Plant Soil Environ., 2021, 67(4):208-220 | DOI: 10.17221/659/2020-PSE

Exogenous application of melatonin alleviates salt stress-induced decline in growth and photosynthesis in Glycine max (L.) seedlings by improving mineral uptake, antioxidant and glyoxalase systemOriginal Paper

Basmah M. Alharbi1, Abeer Hamdy Elhakem2, Ghalia S.H. Alnusairi3,4, Mona H. Soliman5,6, Khalid Rehman Hakeem7, Md. Mahadi Hasan ORCID...8, *, Magdi T. Abdelhamid9
1 Department of Biology, Faculty of Science, University of Tabuk, Tabuk, Saudi Arabia
2 Department of Biology, College of Sciences and Humanities in AlKharj, Prince Sattam Bin Abdulaziz University, AlKharj, Saudi Arabia
3 Department of Biology, College of Science, Jouf University, Sakaka, Saudi Arabia
4 Department of Biology, College of Science, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia
5 Botany and Microbiology Department, Faculty of Science, Cairo University, Giza, Egypt
6 Biology Department, Faculty of Science, Taibah University, Al-Sharm, Yanbu El-Bahr, Yanbu, Saudi Arabia
7 Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia
8 State Key Laboratory of Grassland Agro-Ecosystems, School of Life Sciences, Lanzhou University, Lanzhou, P.R. China
9 Botany Department, National Research Centre, Cairo, Egypt

Soybean plants grown under NaCl were foliar sprayed twice with melatonin (MLT). Results revealed that salt stress reduced growth, biomass accumulation, photosynthesis, mineral uptake, the content of δ-aminolevulinic acid, chlorophylls, carotenoids and PSII efficiency. However, MLT application enhanced pigment synthesis and PSII activity. MLT up-regulated the antioxidant system and glyoxalase function resulting in reduced accumulation of reactive oxygen species (ROS). Reduced ROS in MLT-treated plants protected membrane functioning by reducing lipid peroxidation, electrolyte leakage and lipoxygenase activity. Nevertheless, MLT application reduced methylglyoxal accumulation while increased the content of reduced glutathione and ascorbic acid. It could be concluded that exogenous MLT mitigated the salt stress damage in soybean plants by improving photosynthesis, antioxidant systems, controlling ion homeostasis and minimising excessive ROS accumulation.

Keywords: salinity tolerance mechanisms; osmolyte; malondialdehyde; phytohormone; secondary metabolites; ascorbate

Published: April 30, 2021  Show citation

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Alharbi BM, Elhakem AH, Alnusairi GSH, Soliman MH, Hakeem KR, Hasan MM, Abdelhamid MT. Exogenous application of melatonin alleviates salt stress-induced decline in growth and photosynthesis in Glycine max (L.) seedlings by improving mineral uptake, antioxidant and glyoxalase system. Plant Soil Environ. 2021;67(4):208-220. doi: 10.17221/659/2020-PSE.
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