PT Journal
AU Guan, Q
   Zhou, Y
   Li, S
   Yang, F
   Liu, R
TI Denitrification and Anammox and Feammox in the Yinchuan Yellow River wetland
SO Plant, Soil and Environment
PY 2024
BP 731
EP 738
VL 70
IS 11
DI 10.17221/318/2024-PSE
DE nitrogen cycling; environmental microorganism; functional gene; iron-nitrogen coupling; biogeochemistry
AB Denitrification, anaerobic ammonium oxidation (Anammox), and ferric iron reduction coupled with anaerobic ammonium oxidation (Feammox) are the nitrogen removal pathways in natural ecosystems. In this study, the differences between these three nitrogen removal pathways in a Phragmites australis covered site (LW), artificial grassland covered site (CD), poplar covered site (YD), and topsoil tillage after harvesting reed site (GD) in the Yinchuan Yellow River wetland were investigated using isotope tracing, metagenome, and quantitative polymerase chain reaction (Q-PCR) techniques. No <sup>30</sup>N<sub>2</sub> accumulation was detected in <sup>15</sup>NH<sub>4</sub><sup>+</sup> addition incubations, indicating that Feammox was weak in all sites, which is consistent with a low abundance of the Feammox functional bacteria Acidimiprobiaceae sp. A6. The denitrification rates were 0.36 (LW), 0.5 (CD), 0.76 (YD) and 0.12 (GD) mg N/kg/day. The Anammox rates were 0.18 (LW) and 0.26 (GD) mg N/kg/day; other sites did not detect Anammox rate. Denitrification was the dominant pathway except for the CD site. The YD site had the highest abundance of denitrification genes, which was consistent with the denitrification rate.
ER