Plant Soil Environ., 2023, 69(12):615-627 | DOI: 10.17221/306/2023-PSE

Polymorphism of Bolivian accessions of Arachis hypogaea L. revealed by allergen coding DNA markersOriginal Paper

Jana Žiarovská1, Lucia Urbanová2, Julio Montero-Torres3, Adam Kováčik1, Lucia Klongová2, Rohit Bharati4, Sandra Romero-Ortega3, Eloy Fernández-Cusimamani4, Olga Leuner4
1 Slovak University of Agriculture in Nitra, Faculty of Agrobiology and Food Resources, Institute of Plant and Environmental Sciences, Nitra, Slovak Republic
2 Slovak University of Agriculture in Nitra, Research Centre of AgroBioTech, Nitra, Slovak Republic
3 Major, Real and Pontifical University of San Francisco Xavier in Chuquisaca, Faculty of Agricultural Sciences, Germplasm Bank – BIORENA, Sucre, Bolivia
4 Czech University of Life Sciences Prague, Faculty of Tropical AgriSciences, Department of Crop Sciences and Agroforestry, Prague, Czech Republic

Arachis hypogaea L. is an annual legume that is one of the most consumed plant species. On the other hand, it belongs to one of the most monitored clinically important allergens worldwide. The polymorphism of this species based on allergen coding genes could be useful in its characterisation, but previously, no allergen-based marker techniques have been developed for peanuts. A new type of DNA-based markers of coding regions were used to analyse the variability of 21 peanut accessions – BBAP (Bet v1 based amplicon polymorphism), PBAP (profilin based amplicon polymorphism), and VBAP (vicilin based amplicon polymorphism). All of the used technique provided polymorphic fingerprints and distinguished the analysed peanut accessions. The effectivity of these techniques corresponds to the presence of the allergen homologous sequences that are a part of the A. hypogaea genome. VBAP was the most effective in distinguishing the analysed peanut accessions when compared to the results of BBAP and PBAB. For BBAP, two of the analysed accessions provided the same fingerprinting pattern. The ability of the used markers to detect polymorphisms was comparable, with an average polymorphism information content (PIC) value of 0.47.

Keywords: allergen markers; DNA analysis; food safety; genetic resources; peanut accessions

Received: July 27, 2023; Revised: November 14, 2023; Accepted: November 15, 2023; Prepublished online: December 17, 2023; Published: December 20, 2023  Show citation

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Žiarovská J, Urbanová L, Montero-Torres J, Kováčik A, Klongová L, Bharati R, et al.. Polymorphism of Bolivian accessions of Arachis hypogaea L. revealed by allergen coding DNA markers. Plant Soil Environ. 2023;69(12):615-627. doi: 10.17221/306/2023-PSE.
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