Plant Soil Environ., 2025, 71(1):58-65 | DOI: 10.17221/586/2024-PSE

Ability of soil microorganisms to degrade aminopyralid and its effect on their growthOriginal Paper

Martin Koudela ORCID...1, Miroslava Soukupová ORCID...1, Eva Jablonská2, Tereza ©mrhová ORCID...2, Tomas Engl ORCID...2, Jaroslav Matějka3, Sebnem Kurhan3, Petr Marąík ORCID...3, David Novotný1, Čeněk Novotný ORCID...1,4
1 Department of Horticulture, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic
2 Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology in Prague, Prague, Czech Republic
3 Department of Food Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic
4 Laboratory of Environmental Biotechnology, Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic

The study aimed at the isolation of microorganisms from aminopyralid (AP)-contaminated soil, evaluation of their ability to degrade AP, and examination of the effects of AP on microbial growth. Geotrichum candidum, Cladosporium herbarum, Candida subhashii, and Paenarthrobacter nicotinovorans were isolated and identified using enrichment. None of those strains were able to degrade 100 ppm AP within 2–3 weeks. In contrast, collection strains Pleurotus ostreatus ‘Spoppo’ and Bacillus velezensis FZB42 reduced AP concentration by 35.1% and 47.8%, respectively. Low sensitivity of growth to AP (400 ppm) on the malt-extract-agar medium was observed; inhibition values for C. herbarum and G. candidum were 52.4% and 22.8%, respectively, compared to 33.7% inhibition found with P. ostreatus ‘Spoppo’. Promotion of fungal growth was observed at low AP concentrations in the Czapek-Dox medium, the highest effect being in G. candidum. The growth promotion effect was confirmed with P. ostreatus ‘Spoppo’growing on wheat straw contaminated with Mustang Forte and Corello herbicides; total fruiting body mass yield increased 1.25- and 1.37-fold, respectively. The study offers insight into future strategies for mitigating the environmental impact of synthetic auxin herbicides.

Keywords: soil microbiota; contamination; weed control; taxonomical identification; biodegradation capacity; liquid-chromatography mass-spectrometry analysis

Received: November 1, 2024; Revised: November 29, 2024; Accepted: December 2, 2024; Prepublished online: January 7, 2025; Published: January 8, 2025  Show citation

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Koudela M, Soukupová M, Jablonská E, ©mrhová T, Engl T, Matějka J, et al.. Ability of soil microorganisms to degrade aminopyralid and its effect on their growth. Plant Soil Environ. 2025;71(1):58-65. doi: 10.17221/586/2024-PSE.
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