Plant Soil Environ., 2023, 69(1):18-24 | DOI: 10.17221/286/2022-PSE

Adaptation analysis of insect-resistant transgenic line after introducing mcry1F gene in maizeOriginal Paper

Daming Wang1, Junqi Yin1, Fengci Wu1, Baifeng Wang1, Zhilei Jiang1, Jingang Liang2, Xinyuan Song1
1 Agro-Biotechnology Research Institute, Provincial Crop Transgenic Science and Technology Innovation Center, Jilin Academy of Agricultural Sciences, Changchun, P.R. China
2 Development Center of Science and Technology, Ministry of Agriculture and Rural Affairs, Beijing, P.R. China

The ability to adapt, survive, and compete with weeds of transgenic plants is the necessary evaluation content to release transgenic lines in target regions. We compared weediness and agronomic traits of transgenic maize lines G1F-8 and G1F-19 carrying the mcry1F gene with their near-isogenic maize inbred line Zheng 58 in the wasteland and cultivated field under natural conditions for two consecutive years. The results showed that there was no significant difference identified in the species, quantity, and relative coverage ratio (RCR) of weeds between fields with G1F-8, G1F-19, and Zheng 58, regardless of the sowing pattern in the wasteland. Compared with the vigour of weeds, none of G1F-8, G1F-19, and Zheng 58 showed survival advantages, and all showed weak growth potential with no final grain yield. Meanwhile, no volunteer seedlings were found upon investigation in the following year. The simulated seed overwintering experiment in the wasteland further showed that the three kinds of maize could not germinate in the second year. In cultivated land, G1F-8 and G1F-19 had the same growth stages, plant height, and RCR as Zheng 58 throughout two years. In conclusion, the transgenic lines G1F-8 and G1F-19 exhibited no adaptability risk in Gongzhuling, Jilin, China.

Keywords: Zea mays L.; transgenic technology; competition; ecological suitability; wilderness

Received: September 2, 2022; Accepted: January 20, 2023; Prepublished online: January 25, 2023; Published: January 29, 2023  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Wang D, Yin J, Wu F, Wang B, Jiang Z, Liang J, Song X. Adaptation analysis of insect-resistant transgenic line after introducing mcry1F gene in maize. Plant Soil Environ. 2023;69(1):18-24. doi: 10.17221/286/2022-PSE.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Berg J.V.D., Hilbeck A., Bøhn T. (2013): Pest resistance to Cry1Ab Bt maize: field resistance, contributing factors and lessons from South Africa. Crop Protection, 54: 154-160. Go to original source...
    2. Finger R., Benni N.E., Kaphengst T., Evans C., Herbert S., Lehmann B., Morse S., Stupak N. (2011): A meta analysis on farm-level costs and benefits of GM crops. Sustainability, 3: 743-762. Go to original source...
    3. Fu J.M., Song X.L., Liu B., Shi Y., Shen W.J., Fang Z.X., Zhang L. (2018): Fitness cost of transgenic cry1Ab/c rice under saline-alkaline soil condition. Frontiers in Plant Science, 9: 1552. Go to original source... Go to PubMed...
    4. Fu W., Zhu P.Y., Qu M.N., Zhi W., Zhang Y.J., Li F.W., Zhu S.F. (2021): Evaluation on reprogramed biological processes in transgenic maize varieties using transcriptomics and metabolomics. Scientific Reports, 11: 2050. Go to original source... Go to PubMed...
    5. Garcia-Alonso M., Hendley P., Bigler F., Mayeregger E., Parker R., Rubinstein C., Satorre E., Solari F., McLean M.A. (2014): Transportability of confined field trial data for environmental risk assessment of genetically engineered plants: a conceptual framework. Transgenic Research, 23: 1025-1041. Go to original source... Go to PubMed...
    6. Herman R.A., Song P., Zhuang M.B. (2011): Safety risks of cryptic reading frames and gene disruption due to crop transgenesis: what are the odds? GM Crops & Food: Biotechnology in Agriculture and the Food Chain, 2: 4-6. Go to original source... Go to PubMed...
    7. Huang Y., Guo R.Q., Liu B. (2017): Ecological adaptability of glyphosate-resistant transgenic soybean NZL06-698 in weed environment. Soybean Science, 36: 866-871. (In Chinese)
    8. Huang Y., Li J.K., Qiang S., Dai W.M., Song X.L. (2016): Transgenic restorer rice line T1c-19 with stacked cry1C*/bar genes has low weediness potential without selection pressure. Journal of Integrative Agriculture, 15: 1046-1058. Go to original source...
    9. ISAAA (2018): Global status of commercialized biotech/GM crops: 2017. China Biotechnology, 38: 1-8.
    10. ISAAA (2021): Global status of commercialized biotech/GM crops: 2019. China Biotechnology, 41: 114-119.
    11. Jiang Q.Y., Li X.H., Hu Z., Ma Y.Z., Zhang H., Xv Z.S. (2015): Safety assessment of weediness of transgenic drought-tolerant wheat (Triticum aestivum L.). Scientia Agricultura Sinica, 48: 2096-2107. (In Chinese)
    12. Lee M.S., Anderson E.K., Stojšin D., McPherson M.A., Baltazar B., Horak M.J., de la Fuente J.M., Wu K.S., Crowley J.H., Rayburn A.L., Lee D.K. (2017): Assessment of the potential for gene flow from transgenic maize (Zea mays L.) to eastern gamagrass (Tripsacum dactyloides L.). Transgenic Research, 26: 501-514. Go to original source... Go to PubMed...
    13. Liu Y.B., Darmency H., Stewart C.N.Jr., Wei W., Tang Z.X., Ma K.P. (2015): The effect of Bt-transgene introgression on plant growth and reproduction in wild Brassica juncea. Transgenic Research, 24: 537-547. Go to original source... Go to PubMed...
    14. Lu B.R. (2014): Assessing transgene escape and its environmental biosafety impacts: a case study in insect-resistant transgenic rice. Journal of Biosafety, 23: 217-223.
    15. Marral M.W.R., Khan M.B., Ahmad F., Farooq S., Hussain M. (2020): The influence of transgenic (Bt) and non-transgenic (non-Bt) cotton mulches on weed dynamics, soil properties and productivity of different winter crops. PLoS One, 25: e0238716. Go to original source... Go to PubMed...
    16. Raybould A., Higgins L.S., Horak M.J., Layton R.J., Storer N.P., De La Fuente J.M., Herman R.A. (2012): Assessing the ecological risks from the persistence and spread of feral populations of insect-resistant transgenic maize. Transgenic Research, 21: 655-664. Go to original source... Go to PubMed...
    17. Raybould A., Tuttle A., Shore S., Stone T. (2011): Environmental risk assessments for transgenic crops producing output trait enzymes. Transgenic Research, 21: 655-664. Go to original source... Go to PubMed...
    18. Sammons B., Whitsel J., Stork L.G., Reeves W., Horak M. (2014): Characterization of drought-tolerant maize MON 87460 for use in environmental risk assessment. Crop Science, 54: 719-729. Go to original source...
    19. Santos-Amaya O.F., Tavares C.S., Monteiro H.M., Teixeira T.P.M., Guedes R.N.C., Alves P.A., Pereira E.J.G. (2016): Genetic basis of Cry1F resistance in two Brazilian populations of fall armyworm, Spodoptera frugiperda. Crop Protection, 81: 154-162. Go to original source...
    20. Wan P., Huang Y.X., Wu H.H., Huang M.S., Cong S.B., Tabashnik B.E., Wu K.M. (2012): Increased frequency of pink bollworm resistance to Bt toxin Cry1Ac in China. PLoS One, 7: e29975. Go to original source... Go to PubMed...
    21. Wang D.M., Zhou L., Song X.Y. (2016): Evaluated of transgenic herbicide-resistant maize CL38-1 for glyphosate resistance and weed risk assessment. Journal of Weed Science, 36: 23-28. (In Chinese)
    22. Xv L.N., Zhou Z.Y., Hu F., He K.L., Hu B.J. (2018): Expression of miR-31 in Asian corn borer (Ostrinia furnacalis) and its relationship with Cry1Ab protein sensitivity. Journal of Agricultural Biotechnology, 26: 1962-1969. (In Chinese)
    23. Yang Y., Chen X.P., Cheng L.S., Cao F.Q., Romeis J., Li Y.H., Peng Y.F. (2015): Toxicological and biochemical analyses demonstrate no toxic effect of Cry1C and Cry2A to Folsomia candida. Scientific Reports, 5: 15619. Go to original source...
    24. Yang Y., Liu Y., Cao F.Q., Chen X.P., Cheng L.S., Romeis J., Li Y.H., Peng Y.F. (2014): Consumption of Bt rice pollen containing Cry1C or Cry2A protein poses a low to negligible risk to the silkworm Bombyx mori (Lepidoptera: Bombyxidae). PLoS One, 9: e102302. Go to original source... Go to PubMed...
    25. Yang Y., Zhang B., Zhou X., Romeis J., Peng Y.F., Li Y.H. (2018): Toxicological and biochemical analyses demonstrate the absence of lethal or sublethal effects of cry1C- or cry2A-expressing Bt rice on the Collembolan Folsomia candida. Frontiers in Plant Science, 9: 131. Go to original source... Go to PubMed...
    26. Zhang H.N., Yin W., Zhao J., Jin L., Yang Y.H., Wu S.W., Tabashnik B.E., Wu Y.D. (2011): Early warning of cotton bollworm resistance associated with intensive planting of Bt cotton in China. PLoS One, 6: e22874. Go to original source... Go to PubMed...
    27. Zhang J.J., Wu X.Y., Cao Y.P. (2017): Study on herbicide-resistance and survival competitiveness in cultivated land of transgenic glyphosate-resistant soybean. Journal of Shanghai Jiaotong University (Agricultural Science), 35: 26-30. (In Chinese)

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content