Plant Soil Environ., 2026, 72(1):28-38 | DOI: 10.17221/369/2025-PSE

Effect of fertilisation and utilisation methods of red clover on surface nutrient balanceOriginal Paper

Halyna Panakhyd ORCID...1, Nadiia Kozak ORCID...1, Yurii Olifir ORCID...1, Tetiana Partyka ORCID...1, Oleh Havryshko ORCID...1, Hryhorii Konyk ORCID...1, Oleh Stasiv ORCID...1
1 Institute of Agriculture of the Carpathian Region of the National Academy of Agrarian Sciences of Ukraine, Obroshyne, Ukraine

The research was conducted in a long-term stationary experiment established on light grey forest surface-gleyed soil in 1965. Data presented in this study were collected during 2022–2024 growing seasons within the framework of this long-term experiment. The experiment is registered in the NAAS long-term field experiments registry (certificate No. 29) and the Global Long-Term Agricultural Experiments Network (GLTEN). The study examined the effect of growing red clover in a four-field crop rotation on nutrient balance at different fertiliser and lime doses and ratios. Red clover was used for feed and feed-green manure purposes. The research aimed to substantiate optimal methods of utilising this valuable forage crop and optimise fertilisation systems to ensure sustainable agricultural development. Growing the first cut of red clover for feed purposes and the second as green manure with fertilisation (N105P101K101 + organic fertilisers + liming) ensures a positive surface balance of 402 kg/ha of nitrogen, 150 kg/ha of phosphorus, and 204 kg/ha of potassium. These data are almost twice higher than indicators under minimal fertilisation doses. Despite the reduction in symbiotic nitrogen fixation from 217 kg/ha to 147 kg/ha when growing red clover in crop rotation with intensive fertilisation, it remains an effective phytobiological ameliorant.

Keywords: biological fixation; symbiotic activity; leguminous crops; fertilisation systems; surface nutrient balance

Received: August 22, 2025; Revised: January 7, 2026; Accepted: January 8, 2026; Prepublished online: January 27, 2026; Published: January 29, 2026  Show citation

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Panakhyd H, Kozak N, Olifir Y, Partyka T, Havryshko O, Konyk H, Stasiv O. Effect of fertilisation and utilisation methods of red clover on surface nutrient balance. Plant Soil Environ. 2026;72(1):28-38. doi: 10.17221/369/2025-PSE.
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References

  1. Askegaard M., Eriksen J. (2002): Exchangeable potassium in soil as an indicator of potassium status in an organic crop rotation on loamy sand. Soil Use and Management, 18: 200-205. Go to original source...
  2. Azzaroli Bleken M., Francischinelli Rittl T., Nadeem S. (2025): Low N2O emissions induced by root-derived residues compared to aboveground residues of red clover or grass mixed into soil. Soil and Tillage Research, 245: 106309. Go to original source...
  3. Carlsson G., Huss-Danell K. (2003): Nitrogen fixation in perennial forage legumes in the field. Plant and Soil, 253: 353-372. Go to original source...
  4. Carter M.S., Sorensen P., Petersen S.O. (2014): Effects of green manure storage and incorporation methods on nitrogen release and N2O emissions after soil application. Biology and Fertility of Soils, 50: 1233-1246. Go to original source...
  5. Herben T., Goldberg D.E., Münzbergová Z., Klimešová J., Čuda J. (2017): Long-term time series of legume cycles in a semi-natural montane grassland: evidence for nitrogen-driven grass dynamics? Functional Ecology, 31: 1430-1440. Go to original source...
  6. Høgh-Jensen H., Loges R., Jørgensen F.V., Vinther F.P., Jensen E.S. (2004): An empirical model for quantification of symbiotic nitrogen fixation in grass-clover mixtures. Agricultural Systems, 82: 181-194. Go to original source...
  7. Huyghe C., De Vliegher A., Golinkski P. (2014): European grasslands overview: temperate region. Grassland Science in Europe, 19: 29-40.
  8. IUSS Working Group WRB (2022): World Reference Base for Soil Resources. International soil classification system for naming soils and creating legends for soil maps. 4th Edition. International Union of Soil Sciences (IUSS), Vienna, Austria. Available at: https://wrb.isric.org/files/WRB_fourth_edition_2022-12-18.pdf
  9. Karbivska U. (2019): Symbiotic nitrogen fixation of legume-grass mixtures. Visnyk of Lviv National Agrarian University. Agronomy, 23: 72-76. Go to original source...
  10. Kobyrenko Y.O., Kotyash U.O., Panahyd H.Y., Pukalo D.L. (2015): Root mass accumulation of restored grassland depending on sown grass species and fertilization. Mountain and Foothill Agriculture and Animal Husbandry, 58: 126-133.
  11. Lynge M., Lakkenborg Kristensen H., Grevsen K., Nygaard Sorensen J. (2022): Strategies for high nitrogen production and fertilizer value of plant-based fertilizers. Journal of Plant Nutrition and Soil Science, 186: 105-115. Go to original source...
  12. Marshall A.H., Collins R.P., Vale J., Lowe M. (2017): Improved persistence of red clover (Trifolium pratense L.) increases the protein supplied by red clover/grass swards grown over four harvest years. European Journal of Agronomy, 89: 38-45. Go to original source...
  13. McAuliffe C., Chamblee D.S., Uribe-Arango H., Woodhouse W.W.J. (1958): Influence of inorganic nitrogen on nitrogen fixation by legumes as revealed by 15N. Agronomy Journal, 50: 334-337. Go to original source...
  14. McKenna P., Cannon N., Conway J., Dooley J. (2018): The use of red clover (Trifolium pratense) in soil fertility-building: a review. Field Crops Research, 221: 38-49. Go to original source...
  15. Moyo H., Davies W.P., Cannon N., Conway J. (2015): Influences of one-year red clover ley management on subsequent cereal crops. Biological Agriculture and Horticulture, 31: 193-204. Go to original source...
  16. Nyfeler D., Huguenin-Elie O., Suter M., Frossard E., Connolly J., Lüscher A. (2011): Grass-legume mixtures can yield more nitrogen than legume pure stands due to mutual stimulation of nitrogen uptake from symbiotic and non-symbiotic sources. Agriculture, Ecosystems and Environment, 140: 155-163. Go to original source...
  17. Nyfeler D., Huguenin-Elie O., Suter M., Frossard E., Connolly J., Lüscher A. (2017): Strong mixture effects among four species in fertilized agricultural grassland led to persistent and consistent transgressive overyielding. Journal of Applied Ecology, 54: 683-691. Go to original source...
  18. Oenema O., Kros H., de Vries W. (2003): Approaches and uncertainties in nutrient budgets: implications for nutrient management and environmental policies. European Journal of Agronomy, 20: 3-16. Go to original source...
  19. Olifir Y., Habryel A., Partyka T., Havryshko O., Kozak N., Lykhochvor V. (2023): The content of mobile aluminium compounds depending on the long-term use of various fertilizing and liming systems of Albic Pantostagnic Luvisol. Agronomy Research, 21: 869-882.
  20. Png G.K., Turner B.L., Albornoz F.E., Hayes P.E., Lambers H., Laliberté E. (2017): Greater root phosphatase activity in nitrogen-fixing rhizobial but not actinorhizal plants with declining phosphorus availability. Journal of Ecology, 105: 1246-1255. Go to original source...
  21. Poliovyi V.M., Yashchenko L.A. (2022): Potassium balance at different fertilization levels in traditional crop rotation of Western Polissya. Bulletin of NUWEE, 4: 197-208.
  22. Protopish I.G., Kvitko G.P., Getman N.Ya. (2012): Perennial leguminous grasses - an alternative-free predecessor of winter wheat in the conditions of the right-bank Forest-steppe. Feed and Feed Production, 72: 34-39.
  23. Råberg T., Carlsson G., Jensen E.S. (2018): Nitrogen balance in
  24. a stockless organic cropping system with different strategies for internal N cycling via residual biomass. Nutrient Cycling in Agroecosystems, 112: 165-178. Go to original source...
  25. Rasmussen J., Soegaard K., Pirhofer-Walzl K., Eriksen J. (2012): N2-fixation and residual N effect of four legume species and four companion grass species. European Journal of Agronomy, 36: 66-74. Go to original source...
  26. Riesinger P., Herzon I. (2010): Symbiotic nitrogen fixation in organically managed red clover-grass leys under farming conditions. Acta Agriculturae Scandinavica, Section B - Soil and Plant Science, 60: 517-528. Go to original source...
  27. Skersiene A., Slepetiene A., Stukonis V., Norkeviciene E. (2024): Contributions of different perennial grass species and their roots' characteristics to soil organic carbon accumulation. Sustainability, 16: 6037. Go to original source...

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