Plant Soil Environ., 2023, 69(4):170-178 | DOI: 10.17221/42/2023-PSE

Heavy metals content and health risk assessment of selected leafy plants consumed in Bosnia and HerzegovinaOriginal Paper

Mirha Pazalja1, Jasmina Sulejmanović2, Sabina Begić2, Mirsada Salihović1
1 University of Sarajevo, Faculty of Pharmacy, Sarajevo, Bosnia and Herzegovina
2 University of Sarajevo, Faculty of Science, Sarajevo, Bosnia and Herzegovina

Today, there is widespread concern about the potential health effects on populations from consuming contaminated leafy plants and vegetables. In this study, heavy metal content is present in commonly consumed leafy plants (Atriplex hortensis, Spinacia oleracea, Urtica dioica, Beta vulgaris, and Brassica oleracea) from the mining area near Tuzla in Bosnia and Herzegovina was determined. After the preparation of the samples by wet digestion with HNO3, the flame and graphite furnace atomic absorption spectrometry was used. According to the results, the lowest concentration in leafy plants was obtained for Cr 0.08 mg/kg (Brassica oleracea) and the highest for Fe 539.15 mg/kg (Spinacia oleracea). The novelty of this study was to estimate health risk assessment for selected leafy plants. The estimated daily intake (EDI) of Pb, Mn, Zn, and Cd from consuming leafy plants was higher than the maximum tolerated daily intake. For adults, the total target hazard quotient (THQ) calculated based on EDI of the heavy metals was found to be > 1 for Pb and Cd due to all leafy plant consumption and for the children risk level of THQ was observed for most heavy metals. The hazard index due to the intake of toxic metals from ingesting leafy plants was much > 1. According to the total carcinogenic risk index for adults and children, the carcinogenic risks for all samples were higher than the tolerable range. Based on the results of this study, there is a significant non-carcinogenic and carcinogenic health risk to the population associated with the consumption of leafy plants cultivated in the mining area.

Keywords: hazard elements; non-carcinogenic and carcinogenic analysis; food safety

Received: January 30, 2023; Revised: April 1, 2023; Accepted: April 3, 2023; Prepublished online: April 21, 2023; Published: April 25, 2023  Show citation

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Pazalja M, Sulejmanović J, Begić S, Salihović M. Heavy metals content and health risk assessment of selected leafy plants consumed in Bosnia and Herzegovina. Plant Soil Environ. 2023;69(4):170-178. doi: 10.17221/42/2023-PSE.
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    References

    1. Ahmed S., Mahdi M.M., Nurnabi M., Alam M.Z., Choudhury T.R. (2022): Health risk assessment for heavy metal accumulation in leafy vegetables grown on tannery effluent contaminated soil. Toxicology Reports, 9: 346-355. Go to original source... Go to PubMed...
    2. Aloud S.S., Alotaibi K.D., Almutairi K.F., Albarakah F.N. (2022): Assessment of heavy metals accumulation in soil and native plants in an industrial environment, Saudi Arabia. Sustainability, 14: 5993. Go to original source...
    3. Barboiu G., Radulescu C., Popescu I., Dulama I., Bucurică I., Teodorescu S., Stirbescu R.M., Tanase N. (2020): Potential health risk assessment associated with heavy metal accumulation in native Urtica dioica. Romanian Reports in Physics, 72: 711.
    4. Bislimi K., Halili J., Sahiti H., Bici M., Mazreku I. (2021): Effect of mining activity in accumulation of heavy metals in soil and plant (Urtica dioica L). Journal of Ecological Engineering, 22: 1-7. Go to original source...
    5. Briffa J., Sinagra E., Blundell R. (2020): Heavy environmental metal pollution and their toxicological effects on humans. Heliyon, 6: e04691. Go to original source... Go to PubMed...
    6. Cai L.M., Xu Z.C., Qi J.Y., Feng Z.Z., Xiang T.S. (2015): Assessment of exposure to heavy metals and health risks among residents near Tonglushan mine in Hubei, China. Chemosphere, 127: 127-135. Go to original source... Go to PubMed...
    7. Devi C.M., Swapna T.S. (2022): Weed plants: a boon for remediation of heavy metal contaminated soil. In: Kumar V., Shah M., Shahi S. (eds.): Phytoremediation Technology for the Removal of Heavy Metals and Other Contaminants from Soil and Water. Amsterdam, Elsevier, 127-141. ISBN: 9780323885485 Go to original source...
    8. Domac R. (1994): Flora of Croatia: Manual for Identification of Plants. Školska knjiga. Zagreb, 294-296.
    9. Engwa G.A., Ferdinand P.U., Nwalo F.N., Unachukwu M.N. (2019): Mechanism and health effects of heavy metal toxicity in humans. Poisoning in the Modern World-New Tricks for an Old Dog, 10: 70-90.
    10. European Union (2006): Commission Regulation (EC) No. 1881/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs. Offi-cial Journal of the European Union, 364: 5-24. Available at: https://eur-lex.europa.eu/eli/reg/2021/1317/oj
    11. Gebeyehu H.R., Bayissa L.D. (2020): Levels of heavy metals in soil and vegetables and associated health risks in Mojo area, Ethiopia. PloS One, 15: e0227883. Go to original source... Go to PubMed...
    12. Hayek A. (1927): Prodromus florae peninsulae Balcanicae. Repertorium Specierum Novarum Regni Vegetabilis, 30: 1-472.
    13. Huremović J., Horvat M., Kotnik J., Kocman D., Žižek S., Ribeiro Guevara S., Muhić-Šarac T., Memić M. (2017): Characterization of mercury contamination surrounding a chloralkali production facility in Tuzla, Bosnia and Herzegovina. Analytical Letters, 50: 1049-1064. Go to original source...
    14. Husejnović M.Š., Janković S., Nikolić D., Antonijević B. (2021): Human health risk assessment of lead, cadmium, and mercury co-exposure from agricultural soils in the Tuzla Canton (Bosnia and Herzegovina). Archives of Industrial Hygiene and Toxicology, 72: 268-279. Go to original source... Go to PubMed...
    15. IBM Corp. Released (2016): IBM SPSS Statistics for Windows Version 24.0. IBM Corp, Armonk.
    16. Javorka S., Csapody V. (1991): Iconographia Florae Partis Austro-Orientalis Europae Centralis. Budapest, Akadémiai Kiadó (Reprint), 576. (In Latin)
    17. Kowalska G. (2021): The safety assessment of toxic metals in commonly used herbs, spices, tea, and coffee in Poland. International Journal of Environmental Research and Public Health, 18: 5779. Go to original source... Go to PubMed...
    18. Lăcătușu R. (2014): Contributions regarding heavy metals flow within soil-plant-animal system in polluted areas. Acta Metallomica-MEEMB, 1: 73-88.
    19. Liu X.M., Gu S.B., Yang S.Y., Deng J.S., Xu J.M. (2021): Heavy metals in soil-vegetable system around E-waste site and the health risk assessment. Science of The Total Environment, 779: 146438. Go to original source... Go to PubMed...
    20. Mahmood A., Malik R.N. (2014): Human health risk assessment of heavy metals via consumption of contaminated vegetables collected from different irrigation sources in Lahore, Pakistan. Arabian Journal of Chemistry, 7: 91-99. Go to original source...
    21. Manea D.N., Ienciu A.A., Ştef R., Şmuleac I.L., Gergen I.I., Nica D.V. (2020): Health risk assessment of dietary heavy metals intake from fruits and vegetables grown in selected old mining areas - a case study: the Banat Area of Southern Carpathians. International Journal of Environmental Research and Public Health, 17: 5172. Go to original source... Go to PubMed...
    22. Mateos-Maces L., Chávez-Servia J.L., Vera-Guzmán A.M., Aquino-Bolaños E.N., Alba-Jiménez J.E., Villagómez-González B.B. (2020): Edible leafy plants from Mexico as sources of antioxidant compounds, and their nutritional, nutraceutical and antimicrobial potential: a review. Antioxidants, 9: 541. Go to original source... Go to PubMed...
    23. Murtić S., Zahirović Ć., Čivić H., Sijahović E., Jurković J., Avdić J., Šahinović E., Podrug A. (2021): Phytoaccumulation of heavy metals in native plants growing on soils in the Spreča river valley, Bosnia and Herzegovina. Plant, Soil and Environment, 67: 533-540. Go to original source...
    24. Musilová J., Harangozo Ľ., Franková H., Lidiková J., Vollmannová A., Tóth T. (2021): Hygienic quality of soil in the Gemer region (Slovakia) and the impact of risk elements contamination on cultivated agricultural products. Scientific Reports, 11: 14089. Go to original source... Go to PubMed...
    25. Musilová J., Franková H., Lidiková J., Chlpík J., Vollmannová A., Árvay J., Harangozo L., Urminská J., Tóth T. (2022): Impact of old environmental burden in the Spiš region (Slovakia) on soil and home-grown vegetable contamination, and health effects of heavy metals. Scientific Reports, 12: 16371. Go to original source... Go to PubMed...
    26. Nica D.V., Bordean D.M., Pet I., Pet E., Alda S., Gergen I. (2013): A novel exploratory chemometric approach to environmental monitorring by combining block clustering with Partial Least Square (PLS) analysis. Chemistry Central Journal, 7: 1-10. Go to original source... Go to PubMed...
    27. Okereafor U., Makhatha M., Mekuto L., Uche-Okereafor N., Sebola T., Mavumengwana V. (2020): Toxic metal implications on agricultural soils, plants, animals, aquatic life and human health. International Journal of Environmental Research and Public Health, 17: 2204. Go to original source... Go to PubMed...
    28. Pazalja M., Salihović M., Smajović A. (2022): Operator exposure to heavy metals from wood pellet ash-risk assessment. Journal of Chemists and Chemical Engi-neers, 71: 551-556. Go to original source...
    29. Prisacaru A.E., Apostol L.C., Ropciuc S. (2017): Estimation of heavy metal levels in green leafy vegetables purchased from Suceava. Food and Environment Safety Journal, 16: 234-238.
    30. Proshad R., Kormoker T., Islam Md.S., Chandra K. (2020): Potential health risk of heavy metals via consumption of rice and vegetables grown in the industrial areas of Bangladesh. Human and Ecological Risk Assessment: an International Journal, 26: 921-943. Go to original source...
    31. Saleem M.H., Ali S., Rehman M., Hasanuzzaman M., Rizwan M., Irshad S., Shafiq F., Iqbal M., Alharbi B.M., Alnusaire T.S., Qari S.H. (2020): Jute: a potential candidate for phytoremediation of metals - a review. Plants, 9: 258. Go to original source... Go to PubMed...
    32. Salihović M., Pazalja M., Šapčanin A., Dojčinović B.P., Špirtović-Halilović S. (2021): Element contents and health risk assessment in wild edible mushrooms of Bosnia and Herzegovina. Plant, Soil and Environment, 67: 668-677. Go to original source...
    33. Saraiva M.A., Chekri R., Guérin T., Sloth J.J., Jitaru P. (2021): Chromium speciation analysis in raw and cooked milk and meat samples by species-specific isotope dilution and HPLC-ICP-MS. Food Additives and Contaminants: Part A, 38: 304-314. Go to original source... Go to PubMed...
    34. Sarkar T., Salauddin M., Roy S., Chakraborty R., Rebezov M., Shariati M.A., Thiruvengadam M., Rengasamy K.R.R. (2022): Underutilized green leafy vegetables: frontier in fortified food development and nutrition. Critical Reviews in Food Science and Nutrition, 2022: 1-55. Go to original source... Go to PubMed...
    35. Sultana M.S., Rana S., Yamazaki S., Aono T., Yoshida S., Kanan S. (2017): Health risk assessment for carcinogenic and non-carcinogenic heavy metal exposures from vegetables and fruits of Bangladesh. Cogent Environmental Science, 3: 1291107. Go to original source...
    36. Tóth G., Hermann T., Da Silva M.R., Montanarella L.J.E.I. (2016): Heavy metals in agricultural soils of the European Union with implications for food safety. Envi-ronment International, 88: 299-309. Go to original source... Go to PubMed...
    37. Trinajstić I. (ed.) (1975-1986): Analytical Flora of Yugoslavia 2 (1-4). University of Zagreb, University Publishing House Liber.
    38. USEPA (2011): Exposure Factors Handbook. Final edition. EPA/600/R-09/052F,Washington, DC. Available at: https://cfpub.epa.gov/ncea/risk/recordisplay.cfm?deid=236252 (accessed on 12. 11. 2022)
    39. USEPA (2012): Integrated Risk Information System of the US Environmental Protection Agency. Washington, U.S. Environmental Protection Agency Available at: https://www.epa.gov/iris (accessed on 12. 12. 2022)
    40. USEPA (2017): United States Environmental Protection Agency. IRIS Assessments. Washington, U.S. Environmental Protection Agency Available at: https://cfpub.epa.gov/ncea/iris2/atoz.cfm (accessed on 19. 12. 2022)
    41. Zhang H.Y., Yuan X.Z., Xiong T., Wang H., Jiang L.B. (2020): Bioremediation of co-contaminated soil with heavy metals and pesticides: influence factors, mecha-nisms and evaluation methods. Chemical Engineering Journal, 398: 125657. Go to original source...

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