Ramakrishnan Subhashini author Jebastin Thomas author Raj Sumathy author Ramathilaga Ariyamuthu author Selvaraj Rithik author A. Najat A. Bukhari author Hatamleh Ashraf Atef author Ahamed Anis author text journal article 2024 Carbohydrate digestive enzymes like α-amylase and α-glucosidase can be used to treat and manage diabetes. By inhibiting these enzymes, carbohydrate digestion slowed down, lowering the level of glucose entry into the bloodstream and preventing postprandial hyperglycemia. However, the effectiveness of current antidiabetic agents is limited due to their adverse effects. Therefore, the current study explored natural inhibitors from the methanol extract of rice to combat this issue. Through an integrated approach, four different rice cultivars were analysed and found that red rice methanol extract compounds stigmasterol and 1,2-benzenedicarboxylic acid interacted with α-amylase and α-glucosidase. Additionally, further research on stigmasterol directs the structure-activity relationship studies that aid in managing diabetic conditions. Type2 Diabetes mellitus; hyperglycemic; carbohydrate hydrolysing enzymes; antioxidant activity; molecular interaction 10.17221/150/2024-PSE https://pse.agriculturejournals.cz/artkey/pse-202411-0001.php https://pse.agriculturejournals.cz/artkey/pse-202411-0001.php continuing 70 11 683 694 2024 12141178 periodical academic journal