Plant Soil Environ., 2023, 69(10):463-470 | DOI: 10.17221/225/2023-PSE

Inoculation with Bacillus alters nitrogen uptake and metabolism in roots of Diospyros lotus under wheat straw addition in soilOriginal Paper

Peng Zhang1,3, Fengge Hao1,3, Zitan Zhu2, Dongmei Lang1,3, Huiling Hu1,3
1 School of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang, Henan, P.R. China
2 Liaoning Institute of Saline-alkali Land Utilization, Panjin, Liaoning, P.R. China
3 Henan Province Engineering Research Center of Horticultural Plant Resource Utilization and Germplasm Enhancement, Xinxiang, Henan, P.R. China

A 15N-labelling technique was carried out to investigate the effect of wheat straw co-application with Bacillus nealsonii, Cohnella, and Paenibacillus lautus on N uptake and assimilation in Diospyros lotus. Wheat straw combined with Bacillus increased the plant height, biomass accumulation, photosynthetic capacity, and uptake of 15N by roots, with 15N accumulating mainly in leaves of D. lotus. The NO3–-N content in roots and leaves were decreased by wheat straw co-application with Bacillus, whereas NH4+-N, soluble protein, and total N contents were increased. Wheat straw addition promoted the activities of nitrate reductase, glutamine synthase and glutamate synthase in roots rather than in leaves. These N assimilation enzymatic activities, and glutamic-oxaloacetic transaminase and glutamic pyruvic transaminase activities were markedly increased by wheat straw combined with Bacillus. Moreover, the combined application of wheat straw and Bacillus, particularly B. nealsonii, improved the N use efficiency. These findings suggest that the combined application of wheat straw and Bacillus improved D. lotus growth by increasing N uptake, metabolism, and utilisation efficiency.

Keywords: 15N-uptake; nitrogen assimilation; persimmon tree; nutrition; isotope

Received: June 3, 2023; Revised: September 27, 2023; Accepted: October 2, 2023; Prepublished online: October 25, 2023; Published: October 30, 2023  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Zhang P, Hao F, Zhu Z, Lang D, Hu H. Inoculation with Bacillus alters nitrogen uptake and metabolism in roots of Diospyros lotus under wheat straw addition in soil. Plant Soil Environ. 2023;69(10):463-470. doi: 10.17221/225/2023-PSE.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Aquino J.P.A., Antunes J.E.L., Bonifácio A., Rocha S.M.B., Amorim M.R., Neto F.A., Araujo A.S.F. (2021): Plant growth-promoting bacteria im-prove growth and nitrogen metabolism in maize and sorghum. Theoretical and Experimental Plant Physiology, 33: 249-260. Go to original source...
    2. Baslam M., Mitsui T., Sueyoshi K., Ohyama T. (2023): Recent advances in carbon and nitrogen metabolism in C3 plants. International Journal of Molecular Sciences, 22: 318. Go to original source... Go to PubMed...
    3. Beadle C.L., Long S.P. (1985): Photosynthesis - is it limiting to biomass production? Biomass, 8: 119-168. Go to original source...
    4. Bradford M.M. (1976): A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72: 248-254. Go to original source...
    5. Cataldo D.A., Maroon M., Schrader L.E., Youngs V.L. (1975): Rapid colorimetric determination of nitrate in plant tissue by nitration of salicylic acid. Communications in Soil Science and Plant Analysis, 6: 71-80. Go to original source...
    6. Datta R., Sharma R. (1999): Temporal and spatial regulation of nitrate reductase and nitrite reductase in greening maize leaves. Plant Science, 144: 77-83. Go to original source...
    7. Du T.Y., He H.H., Zhang Q., Lu L., Mao W.J., Zhai M.Z. (2022): Positive effects of organic fertilizers and biofertilizers on soil microbial community composition and walnut yield. Applied Soil Ecology, 175: 104457. Go to original source...
    8. Gomez-Ramirez L.F., Uribe-Velez D. (2021): Phosphorus solubilizing and mineralizing Bacillus spp. contribute to rice growth promotion using soil amended with rice straw. Current Microbiology, 78: 932-943. Go to original source... Go to PubMed...
    9. Guo R.Y., Miao W., Fan C.G., Li X.G., Shi Y.Y., Li F.M., Qin W. (2021): Exploring optimal nitrogen management for high yielding maize in arid areas via 15N-labeled technique. Geoderma, 382: 114711. Go to original source...
    10. Hodges M. (2002): Enzyme redundancy and the importance of 2-oxoglutarate in plant ammonium assimilation. Journal of Experimental Botany, 53: 905-916. Go to original source... Go to PubMed...
    11. Jourgholami M., Fathi K., Labelle E.R. (2020): Effects of litter and straw mulch amendments on compacted soil properties and Caucasian alder (Alnus subcordata) growth. New Forests, 51: 349-365. Go to original source...
    12. Krom M.D. (1980): Spectrophotometric determination of ammonia: a study of a modified Berthelot reaction using salicylate and dichloroisocy-anurate. Analyst, 105: 305-316. Go to original source...
    13. Lea P.J., Leegood R.C. (1999): Plant Biochemistry and Molecular Biology. 2nd Edition. Chichester, John Wiley.
    14. Lichtenthaler H.K., Wellburn A.R. (1983): Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Bio-chemical Society Transactions, 11: 591-592. Go to original source...
    15. Luo Z., Wang R. (2008): Persimmon in China: domestication and traditional utilizations of genetic resources. Advances in Horticultural Science, 22: 1000-1005.
    16. Masood S., Zhao X.Q., Shen R.F. (2020): Bacillus pumilus promotes the growth and nitrogen uptake of tomato plants under nitrogen fertilization. Scientia Horticulturae, 272: 109581. Go to original source...
    17. Poveda J., González-Andrés F. (2021): Bacillus as a source of phytohormones for use in agriculture. Applied Microbiology and Biotechnology, 105: 8629-8645. Go to original source... Go to PubMed...
    18. Rennenberg H., Wildhagen H., Ehlting B. (2010): Nitrogen nutrition of poplar trees. Plant Biology, 12: 275-291. Go to original source... Go to PubMed...
    19. Solanki A.C., Gurjar N.S., Sharma S. (2023): Co-inoculation of non-symbiotic bacteria Bacillus and Paraburkholderia can improve the soybean yield, nutrient uptake, and soil parameters. Molecular Biotechnology. https://doi.org/10.1007/s12033-023-00719-w Go to original source... Go to PubMed...
    20. Tang Z.C. (1999): Experimental Guide of Modern Plant Physiology. Shanghai, Science Press.
    21. The S.V., Snyder R., Tegeder M. (2021): Targeting nitrogen metabolism and transport processes to improve plant nitrogen use efficiency. Fron-tiers in Plant Science, 11: 628366. Go to original source... Go to PubMed...
    22. Wang Z., Wang Z., Ma L.J., Lv X.B., Meng Y.L., Zhou Z.G. (2021): Straw returning coupled with nitrogen fertilization increases canopy photosyn-thetic capacity, yield and nitrogen use efficiency in cotton. European Journal of Agronomy, 126: 126267. Go to original source...
    23. Win K.T., Okazaki K., Ookawa T., Yokoyama T., Ohwaki Y. (2019): Influence of rice-husk biochar and Bacillus pumilus strain TUAT-1 on yield, biomass production, and nutrient uptake in two forage rice genotypes. Plos One, 14: e0220236. Go to original source... Go to PubMed...
    24. Yang K., Jiang Y.W., Wang J.N., Cai X.W., Wen Z., Qiu Z.L., Qiao G. (2022): Tobacco straw biochar improved the growth of Chinese cherry (Prunus pseudocerasus) via altering plant physiology and shifting the rhizosphere bacterial community. Scientia Horticulturae, 303: 111244. Go to original source...
    25. Zhang R.Q., Huang Q.Q., Yan T.Y., Yang J.F., Zheng Y., Li H.K., Li M. (2019a): Effects of intercropping mulch on the content and composition of soil dissolved organic matter in apple orchard on the loess plateau. Journal of Environmental Management, 250: 109531. Go to original source... Go to PubMed...
    26. Zhang X.Y., Liu X., Lan Z.Q., Tian Y.G., Li J.S. (2019b): Conjunctive use of composted leguminous shrub Caragana microphylla-straw and Bacillus cereus reduces nitrogen input but enhances nitrogen use efficiency and cucumber yields in alkaline soils. Applied Soil Ecology, 139: 69-78. Go to original source...
    27. Zhang Y.Q., Wang J.D., Gong S.H., Xu D., Mo Y. (2019c): Straw mulching enhanced the photosynthetic capacity of field maize by increasing the leaf N use efficiency. Agricultural Water Management, 218: 60-67. Go to original source...
    28. Zhu Y.Q., Lv X., Song J.H., Li W.D., Wang H.J. (2022): Application of cotton straw biochar and compound Bacillus biofertilizer decrease the bioavailability of soil Cd through impacting soil bacteria. BMC Microbiology, 22: 3. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content