Plant Soil Environ., 2022, 68(7):309-316 | DOI: 10.17221/87/2022-PSE

Sensitivity of fast chlorophyll fluorescence parameters to combined heat and drought stress in wheat genotypesOriginal Paper

Mária Barboričová ORCID..., Andrej Filaček ORCID..., Dominika Mlynáriková Vysoká, Kristína Gašparovič ORCID..., Marek Živčák*, Marián Brestič ORCID...
Institute of Plant and Environmental Sciences, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic

This study aimed to characterise the specific phenotypic responses and the sensitivity of photosynthetic parameters to progressive drought in modern wheat genotypes. In pot experiments, we tested eight wheat genotypes (Triticum sp.) that differed in ploidy level and country of origin. Water stress was simulated by the restriction of irrigation, which led to a decreased leaf relative water content of up to 70%. During gradual dehydration, changes in the structure and function of photosystem II (PSII) were analysed using the fluorescence parameters derived from fast fluorescence kinetics (OJIP transient). The results indicated that a group of JIP test-based parameters demonstrated sensitivity to drought, including genotype-specific responses. Severe drought stress led to a decrease in the photochemical efficiency of PSII (Fv/Fm), a reduction in the number of active PSII reaction centers (RC/ABS) and a decrease in parameters, indicating overall photochemical performance at the PSII level (performance indices PIabs and PItot). These findings demonstrate that the approaches used in our experiments were useful and reliable in monitoring the physiological responses of individual varieties of wheat exposed to stress conditions, and they have application potential as selection criteria in crop breeding. The contribution of the high-temperature effects on the photochemical responses under water deficit conditions is also discussed.

Keywords: photosynthesis; non-invasive methods; stress tolerance; environmental constraints; crop physiology

Published: July 15, 2022  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Barboričová M, Filaček A, Mlynáriková Vysoká D, Gašparovič K, Živčák M, Brestič M. Sensitivity of fast chlorophyll fluorescence parameters to combined heat and drought stress in wheat genotypes. Plant Soil Environ. 2022;68(7):309-316. doi: 10.17221/87/2022-PSE.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Araus J.L., Caims J.E. (2014): Field high-throughput phenotyping: the new crop breeding frontier. Journal of Trends in Plant Science, 19: 52-61. Go to original source... Go to PubMed...
    2. Ayeneh A., van Ginkel M., Reynolds M.P., Ammar K. (2002): Comparison of leaf, spike, peduncle and canopy temperature depression in wheat under heat stress. Field Crops Research, 79: 173-184. Go to original source...
    3. Botyanszka L. (2019): Development of phenotyping methods and diagnostic approaches useful in identification of the tolerant crop genetic resources. [Dissertation Thesis] Nitra, Slovak University of Agriculture.
    4. Brestic M., Zivcak M. (2013): PSII fluorescence techniques for measurement of drought and high temperature stress signal in crop plants: protocols and applications. In: Rout G.R., Das A.B. (eds.): Molecular Stress Physiology of Plants. Dordrecht, Springer, 87-131. ISBN: 9788132208075 Go to original source...
    5. Brestic M., Živcak M., Hauptvogel P., Misheva S., Kocheva K., Yang X., Li X., Allakhverdiev S.I. (2018): Wheat plant selection for high yields entailed improvement of leaf anatomical and biochemical traits including tolerance to non-optimal temperature
    6. Chaves M.M., Flexas J., Pinheiro C. (2008): Photosynthesis under drought and salt stress: regulation mechanisms from whole plant to cell. Annals of Botany, 103: 551-560. Go to original source... Go to PubMed...
    7. Chen Y.E., Su Y.Q., Zhang C.M., Ma J., Mao H.T., Yang Z.H., Yuan M., Zhang Z.W., Yuan S., Zhang H.Y. (2017): Comparison of photosynthetic characteristics and antioxidant systems in different wheat strains. Journal of Plant Growth Regulation, 37: 347-359. Go to original source...
    8. Chovancek E., Zivcak M., Botyanszka L., Hauptvogel P., Yang X., Misheva S., Hussain S., Brestic M. (2019): Transient heat waves may affect the photosynthetic capacity of susceptible wheat genotypes due to insufficient photosystem I photoprotection. Plants, 8: 282. Go to original source... Go to PubMed...
    9. Farooq M., Hussain M., Siddique K.H. (2014): Drought stress in wheat during flowering and grain-filling periods. Critical Re
    10. Ferroni L., Živčak M., Sytar O., Kovár M., Watanabe N., Pancaldi S., Brestič M. (2020): Chlorophyll-depleted wheat mutants are disturbed in photosynthetic electron flow regulation but can retain an acclimation ability to a fluctuating light regime. Environmental and Experimental Botany, 178: 104156. Go to original source...
    11. Goltsev V., Zaharieva I., Chernev P., Kouzmanova M., Kalaji H.M., Yordanov I., Krasteva V., Alexandrov V., Stefanov D., Allakhverdiev S.I., Strasser R.J. (2012): Drought-induced modifications of photosynthetic electron transport in intact leaves: analysis and use of neural networks as a tool for a rapid non-invasive estimation. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1817: 1490-1498. Go to original source... Go to PubMed...
    12. Hussain S., Ulhassan Z., Brestic M., Zivcak M., Zhou W., Allakhverdiev S.I., Yang X., Safdar M.E., Yang W., Liu W. (2021): Photosynthesis research under climate change. Photosynth Research, 150: 5-19. Go to original source... Go to PubMed...
    13. Jedmowski C., Bayramov S., Brügemann W. (2014): Comparative analysis of drought stress effects on photosynthesis of Eurasian and North African genotypes of wild barley. Photosynthetica, 52: 564-573. Go to original source...
    14. Kaiser W.M. (1987): Effect of water deficit on photosynthetic capacity. Physiologia Plantarum, 71: 142-149. Go to original source...
    15. Kalaji H.M., Oukarroum A., Alexandrov V., Kouzmanova M., Brestic M., Zivcak M., Samborska I.A., Cetner M.D., Allakhverdiev S.I., Goltsev V. (2014): Identification of nutrient deficiency in maize and tomato plants by in vivo chlorophyll a fluorescence measurements. Journal of Plant Physiology and Biochemistry, 81: 16-25. Go to original source... Go to PubMed...
    16. Kalaji H.M., Schansker G., Brestic M., Bussotti F., Calatayud A., Ferroni L., Goltsev V., Guidi L., Jajoo A., Li P., Losciale P., Mishra V.K., Misra A.N., Nebauer S.G., Pancaldi S., Penella C., Pollastrini M., Suresh K., Tambussi E., Yanniccari M., Zivcak M., Cetner M.D., Rusinowski I.A., Baba W. (2017): Frequently asked questions about chlorophyll fluorescence, the sequel. Photosynthesis Research, 132: 13-66. Go to original source... Go to PubMed...
    17. Long S.P., Colon A.M., Zhu X.G. (2015): Meeting the global food demand of the future by engineering crop photosynthesis and yield potential. Journal of Cell, 161: 56-66. Go to original source... Go to PubMed...
    18. Lu C., Zhang J. (1999): Effect of water stress on photosystem II photochemistry and its thermostability in wheat plants. Journal of Experimental Botany, 336: 1199-1206. Go to original source...
    19. Massaci A., Battistelli A., Loreto F. (1996): Effect of drought stress on photosynthetic characteristics, growth and sugar accumulation of field-grown sweet sorghum. Journal of Functional Plant Biology, 23: 331-340. Go to original source...
    20. Monneveux P., Jing R., Misra S.C. (2012): Phenotyping for drought adaptation in wheat using physiological traits. Frontiers in Physiology, 3: 429. Go to original source... Go to PubMed...
    21. Psidova E., Zivcak M., Stojnić S., Orlović S., Gőmőry D., Kucerova J., Ditmarova L., Střelcova K., Brestic M., Kalaji H.M. (2018): Altitude of origin influences the responses of PSII photochemistry to heat waves in European beech (Fagus sylvatica L.). Journal of Environmental and Experimental Botany, 152: 97-106. Go to original source...
    22. Sharkey T.D. (2005): Effects of moderate heat stress on photosyntesis: importance of thylakoid reactions, rubisco deactivation, reactive oxygen species, and thermotolerance provided by isoprene. Journal of Plant, Cell and Environment, 28: 269-277. Go to original source...
    23. Sharma D.K., Andersen S.B., Ottosen C.-O., Rosenqvist E. (2012): Phenotyping of wheat cultivars for heat tolerance using chlorophyll a fluorescence. Journal of Functional Plant Biology, 39: 936-947. Go to original source... Go to PubMed...
    24. Srivastava A., Guissé B., Greppin H., Strasser R.J. (1997): Regulation of antenna structure and electron transport in photosystem II of Pisum sativum under elevated temperature probed by the fast polyphasic chlorophyll a fluorescence transient: OKJIP. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1320: 95-106. Go to original source...
    25. Stirbet A., Lazár D., Kromdijk J., Govindjee G. (2018): Chlorophyll a fluorescence induction: can just a one-second measurement be used to quantify abiotic stress responses? Journal of Photosynthetica, 56: 86-104. Go to original source...
    26. Stirbet S.A., Govindjee G. (2011): On the relation between the Kautsky effect (chlorophyll a fluorescence induction) and photosystem II: basics and applications of the OJIP fluorescence transient. Journal of Photochemistry and Photobiology B: Biology, 104: 236-257. Go to original source... Go to PubMed...
    27. Strasser R.J., Srivastava A., Tsimilli-Michael M. (2000): The fluorescence transient as a tool to characterise and screen photosyntetic samples. Probing Photosynthesis Mechanisms, Regulation and Adaption, 25: 445-483.
    28. Tuberosa R. (2012): Phenotyping for drought tolerance of crops in the genomics era. Frontiers in Physiology, 3: 347. Go to original source... Go to PubMed...
    29. Wang W., Vinocur B., Altman A. (2003): Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance. Planta, 218: 1-14. Go to original source... Go to PubMed...
    30. Yang X., Chen X., Ge Q., Li B., Tong Y., Li Z., Kuang T., Lu C. (2007): Characterisation of photosynthesis of flag leaf in a wheat hybrid and its parents grown under field conditions. Journal of Plant Physiology, 164: 318-326. Go to original source... Go to PubMed...
    31. Yeh Ch.-H., Kaplinsky N.J., Hu C., Charng Y.-Y. (2012): Some like it hot, some like it warm: phenotyping to explore thermotolerance diversity. Journal of Plant Science, 195: 10-13. Go to original source... Go to PubMed...
    32. Zivcak M., Olsovksa K., Brestic M. (2017): Photosynthetic responses under harmful and changing environment: practical aspects in crop research. In: Hou H.J.M. (ed.): Photosynthesis: Structures, Mechanisms, and Applications. Dordrecht, Springer, 203-248. ISBN-13: 978-3319488714 Go to original source...
    33. Zivcak M. (2013): Photosynthetic electron transport and specific photoprotective responses in wheat leaves under drought stress. Photosynthesis Research, 117: 529-546. Go to original source... Go to PubMed...
    34. Zivcak M., Brestic M., Olsovska K., Slamka P. (2008): Performance index as a sensitive indicator of water stress in Triticum aestivum L. Plant, Soil and Environment, 54: 133-139. Go to original source...
    35. Zivcak M., Brestic M., Botyanszka L., Chen Y.-E., Allakhverdiev S.I. (2019): Phenotyping of isogenic chlorophyll-less bread and durum wheat mutant lines in relation to photoprotection and photosynthetic capacity. Photosynthesis Research, 139: 239-251. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content