Plant Soil Environ., 2025, 71(3):185-201 | DOI: 10.17221/530/2024-PSE

Irrigation methods and nitrogen-form interactions regulate starch-metabolising enzyme activity to improve rice yield and qualityOriginal Paper

Haojing Li1, Hairun Li1, Danke Zhang1, Mengmeng Jiang1, Jing Cao1, Guowei Xu1
1 College of Agriculture, Henan University of Science and Technology, Luoyang, Henan, P.R. China

Nitrogen management and irrigation methods play crucial roles in determining rice’s grain yield and quality (Oryza sativa L.). However, limited knowledge exists on how interactions between nitrogen forms and irrigation regimes regulate starch-metabolising enzyme activity to influence rice yield and quality. A soil-growth experiment was conducted using a high-lodging-resistance rice cultivar under three irrigation methods, namely, submerged irrigation (0 kPa), alternate wetting and moderate drying (−20 kPa), and alternate wetting and severe drying (−40 kPa), as well as three nitrogen forms, namely, ammonium nitrogen (NH4+-N), mixed ammonium + nitrate (50 : 50), hereafter denoted as 50 : 50, and nitrate nitrogen (NO3–-N). Results indicated that compared with the other treatments, alternate wetting and moderate drying interacted with 50 : 50 treatment, resulting in the following: improved grain yield by 11.7–21.0%, milling, appearance, eating and cooking, and nutritional qualities including milled-rice and gel consistency; and decreased chalky rice, chalky size, chalky degree, amylose content, and protein content by 20.0–23.1, 29.6–33.3, 44.1–48.5, 6.2–9.6 and 10.1–13.9%, respectively. The activities of adenosine phosphate glucose pyrophosphorylase (AGPase), starch synthase (SS), starch-branching enzyme (SBE), and adenosine triphosphate (ATP) enzyme in the grains also improved, with an increase of 20.0–35.0, 11.8–20.0, 13.6–26.3 and 21.2–39.6%, respectively. Conversely, severe drying and NO3–-N treatment negatively impacted grain yield and quality due primarily to decreased SS activity in grains under each irrigation method. Correlation analysis showed that starch-metabolising enzyme (AGPase, SS and SBE) activity at 14 days after anthesis (DAA) and 28 DAA exhibited a positive correlation with grain yield, milling quality and gel consistency, whereas negatively correlated with appearance and nutritional qualities. In summary, the adoption of alternate wetting and moderate drying and 50 : 50 interaction treatment can synergistically boost grain yield by increasing the filled-grain rate and 1 000-grain weight and enhance grain quality of rice by upregulating the activities of starch-metabolising enzyme activity.

Keywords: rice (Oryza sativa L.); production; water management; water stress; nutrition; quality formation; starch synthesis

Received: October 3, 2024; Revised: March 2, 2025; Accepted: March 10, 2025; Prepublished online: March 14, 2025; Published: March 31, 2025  Show citation

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Li H, Li H, Zhang D, Jiang M, Cao J, Xu G. Irrigation methods and nitrogen-form interactions regulate starch-metabolising enzyme activity to improve rice yield and quality. Plant Soil Environ. 2025;71(3):185-201. doi: 10.17221/530/2024-PSE.
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    References

    1. Ali L., Ullah S., Iqbal A., Quan Z., Liang H., Ahmad S., Muhammad I., Amanullah I., Guo Z.X., Wei S.Q. (2021): Combined application of biochar and nitrogen fertilizer promotes the activity of starch metabolism enzymes and the expression of related genes in rice in a dual cropping system. BMC Plant Biology, 21: 1-15. Go to original source... Go to PubMed...
    2. Abu-Ali L., Maguffin S.C., Rohila J.S., McLung A.M., Reid M.C. (2023): Effects of alternate wetting and drying on oxyanion-forming and cationic trace elements in rice paddy soils: impacts on arsenic, cadmium, and micronutrients in rice. Environmental Geochemistry and Health, 45: 8135-8151. Go to original source... Go to PubMed...
    3. Aye M.A., Thu C.N., Htwe N.M. (2020): Source-sink relationship and their influence on yield performance of YAU promising rice lines. Journal of Experimental Agriculture International, 42: 124-135. Go to original source...
    4. Carrijo D.R., Akbar N., Reis A.F.B., Li C.Y., Gaudin A.C.M., Parikh S.J., Green P.G., Linquist B.A. (2018): Impacts of variable soil drying in alternate wetting and drying rice systems on yields, grain arsenic concentration and soil moisture dynamics. Field Crops Research, 222: 101-110. Go to original source...
    5. Carrijo D.R., Lundy M.E., Linquist B.A. (2017): Rice yields and water use under alternate wetting and drying irrigation: a meta-analysis. Field Crops Research, 203: 173-180. Go to original source...
    6. Chen B.P., Guo L.J., Tang J.C., Li Y.S., Li C.F. (2024a): Comprehensive impacts of different integrated rice-animal co-culture systems on rice yield, nitrogen fertilizer partial factor productivity and nitrogen losses: a global meta-analysis. Science of the Total Environment, 915: 169994. Go to original source... Go to PubMed...
    7. Chen R.R., Li D.X., Fu J., Qin P., Zhang X.W., Sun Z.B., He K., Li L., Zhou W., Wang Y.J., Wang K., Liu X.M., Yang Y.Z. (2024b): Exploration of quality variation and stability of hybrid rice under multi-environments. Molecular Breeding, 44: 4. Go to original source... Go to PubMed...
    8. Dou Z., Zhou Y.C., Zhang Y.Y., Guo W., Xu Q., Gao H. (2024): Influence of nitrogen applications during grain-filling stage on rice (Oryza sativa L.) yield and grain quality under high temperature. Agronomy, 14: 216. Go to original source...
    9. Fei L.W., Pan Y.H., Ma H.L., Guo R.J., Wang M., Ling N., Shen Q.R., Guo S.W. (2024): Optimal organic-inorganic fertilization increases rice yield through source-sink balance during grain filling. Field Crops Research, 308: 109285. Go to original source...
    10. Fu H.Y., Cui D.D., Shen H. (2021): Effects of nitrogen forms and application rates on nitrogen uptake, photosynthetic characteristics and yield of double-cropping rice in south China. Agronomy, 11: 158. Go to original source...
    11. Gajda A.M., Czyz E.A., Stanek-Tarkowska J., Dexter A.R., Furtak K.M., Grzadziel J. (2017): Effects of long-term tillage practices on the quality of soil under winter wheat. Plant, Soil and Environment, 63: 236-242. Go to original source...
    12. González A.F., Fang Z.T., Sen D., Henrich B., Nagashima Y., Sokolov A.V., Okumoto S., Verhoef A.J. (2024): In-vivo Raman microspectros-copy reveals differential nitrate concentration in different developmental zones in Arabidopsis roots. Plant Methods, 20: 185. Go to original source... Go to PubMed...
    13. Guo J.X., Jia Y.M., Chen H.H., Zhang L.J., Yang J.C., Zhang J., Hu X.Y., Ye X., Li Y., Zhou Y. (2019): Growth, photosynthesis, and nutrient uptake in wheat are affected by differences in nitrogen levels and forms and potassium supply. Scientific Report, 9: 1248. Go to original source... Go to PubMed...
    14. Hamoud Y.A., Shaghaleh H., Sheteiwy M., Guo X.P., Elshaikha N.A., Khan N.U., Oumarou A., Rahim S.F. (2019): Impact of alternative wetting and soil drying and soil clay content on the morphological and physiological traits of rice roots and their relationships to yield and nutrient use-efficiency. Agricultural Water Management, 223: 105706. Go to original source...
    15. Hou D.P., Liu K., Liu S.K., Li J.C., Tan J.S., Bi Q.Y., Zhang A.N., Yu X.Q., Bi J.G., Luo L.J. (2024): Enhancing root physiology for increased yield in water-saving and drought-resistance rice with optimal irrigation and nitrogen. Frontiers in Plant Science, 15: 1370297. Go to original source... Go to PubMed...
    16. Iqbal A., Xie H.M., He L., Ahmad S., Hussain I., Raza H., Khan A., Wei S.Q., Zhao Q., Wu K., Ali I., Jiang L.G. (2021): Partial substitution of organic nitrogen with synthetic nitrogen improves rice yield, grain starch metabolism and related gene expression under the dual cropping system. Saudi Journal of Biological Sciences, 28: 1283-1296. Go to original source... Go to PubMed...
    17. Ishfaq M., Akbar N., Anjum S.A., Anwar-Ul-haq M. (2020): Growth, yield and water productivity of dry direct seeded and transplanted aromatic rice under different irrigation management regimes. Journal of Integrative. Agriculture, 19: 2656-2673. Go to original source...
    18. Ishfaq M., Akbar N., Zulgiqar U., Ali N., Ahmad M., Anjum S.A., Farooq M. (2021): Influence of water management techniques on milling recovery, grain quality and mercury uptake in different rice production systems. Agricultural Water Management, 243: 106500. Go to original source...
    19. Jiang Y., Tao W.L., Zhang W.Y., Wang Z.Q., Yang J.C. (2024): Wetting alternating with partial drying during grain filling increases lysine biosynthesis in inferior rice grain. The Crop Journal, 12: 262-270. Go to original source...
    20. Ju C.X., Liu T., Sun C.M. (2021): Effect of controlled soil drying on the grain yield and nutritional quality of rice. Crop Science, 61: 1426-1435. Go to original source...
    21. Li G.H., Hu Q.Q., Shi Y.G., Cui K.H., Nie L.X., Huang J.L., Peng S.B. (2018): Low nitrogen application enhances starch-metabolizing enzyme activity and improves accumulation and translocation of non-structural carbohydrates in rice stems. Frontiers in Plant Science, 9: 1128. Go to original source... Go to PubMed...
    22. Ma Z.T., Chen X., Cao J.L., Yu J.H., Zhu Y., Liu G.D., Xu F.F., Hu Q., Zhang H.C., Li G.Y., Wei H.Y. (2025): Carbon and nitrogen metabolism effects on eating quality in grains of diverse Japonica rice cultivars from the middle and lower Yangtze river. Food and Energy Security, 14: e70051. Go to original source...
    23. Maneepitak S., Ullah H., Paothong K., Kachenchart B., Datta A., Shrestha R.P. (2019): Effect of water and rice straw management practices on yield and water productivity of irrigated lowland rice in the central plain of Thailand. Agricultural Water Management, 211: 89-97. Go to original source...
    24. Mi W.H., Hong Y., Gao F., Ma Y.Y., Sun T., Wu L.H., Wang G.Z., Chen S.T. (2024): Effect of a different form of N fertilization on yield sustainability and soil quality in double-cropped rice system in a long-term experiment. Journal of Soil Science and Plant Nutrition, 2: 2815-2824. Go to original source...
    25. Nakamura Y., Yuki K., Park S.Y. (1989): Carbohydrate metabolism in the developing endosperm of rice grains. Plant Cell Physiology, 30: 833-839. Go to original source...
    26. Pereira E.G., Santos L.A., Chapeta A.C., Ribeiro Y.D., Santa-Catarina C., Bucher C.P., Bucher C.A., Garcia A.C., Fernandes M.S. (2024): Disruption of amino acid transporter OsAAP1 impairs rice seedling establishment and nitrate uptake and assimilation. Journal of Plant Growth Regulation, 43: 2841-2854. Go to original source...
    27. Prasad R., Shivay Y.S., Kumar D. (2017): Current Status, Challenges, and Opportunities in Rice Production. Rice Production Worldwide. New York, Springer. Go to original source...
    28. Ranwala A.P., Miller W.B. (1998): Sucrose-cleaving enzymes and carbohydrate pools in Lilium longiflorum floral organs. Physiologia Planta-rum, 103: 541-550. Go to original source...
    29. Vicente L., Alvarenga P., Pena D., Fernandez D., Albarran A., Rato-Nunes J.M., Lopez-Pineiro A. (2025): Controlling As, Cd, and Pb bioaccumulation in rice under different levels of alternate wetting and drying irrigation with biochar amendment: a 3-year field study. Chemo-sphere, 372: 144114. Go to original source... Go to PubMed...
    30. Vuciterna R., Ruggeri G., Corsi S., Facchi A., Gharsallah D. (2024): A bibliometric analysis of scientific literature on alternate wetting and drying (AWD). Paddy and Water Management, 22: 415-430. Go to original source...
    31. Wand P., Wang Z.K., Sun X.C., Mu X.H., Chen H., Chen F.J., Ye L.X., Mi G.H. (2019): Interaction effect of nitrogen form and planting density on plant growth and nutrient uptake in maize seedlings. Journal of Integrative Agriculture, 18: 1120-1129. Go to original source...
    32. Wang Z.Q., Zhang W.Y., Beebout S.S., Zhang H., Liu L.J., Yang J.C., Zhang J.H. (2016): Grain yield, water and nitrogen use efficiencies of rice as influenced by irrigation regimes and their interaction with nitrogen rates. Field Crops Research, 193: 54-69. Go to original source...
    33. Wang J.Z., Zhang J.B., Zhao W., Müller C., Cai Z.C. (2017): Nitrification is the key process determining N use efficiency in paddy soils. Journal of Plant Nutrition and Soil Science, 180: 648-658. Go to original source...
    34. Wang X.P., Fu J.X., Min Z.S., Zou D.T., Liu H.L., Wang J.G., Zheng H.L., Jia Y., Yang L.M., Xin W., Sun B., Zhao H.W. (2022): Response of rice with overlapping growth stages to water stress by assimilates accumulation and transport and starch synthesis of superior and inferior grains. International Journal of Molecular Science, 23: 11157. Go to original source... Go to PubMed...
    35. Warwate S.I., Awana M., Thakare S.S., Krishnan V., Kumar S., Bollinedi H., Arora A., Sevanthi A.M., Ray M., Praveen S., Sinth A. (2024): Exploring the synergy of enzymes, nutrients, and gene networks in rice starch granule biogenesis. Frontiers in Nutrition, 11: 1448450. Go to original source... Go to PubMed...
    36. Wei C.H., Jiang J.J., Liu C., Fang X.C., Zhou T.Y., Xue Z.Y., Wang W.L., Zhang W.Y., Zhang H., Liu L.J., Wang Z.Q., Gu J.F., Yang J.C. (2023): Effects of source strength and sink size on starch metabolism, starch properties and grain quality of rice (Oryza sativa L.). Agronomy, 13: 1288. Go to original source...
    37. Wei X.D., Zhang Y.D., Song X.M., Zhao L., Zhao Q.Y., Chen T., Lu K., Zhu Z., Huang S.D., Wang C.L. (2024): Silicon and zinc fertilizer application improves grain quality and aroma in the japonica rice variety Nanjing 46. Foods, 13: 152. Go to original source... Go to PubMed...
    38. Xu G.W., Lu D.K., Wang H.Z., Li Y.J. (2018): Morphological and physiological traits of rice roots and their relationships to yield and nitrogen utilization as influenced by irrigation regime and nitrogen rate. Agricultural Water Management, 203: 385-394. Go to original source...
    39. Xu Y.J., Gu D.J., Li K., Zhang W.Y. (2019): Response of grain quality to alternate wetting and moderate soil drying irrigation in rice. Crop Science, 59: 1261. Go to original source...
    40. Xu G.W., Jiang M.M., Lu D.K., Wang H.Z., Chen M.C. (2020): Nitrogen forms affect the root characteristic, photosynthesis, grain yield, and nitrogen use efficiency of rice under different irrigation regimes. Crop Science, 60: 2594-2610. Go to original source...
    41. Xu G.W., Zhao X.H., Jiang M.M., Lu D.K., Chen M.C. (2021): Nitrogen forms and irrigation regimes interact to affect rice yield by regulating the source and sink characteristics. Agronomy Journal, 113: 4022-4036. Go to original source...
    42. Yang J.C., Zhang J.H., Wang Z.Q., Zhu Q.S., Liu L.J. (2003): Activities of enzymes involved in sucrose-to-starch metabolism in rice grains subjected to water stress during filling. Field Crops Research, 81: 69-81. Go to original source...
    43. Yang J.C., Zhang J.H., Wang Z.Q., Xu G.W., Zhu Q.S. (2004): Activities of key enzymes in sucrose-to-starch conversion in wheat grains subjected to water deficit during grain filling. Plant Physiology, 135: 1621-1629. Go to original source... Go to PubMed...
    44. Yang J.C., Zhou Q., Zhang J.H. (2017): Moderate wetting and drying increase rice yield and reduce water use, grain arsenic level, and methane emission. The Crop Journal, 5: 151-153. Go to original source...
    45. Yang X.X., Rasheed A., Yan H., Zhou C.H., Shang Q.Y. (2018): Effects of nitrogen forms and osmotic stress on root aerenchyma formation of japonica rice and its relationship with water absorption. International Journal of Agricultural Biology, 20: 2287-2292.
    46. Yi J., Gao J.P., Zhang W.Z., Zhao C., Wang Y., Zhen X.X. (2019): Differential uptake and utilization of two forms of nitrogen in japonica rice cultivars from north-eastern China. Frontier in Plant Science, 4: 1061. Go to original source... Go to PubMed...
    47. Yu T., Xin Y.N., Liu P. (2024): Exogenous abscisic acid (ABA) improves the filling process of maize grains at different ear positions by promoting starch accumulation and regulating hormone levels under high planting density. BMC Plant Biology, 24: 80. Go to original source... Go to PubMed...
    48. Zaghdoud C., Carvajal M., Ferchichi A., Martínez-Ballesta M. (2016): Water balance and N-metabolism in broccoli (Brassica oleracea L. var. Italica) plants depending on nitrogen source under salt stress and elevated CO2. Science of the Total Environment, 571: 763-771. Go to original source... Go to PubMed...
    49. Zhang W.Y., Yu J.X., Xu Y.J., Wang Z.Q., Liu L.J., Zhang H., Gu J.F., Zhang H., Zhang J.H., Yang J.C. (2021): Alternate wetting and drying irrigation combined with the proportion of polymer-coated urea and conventional urea rates increases grain yield, water and nitrogen use efficiencies in rice. Field Crops Research, 268: 108165. Go to original source...
    50. Zheng E.N., Yang H., Zhang Z.X. (2018): Influence of different nitrogen forms application on rice photosynthesis: fluorescence with water-saving irrigation in black soil region of Songneng Plain, Northeast China. Paddy and Water Environment, 16: 795-804. Go to original source...
    51. Zhou C.C., Huang Y.C., Jia B.Y., Wang Y., Li R.F., Wang S., Feng Y., Dou F. (2019): Effect of interaction between nitrogen rate and irrigation regime on grain quality of japonica rice in northeast China. Chinese Journal of Rice Science, 33: 357-367.
    52. Zou J.N., Pang Z.Q., Li Z., Guo C.L., Lin H.M., Li Z., Chen H.F., Huang J.W., Chen T., Xu H.L., Qin B., Letuma P., Lin W.W., Lin W.X. (2024): The underlying mechanism of variety-water-nitrogen-stubble damage interactions on yield formation in ratoon rice with low stubble height under mechanized harvesting. Journal of Integrative Agriculture, 23: 806-823. Go to original source...

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