration. Foliar Zn application increased stem + leaves and floret Zn concentration by 78 and 23 mg Zn/kg, respectively, with good bioavailability based on phytic acid concentration. Boiling decreased mineral concentration by 19%, but increased bioavailability by decreasing the phytic acid concentration. The entire broccoli could constitute a good nutritional source for animals and humans. An intake of 100 g boiled florets treated with the foliar treatment will cover about 36% of recommended dietary intake (RDI) of Zn, together with 30% of Ca, 94% of K, 32% of Mg, 6% of Na, 55% of P, 60% of S, 10% of Cu, 22% of Fe, 43% of Mn, and 35% of Se RDIs.

Keywords: bioavailability; nutrient uptake; zinc fertiliser; Brassica; phytate

Published: March 31, 2020  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Rivera-Martin A, Broadley MR, Poblaciones MJ. Soil and foliar zinc application to biofortify broccoli (Brassica oleracea var. italica L.): effects on the zinc concentration and bioavailability. Plant Soil Environ. 2020;66(3):113-118. doi: 10.17221/14/2020-PSE.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Abd El-All H.M. (2014): Improving growth, yield, quality, and sulphoraphane content as anticancer of broccoli (Brassica oleracea L. var. italica) plants by some fertilization treatments. Middle East Journal of Agriculture Research, 3: 13-19.
    2. Cakmak I., Kalayci M., Kaya Y., Torun A.A., Aydin N., Wang Y., Arisov Z., Erdem H., Yazici A., Gokmen O., Ozturk L., Horst W.J. (2010): Biofortification and localization of zinc in wheat grain. Journal of Agriculture and Food Chemistry, 58: 9092-9102. Go to original source... Go to PubMed...
    3. Evans W.J., Martin C.J. (1988): Interactions of Mg(II), Co(II), Ni(II), and Zn(II) with phytic acid. VIII. A calorimetric study. Journal of Inorganic Biochemistry, 32: 259-268. Go to original source...
    4. FAO/WHO (2000): Vitamin and mineral requirements in human nutrition. Available at: https://apps.who.int/iris/bitstream/handle/10665/42716/9241546123.pdf;jsessionid=F3154A4C1EE7BE67604532409AB9199E?sequence=1 (accessed 17. 12. 2019)
    5. Francisco M., Tortosa M., Martínez-Ballesta M.del C., Velasco P., García-Viguera C., Moreno D.A. (2017): Nutritional and phytochemical value of Brassica crops from the agri-food perspective. Annals of Applied Biology, 170: 273-285. Go to original source...
    6. Gibson R.S. (2007): The role of diet- and host-related factors in nutrient bioavailability and thus in nutrient-based dietary requirement estimates. Food and Nutrition Bulletin, 28: S77-100. Go to original source... Go to PubMed...
    7. Gomez-Coronado F., Poblaciones M.J., Almeida A.S., Cakmak I. (2016): Zinc (Zn) concentration of bread wheat grown under Mediterranean conditions as affected by genotype and soil/foliar Zn application. Plant and Soil, 401: 331-346. Go to original source...
    8. Hallberg L., Brune M., Rossander L. (1989): Iron absorption in man: ascorbic acid and dose-dependent inhibition by phytate. American Journal of Clinical Nutrition, 49: 140-144. Go to original source... Go to PubMed...
    9. Huett D.O., Maier N.A., Sparrow L.A., Piggot T.J. (1997): Vegetable crops. In: Reuter D.J., Robinson J.B. (eds.): Plant Analysis: An Interpretation Manual. 2nd Edition. Collingwood, Commonwealth Scientific and Industrial Research Organisation, 383-464.
    10. Ka³u¿ewicz A., Bosiacki M., Fr±szczak B. (2016): Mineral composition and the content of phenolic compounds of ten broccoli cultivars. Journal of Elementology, 21: 53-65.
    11. Lindsay W.L., Norvell W.A. (1978): Development of a DTPA soil test for zinc, iron, manganese and copper. Soil Science Society of America Journal, 42: 421-428. Go to original source...
    12. Liu M., Zhang L., Ser S.L., Cumminq J.R., Ku K.M. (2018): Comparative phytonutrient analysis of broccoli by-products: the potentials for broccoli by-product utilization. Molecules, 23: 9-18. compounds of broccoli. Journal of Pharmaceutical and Biomedical Analysis, 41: 1508-1522. Go to original source... Go to PubMed...
    13. Morris E.R., Ellis R. (1989): Usefulness of the dietary phytic acid/ zinc molar ratio as an index of zinc bioavailability to rats and humans. Biological Trace Element Research, 19: 107-117. Go to original source... Go to PubMed...
    14. Ogbede S.C., Saidu A.N., Kabiru A.Y., Busari M.B. (2015): Nutrient and anti-nutrient compositions of Brassica oleraceae var. capitata L. IOSR Journal of Pharmacy, 5: 19-25.
    15. Poblaciones M.J., Rengel Z. (2017a): Soil and foliar zinc biofortification in field pea (Pisum sativum L.): Grain accumulation and bioavailability in raw and cooked grains. Food Chemistry, 212: 427-433. Go to original source... Go to PubMed...
    16. Poblaciones M.J., Rengel Z. (2017b): Combined foliar selenium and zinc biofortification in field pea (Pisum sativum): accumulation and bioavailability in raw and cooked grains. Crop and Pasture Science, 68: 265-271. Go to original source...
    17. Reason D.A., Watts M.J., Devez A., Broadley M.R. (2015): Quantification of phytic acid in grains. British Geological Survey Open Report, OR/15/070, 18.
    18. Sánchez C., López-Jurado M., Planells E., Llopis J., Aranda P. (2009): Assessment of iron and zinc intake and related biochemical parameters in an adult Mediterranean population from southern Spain: influence of lifestyle factors. Journal of Nutrition Biochemistry, 20: 125-131. Go to original source... Go to PubMed...
    19. Sandström B. (1989): Dietary pattern and zinc supply. In: Mills C.F. (ed.): Zinc in Human Biology. Devon, Springer-Verlag, 350-363. Go to original source...
    20. Sims J.T., Johnson G.V. (1991): Micronutrient soil test in micronutrients in agriculture. In: Mordvedt J.J. (ed.): Micronutrients in Agriculture. 2nd Edition, Madison, Soil Science Society of America Book Series No. 4, 427-476. Go to original source...
    21. ©losár M., Mezeyová I., Hegedüsová A., Andrejiová A., Kováèik P., Lo¹ák T., Kopta T., Keutgen A. (2017): Effect of zinc fertilisation on yield and selected qualitative parameters of broccoli. Plant, Soil and Environment, 63: 282-287. Go to original source...
    22. Thavarajah D., Ruszkowski J., Vandenberg A. (2008): High potential for selenium biofortification of lentils (Lens culinaris L.). Journal of Agriculture and Food Chemistry, 56: 10747-10753. Go to original source... Go to PubMed...
    23. White P.J., Pongrac P., Sneddon C.C., Thompson J.A., Wright G. (2018): Limits to the biofortification of leafy Brassicas with zinc. Agriculture, 8: 32. Go to original source...
    24. WHO (2016): Vitamin and Mineral Nutrition Information System. Geneva, World Health Organization. Available at: www.who.int
    25. Zhao A.Q., Yang S., Wang B., Tian X., Zhang Y. (2018): Effects of ZnSO4 and Zn-EDTA broadcast or banded to soil on Zn bioavailability in wheat (Triticum aestivum L.) and Zn fractions in soil. Chemosphere, 205: 350-360. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content