Plant Soil Environ., 2020, 66(7):309-316 | DOI: 10.17221/137/2020-PSE

Bacterial endophytes from horseradish (Armoracia rusticana G. Gaertn.,B.Mey.&Scherb.) with antimicrobial efficacy against pathogensOriginal Paper

Dilfuza Egamberdieva ORCID...*,1,2, Vyacheslav Shurigin2, Burak Alaylar3, Stephan Wirth1, Sonoko Dorothea Bellingrath-Kimura1,4
1 Leibniz Centre for Agricultural Landscape Research (ZALF), Müncheberg, Germany
2 Faculty of Biology, National University of Uzbekistan, Tashkent, Uzbekistan
3 Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Agri Ibrahim Cecen University, Agri, Turkey
4 Faculty of Life Science, Humboldt University of Berlin, Berlin, Germany

The current study aimed to determine the diversity of culturable endophytic bacteria associated with horseradish (Armoracia rusticana G.Gaertn., B.Mey.&Scherb.) grown in Chatkal Biosphere Reserve of Uzbekistan and their antimicrobial potentials. The bacteria were isolated from plant leaves and root tissues using culture-dependent techniques. The 16S rRNA sequences similarities of endophytic bacteria isolated from A. rusticana showed that isolates belong to species Paenibacillus, Raoultella, Stenotrophomonas, Pseudomonas, Serratia, Microbacterium, Enterobacter, Achromobacter, Brevibacterium, Pantoea, and Erwinia. The isolated endophytic bacteria Stenotrophomonas maltophilia KRT1, Serratia ficaria KRT5, and Pantoea agglomerans KLT4 possess antimicrobial activities against human pathogenic bacteria Staphylococcus aureus, Escherichia coli, and Candida albicans. The endophytic bacteria Paenibacillus typhae KRN1, Stenotrophomonas maltophilia KRT1, Pseudomonas kilonensis KRT11, Pseudomonas umsongensis KRT21, Brevibacterium frigoritolerans KLT2 and Pantoea agglomerans KLT4 inhibited phytopathogenic fungi Rhizoctonia solani, Fusarium culmorum, and F. solani. These findings indicate that plant endophytic bacteria with antimicrobial activity could be a source for producing agriculturally and pharmaceutically important antimicrobial compounds.

Keywords: medicinal plant; Brassicaceae; plant microbiome; bacterial diversity

Published: July 31, 2020  Show citation

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Egamberdieva D, Shurigin V, Alaylar B, Wirth S, Bellingrath-Kimura SD. Bacterial endophytes from horseradish (Armoracia rusticana G. Gaertn.,B.Mey.&Scherb.) with antimicrobial efficacy against pathogens. Plant Soil Environ. 2020;66(7):309-316. doi: 10.17221/137/2020-PSE.
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