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The intensification of Alopecurus myosuroides Huds. (black-grass) is becoming a major problem due to its growing resistance to a broad spectrum of acetolactate synthase (ALS)-inhibiting herbicides. Hence, the present study was conducted to evaluate the resistance level of a black-grass population to iodosulfuron plus mesosulfuron and to identify the underlying resistance mechanism. Dose-response studies revealed that the resistance population is 22 times less sensitive to iodosulfuron plus mesosulfuron than the susceptible population. The probable resistance mechanism identified was the target-site substitution of proline (Pro) by threonine (Thr) at the 197^th position of the ALS enzyme. Furthermore, whole plant response bioassay experiments demonstrated that this population is also resistant to pinoxaden, chlorotoluron, diflufenican plus pendimethalin plus chlorotoluron, fenoxaprop and flufenacet plus diflufenican. In summary, the current findings recommend using alternative herbicides in integrated weed management to interrupt the possible evolution of herbicide resistance in these species.

To study the difference among cytoplasm at the different nitrogen conditions, a research experiment was conducted using five different cytoplasmic male sterile (CMS) hybrid rice with nitrogen levels at N0, N1, N2, and N3; the nitrogen application rates were 0, 90, 180, and 270 kg/ha, from 2018 to 2019. Results showed that among tested cultivars of CMS hybrid rice, JW (J803A × Chenghui727) showed the highest yield in both years for the low nitrogen and high nitrogen treatments. The dry matter accumulation and translation of JW type in nutritive organs were higher than that of others during the low nitrogen level (N1). We concluded that the nutrient translocation within plants organs and dry biomass accumulation were highly dependent on CMS type and nitrogen application. This research indicates that selecting a rice cultivar with greater efficiency of nitrogen is favourable for raising the number of grains per panicle, grain yield, and nitrogen use efficiency. JW cytoplasm displayed great efficiency in low nitrogen, which is a potential cytoplasmic resource.

With the need to obtain new methods to protect seed material from Ditylenchus dipsaci (Kühn, 1857) nematodes, a study was conducted to evaluate the nematicidal effects of several plants' essential oils on the mortality of D. dipsaci. Tests were performed under in vitro conditions; a concentration of 2 000 ppm was tested, nematodes were added into diluted oils, and numbers of living/dead nematodes were scored after 4 and 24 h. The results show a significant effect of several plant essential oils on D. dipsaci mortality, with the highest efficacy found for oil from Cinnamomum cassia ((L.) J. Presl), with 100% mortality observed even after 4 h.

Exogenous carbon (C) not only regulates plant growth but also provides energy for microbes and improves the soil environment. We hypothesised that exogenous C could improve plant growth by affecting the soil environment. Therefore, pot experiments were conducted and peanut cvs. Huayu 22(H) and NN-1(B) were used under three different treatments (the control, single nitrogen (N), and N combined with glucose (CN)). The results showed that the abundance and diversity of N-fixing bacteria are obviously influenced by the C and N, and exogenous C can promote the restoration of microbial diversity. The relative abundances of Burkholderiales were increased under HCN and BCN to 9.8% and 9.5%, respectively, compared to the control (3.9%, 2.5%). The abundance of N fixation bacteria increased mainly due to the soil nutrient change. In comparison with the single N treatment, the addition of the C significantly decreased the soil NH4+-N and NO3--N contents by 31.0% and 13.3%, respectively. And the activities of soil urease and nitrogenase were significantly increased. Compared to the control, single N significantly limited the root development, while the addition of C played a promoting role in root growth. Plant N accumulation increased compared with the control, but there was no significant difference between N treatment and CN treatment. These results indicated that exogenous C promoted soil microorganism activity and strengthened plant growth by changing the soil environment.

Boron (B) is a plant nutrient and a limited mineral resource. Therefore, secondary B sources such as end-of-life cellulose fiber insulation (CFI) should be preferred for B fertiliser production over primary borates. In addition, crop B fertilisation is challenging because B is only weakly adsorbed in soils and prone to leaching in particular if the soil pH is below 7. The objectives of this study were to assess the effect of pyrolysed CFI (B-Biochar) on crop B uptake in the field and on B leaching in a lysimeter study. B-Biochar was pyrolysed at 600 °C and tested (1) in a field experiment with maize (Zea mays L.) and sunflower (Helianthus annuus L.), and (2) in a lab microlysimeter experiment to study B leaching under simulated rainfall. In the field experiment, B concentration in plant tissue increased by up to 100% with B-Biochar compared to the control (from 29.6 to 61.6 mg B/kg in young sunflower leaves) and was only slightly lower (-10% to -20%) than with water-soluble Na-tetraborate (Borax). This lower uptake was attributed to the slow-release properties of the B-Biochar. In the lysimeter experiment, 41% and 55% of added B through B-Biochar was leached below 16 cm depth when fertilised with 1 and 2 kg B/ha, respectively, but B concentration of the leachate remained below the 1 mg B/L threshold value for drinking water in the European Union. In conclusion, CFI has a strong potential as a secondary B source for fertiliser production, and pyrolysis appears to be a suitable process for that purpose. During the processing of CFI to fertiliser, more focus should be given to slow B release in the future in order to reduce losses by leaching.

The paper shows a large-scale shift in agroclimatic zones in the territory of the Czech Republic (CR) between 1961 and 2019. The method used for agroclimatic zoning took advantage of high-resolution (0.5 km × 0.5 km) daily climate data collected from 268 climatological and 787 rain-gauge stations. The climate information was combined with soil and terrain data at the same resolution. The set of seven agroclimatic indicators allowed us to estimate rates of changes in agroclimatic conditions over the 1961-2019 period, including changes in the air temperature regime, global radiation, drought, frost risks and snow cover occurrence. These indicators are relevant for all main crops and agroclimatic zoning and account for local soil and slope conditions. The study clearly highlights major shifts in the type and extent of agroclimatic zones between 1961-2000 and 2000-2019, which led to the occurrence of entirely new combinations of agroclimatic indicators.

Slow-release nitrogen fertiliser can potentially increase crop production and improve fertiliser nitrogen use efficiency. However, it is unclear that are suitable for different regions and crops in the northeast of China. Therefore, according to different soil and climate characteristics, we investigated the synchronised relationships between nitrogen slow release fertiliser and nitrogen maize requirements. Experiments were conducted at Shenyang Agricultural University, Liaoning province, Northeast China, from 2016 to 2017. Stabilised fertiliser treatments increased grain yield, nitrogen use efficiency and nitrogen accumulation at each maize growth stage. Grain yield increased by 2.32% and 11.33% (2016), and 1.55% and 7.87% (2017), respectively, when compared with the urea CK1 (233 kg N/ha) and CK2 (210 kg N/ha) treatments. Additionally, during the growth period of the stabilised fertiliser treatment, the stability of the synchronisation relationship between nitrogen absorption and absorption of spring maize was significantly higher than other treatments, and the effect was the best. Therefore, we conclude that the stabilised fertiliser is the most suitable option for promotion and application in spring maize in Northeast China.

A continuous long-term field experiment (2008-2018) was conducted in Xinjiang, north-western China, to assess the impact of farmyard manure (FYM) and inorganic fertilisers on the sustainable biomass yield of sweet sorghum cultivar (Xingaoliang No. 3) and soil chemical properties. Seven treatments, associated with nitrogen (N), phosphorus (P), potassium (K), FYM, and their different combination, were compared with the control plot (CK). As a result, the treatments NP, PK, NK, NPK and NPKM significantly increased the average biomass yields by 30-48% over CK. The 12 t/ha FYM per year with NPK (NPKM) increased both the yield and total soluble solids (T_SS) by 48% and 7.9%, respectively, while the 18 t/ha/year application rate of FYM had an adverse effect on yield. Stem T_SS, soil available N and K for all treatments decreased while soil organic carbon, soil total salt and the available P for FYM applied treatments increased over the years. The soil pH stabilised at 7.8-8.2 at the end. In conclusion, the 12 t/ha/year of FYM is the most efficient rate for a single application or incorporation with inorganic fertilisers. A more reasonable application rate of N and K fertiliser to increase the yield and irrigation rate to reduce soil salt needs for further investigation.

Although various studies of codon usage bias have been reported in a broad spectrum of organisms, no studies to date have examined codon usage bias for herbicide target genes. In this study, we analysed codon usage patterns for the acetolactate synthase (ALS) gene in eight monocot weeds and one model monocot. The base composition at the third codon position follows C3 > G3 > T3 > A3. The values of the effective number of codons (ENC or Nc) indicate low bias, and ENC or Nc vs. GC3 plot suggests that this low bias is due to mutational pressure. Low codon adaptation index and codon bias index values further supported the phenomenon of low bias. Additionally, the optimal codons, along with over- and under-represented codons, were identified. Gene design using optimal codons rather than overall abundant codons produce improved protein expression results. Our results can be used for further studies, including eliciting the mechanisms of herbicide resistance (occurring due to elevation of gene expression levels) and the development of new compounds, their efficiency and risk assessment for herbicide resistance evolution.

Finding an appropriate method with the highest rate of polycyclic aromatic hydrocarbon (PAH) removal from naturally polluted soils is an important research issue. A pot factorial experiment (using contaminated soil samples from the Isfahan Refinery, Iran) was conducted in a 90-day period to compare the following bioremediation strategies: (1) natural attenuation (NA): the inherent ability of soil for bioremediation; (2) bioaugmentation (BA): inoculating soil with PAH degrading microbes Marinobacter hydrocarbonoclasticus; (3) biostimulation (BS): using N, P and K nutrients for the stimulation of bioremediating soil bacteria to achieve the C:N:P ratio of 100:10:1, and(4) bioaugmentation + biostimulation (BS + BA). Treatments NA (22.8%) and BA + BS (63.9%) resulted in the least and the highest rate of PAH removal from the soil. The 2-4 ring compounds had a significantly (P ≤ 0.05) higher rate of degradation than the 5-6 ring compounds. The highest rates were resulted by fluorene (76.41%) and acenaphthylene (72.28%) using the BA + BS treatment. However, the lowest degradation rates were resulted by indeno (1,2,3-cd) pyrene (10.05%), benzo [b] fluoranthene (10.17%), benzo (g, h, i) perylene (12.53%), and benzo [k] fluoranthene (13.67%), using NA treatment. The BA + BS treatments are the most effective method for the bioremediation of PAH polluted soils.

The honey bee is one of the insects that is significantly endangered by the application of pesticides in the cultivation of crops. Not only is acute toxicity dangerous, but the importance of chronic poisoning by low doses of pesticides in hives is growing. The behavior of bees can be affected not only by insecticide residues but also by herbicide and fungicide residues. In 2016-2018, samples of bee bread were analysed for pesticide content at 25 different localities from intensive agricultural production areas of the Czech Republic. Substances were extracted by QuEChERS and determined by liquid chromatography, together with mass spectrometric detection. We detected up to 18 pesticides in one sample. In total, during 2016-2018, we identified 53 active substances. Fifteen substances (31%) were herbicidal, 23 substances (47%) of fungicidal nature and 6 substances (12%) of insecticidal nature. The coefficient of variation showed large differences in the frequency of revealed pesticides between years. For substances sprayed outside period attractive for pollinators (mainly herbicides and some fungicides), the usual methodology cannot reliably determine the degree of contamination, and thus the actual contamination with these substances may be even higher than demonstrated in this study.

Agronomic bio-fortification is one of the main approaches for mitigation of micronutrient shortage in human populations and endorses sustainable production of food and feed. Studies related to agronomic bio-fortification of crops are mainly focused on single or rarely two micronutrients application, and no attempt has made to study the combined effect of zinc (Zn), iron (Fe) and selenium (Se) on forage sorghum. Therefore, this research was accomplished to evaluate the effect of Zn, Fe and Se bio-fortification on diverse sorghum accessions. The field experiments were conducted in a randomised complete block design with a split-plot arrangement. The treatments comprised of Zn (10 mg/L as ZnSO₄∙5H₂O), Fe (7 mg/L as FeSO₄∙7H₂O), Se (3 mg/L as SeSO₄) and CK (control) were applied to five sorghum accessions: G₁ (Y-16), G₂ (YSH-166), G₃ (YSH-134), G₄ (YSS-98) and G₅ (YSH-132). According to our results, the sorghum accession G₅ showed superiority over all other accessions and produced maximum values of all growth and quality traits except grains number per panicle and 1 000-grain weight. All applied micronutrients (Zn, Fe and Se) enhanced the growth, quality and uptake of nutrients in sorghum accessions. However, Se recorded the highest plant height, stem diameter, 1 000-grain weight and Zn produced the maximum protein, oil and starch contents. Conclusively, it can be concluded that G₅ with Se must be used to achieve the optimum values of agronomic traits, while G₅ with Zn found more effective to improve the quality traits of sorghum.

Insect pollination is one of the most important ecosystem services. Pollinator communities are rarely studied across years and crops in the same location. The aim of this study was to investigate the pollinator community structure on five different fruit crops, as well as the activity of different pollinator groups during the day and their temperature preferences. Pollinator activity was observed across two years on apple trees and blueberry, strawberry, blackcurrant and raspberry bushes. Pollinator community structure varied by plant and year. Honeybees were the most numerous pollinators on all plants except blueberry bushes (39-95% of visits). Bumblebee numbers were high on blackcurrant (up to 28%) and blueberry bushes (up to 61%). Solitary bees visited all plants except blueberries. Honeybees, solitary bees, and hoverflies were most active in the middle of the day, while bumblebees became active earlier in the morning and remained active later in the evening. Pollinators also differed greatly in their temperature preferences. This knowledge gained is necessary for less harmful pesticide application management and the development of more sustainable agriculture to maintain pollinator diversity and thus reliable pollination in extreme weather conditions.

A new food and feed self-sufficiency model for the Czech Republic (RESTEP) was applied for the evaluation of possible adverse climate impacts uniformly reducing crop production by 5, 10, 20, and 30%. The situation was simulated for the whole country and four different agriculturally important regions. Biomass production modeling confirmed that for the whole country, the food self-sufficiency is secured up to 20% of yield decline for most crops, but even 5% yield decline of silage maize would lead to its shortage in animal feeding. On the other hand, regional results vary significantly. Regions Jižní Morava and Střední Čechy shown oversupply of feedstuff allowing them to cover the demands of cattle and pigs up to 20% or 30% decline of yield, respectively. The opposite model represents the Vysočina (VY) region which is not able to cover the demands from own sources even at the baseline scenario. The acreage extension of maize is not possible due to erosion risk restrictions at 25% of arable land at VY. The possible solution consists of extension acreage of alfalfa and clover or finding other plants sufficient for feeding as well for biogas facilities in regions rich in biomass energy consumers.

Secondary fertilisers are becoming an important alternative to conventional mined fertilisers. For the first time, the struvite "relative" hazenite (KNaMg₂(PO₄)₂∙14 H₂O) has been artificially synthesised. A pot trial assessed whether hazenite-fertilised ryegrass had comparable potassium (K), magnesium (Mg), or phosphorus (P) uptake and shoot yields with treatments receiving conventional K (muriate of potash), Mg (kieserite) or P (triple superphosphate, TSP) fertilisers. Ryegrass shoot biomass production and K/Mg uptake in replicates receiving hazenite were as good as or superior to those amended with conventional fertilisers. Phosphorus uptake of plants whose P source was TSP was significantly higher than that of the hazenite-amended replicates without significantly higher shoot biomass, indicating luxury P uptake. Hazenite's constituent sodium (Na) makes it a potentially useful soil amendment for forage grasses or natrophilic crops such as sugar beet. Its component Mg would also be desirable in forage grasses to pre-empt ruminant hypomagnesemia or in crops with a relatively high Mg demand, for example, maize. Furthermore, hazenite represents a good alternative to KCl for chlorophobic crops such as potatoes. However, given its unusual ratio of Mg, P, and K, the ideal application of hazenite would probably be in combination with other fertilisers.

A reliable tuber yield prognosis requires a complex statistical analysis of potato nutritional status in the fully developed 4^th leaf at the onset of tuberisation. This hypothesis was validated in the series of field experiments conducted in 2006-2008 in Poland. The experimental design was composed of two nitrogen (N) rates (60, 120 kg/ha), two N fertilisers (Urea and Agrotain), two rates of sulfur (0, 50 kg/ha). The marketable tuber yield of cv. Zeus ranged from 31.3 to 59.3 t/ha in 2008 and 2006, respectively. Despite annual variability, the potato presented a good nutritional status. In 2008, the contents of N, Mg, Cu and Zn were about 33% lower as compared to 2006. The stepwise and path analyses indicated N, Mg and Cu as the key yield-limiting nutrients. The diagnosis and recommendation integrated system (DRIS) showed that a slight imbalance of N and Mg did not disturb tuber yield, provided a positive balance of K was maintained. The Mg index, as a result of the DRIS procedure, emerged as the best single predictor of potato yield.

Agronomic zinc (Zn) biofortification of crops could help to alleviate dietary Zn deficiency, which is likely to affect more than one billion people worldwide. To evaluate the efficiency of agronomic Zn biofortification of broccoli, four application treatments were tested: no Zn application (control); soil application of 5 mg/kg ZnSO₄.7 H₂O (soil); two sprays (15 mL/pot each) of 0.25% (w/v) ZnSO₄.7 H₂O (foliar); and soil + foliar combination. Soil Zn application increased Zn-DTPA (diethylenetriamine pentaacetic acid) concentration by 3.7-times but did not affect plant growth or plant Zn concentration. Foliar Zn application increased stem + leaves and floret Zn concentration by 78 and 23 mg Zn/kg, respectively, with good bioavailability based on phytic acid concentration. Boiling decreased mineral concentration by 19%, but increased bioavailability by decreasing the phytic acid concentration. The entire broccoli could constitute a good nutritional source for animals and humans. An intake of 100 g boiled florets treated with the foliar treatment will cover about 36% of recommended dietary intake (RDI) of Zn, together with 30% of Ca, 94% of K, 32% of Mg, 6% of Na, 55% of P, 60% of S, 10% of Cu, 22% of Fe, 43% of Mn, and 35% of Se RDIs.

In the years 2011-2014, winter wheat grain yield, qualitative and economic parameters were evaluated according to different fertiliser treatments: (1) control: unfertilised treatment; (2) farmyard manure (FYM) and (3) FYM + NPK (farmyard manure applied together with mineral NPK). The highest yields (8.10 t/ha) were recorded in the FYM + NPK treatment, while significantly lower yields (6.20 t/ha and 5.73 t/ha) were recorded in FYM and control treatments, respectively. Similarly, statistically significantly higher values of the quality parameters were found in the FYM + NPK treatment (13.55% of crude protein content and 43.56 mL of Zeleny's sedimentation test), compared to control (10% and 22.44 mL, respectively). The modelling expert system (AGROTEKIS-Crop Technology and Economy) was used for the evaluation of economy. This software is based on technological methods of cultivation and norms of material input costs and costs of individual mechanised works. The economic benefits and profitability were evaluated for three different levels of grain market price. The highest gross profit per ha was recorded in the FYM + NPK treatment. According to the gross profit, the control treatment provided better results than the FYM treatment.

One of the most serious threats facing agricultural productivity in the world is unfavourable soil conditions. Several studies have shown that almost half of the world's land-mass is affected by either natural or human-induced pollution. This, therefore, poses a threat to agricultural improvement needed to tackle the problem of a continuous increase in the world population. The emergence of soil extremophiles with plant growth-promoting trait has proven to be a reliable means to quell the threat posed by some factors limiting soil potency. Adopting these organisms as bio-inoculants will easily proffer a solution to both biotic and abiotic soil stress. As such, the natural bio-fertilisers will help to improve the quality of the soil by making it healthy enough to sustain sufficient plant growth. This review gives an overview of the multifarious importance of extremophiles on plants grown under harsh soil conditions, with the multifaceted application of omics as a means to unveil these organisms and their benefits for environmentally sustainable agricultural systems and food safety.

The presence of Epichloë endophyte can promote plant growth and increase the accumulation of host plant nutrients. We determined the dry matter (DM) and important nutritional indicators of E+ (infected by endophyte) and E- (not infected by endophyte) Festuca sinensis under the three-time repeated cutting. The results indicated that the total nitrogen, total phosphorus, crude protein (CP), crude fat (CF), crude ash (CA) contents, and DM of F. sinensis decreased with the repeated cutting increase and reached the minimum after the third time cut. The total organic carbon content of F. sinensis peaked at the second time cut. In addition, the DM of F. sinensis was significant (P < 0.05) positively correlated with its quality CP, CF, and CA contents, and the appropriate repeated cutting times of F. sinensis was 1-2 times. We concluded that the presence of endophyte and proper cutting frequency can increase the quality and biomass of F. sinensis in Western China.

The aim of this study carried out in 2017-2018 was to determine the effect of white cabbage (Brassica oleracea L.), which is known to have an allelopathic effect on the germination of some weed and cultural crops seeds. In addition to weeds, Amaranthus retroflexus L., Chenopodium album L. and Solanum nigrum L., cultural plants such as Zea mays L., Beta vulgaris L., were used in this study. It has been shown that the seed germination rate was decreased by the increased concentration of white cabbage post-harvest leaves extractions, which were made with different concentrations of aqueous and methanol extracts (30, 40, and 50%). The methanol extract was found to be more effective in germination. According to these results, S. nigrum and Z. mays seeds were less affected than A. retroflexus, C. album and B. vulgaris seeds. In the light of these data, it is observed that extracts obtained from white cabbage can be effective on some weeds; however, it was concluded that in order to reach more definite conclusions, studies on the subject should be increased, and similar studies should be continued under greenhouse or field conditions.

Dung deposited by grazing animals is a key driver affecting sward structure and nutrient cycling in pastures. We tested herbage and soil properties in three types of tall sward-height patches (> 10 cm): (i) patches with dung under intensive grazing; (ii) patches with dung under extensive grazing; and (iii) patches with no dung under extensive grazing. These patches were compared with grazed swards under intensive and extensive grazing. Analyses indicated no significant effect of different types of patches on plant available nutrients. Herbage nutrient concentrations from the different types of patches differed significantly. The highest concentrations of nitrogen (30.65 g/kg), phosphorus (4.51 g/kg) and potassium (22.06 g/kg) in the herbage dry matter were in the tall patches with dung presence under intensive grazing regime because of nutrients from dung utilized for sward regrowth. Regardless of dung presence, similar herbage nutrient concentrations were revealed in non-grazed tall sward-height patches in extensive grazing regime. The presence of dung did not have any effect on the plant available nutrients in any type of patches, therefore we suppose that non-utilized nutrients were probably leached, volatilised or transformed into unavailable forms and thus soil nutrient enrichment was low.

To find out exactly how sowing density and sowing pattern affect soybean grain yield, quality and its components in non-traditional soybean growing regions, such as Germany, two field trials have been conducted at two locations in Bavaria in 2016 and 2017. The experiments were carried out with four sowing densities (30, 50, 70, 90 seeds/m²) and four cultivars from different maturity groups (Viola 000, Lissabon 000, ES Mentor 00, Orion 00) as randomized complete block design with four replicates. Almost all evaluated traits varied significantly depending on year × location. There was no interaction between the main factors (cultivar × sowing density). The results revealed that grain yield and height of the first pod increased with increase of sowing density. The number of pods per plant and branching decreased with increasing sowing density. At higher sowing densities at flowering leaf area index was significantly higher than at lower sowing densities. The cv. ES Mentor (00) with 70 seeds/m² has proved to be a suitable cultivar in terms of yield and quality in southern Germany (> 3.6 t/ha grain yield and 40% protein).

In a two-year pot experiment, the effect of five growing media on the growth, flowering, decorative value of Pelargonium peltatum cv. Maxime as well as on their uptake of the nutrients and heavy metals were studied. The media were prepared from four composts (made from: sewage sludge 70% or 35%, potato pulp 35%, straw 30% or sawdust 30%) and peat in 1:1, V:V ratio. In the 1^st year of research 7-month-old composts and in the 2^nd year 18-month-old composts were used. Plants cultivated in 7-month-old composts showed better growth-related parameters, created more inflorescences and were more decorative than those cultivated in 18-month-old ones. The medium with compost consisting of 70% sewage sludge and 30% straw gave the best results. Composts application increased nutrients and heavy metals content in pelargonium leaves. Heavy metals content was definitely lower than the value considered toxic to plants.

Several sets of soil samples were chosen to demonstrate the applicability of the Mehlich 3 extractant for the determination of sulphur (S) in soils. Archived samples from 139 basal soil monitoring plots (BSMS) sampled in 1995 and 2013, samples from eleven long-term field trials sampled in 1981 and 2017, 1167 soil samples from the areas vulnerable to S losses and 720 samples from the non-vulnerable areas sampled in 2010 were chosen for the experiments. Mehlich 3 clearly showed a statistically highly significant decrease in the soil S content caused by reduction of SO₂ emissions in the long-term field experiments from 33 mg/kg in 1981 to 8 mg/kg in 2017 for the median of the untreated controls. Similar results were obtained for BSMS samples, where an average decrease from 26 mg/kg in 1995 to 17 mg/kg in 2013 was found. Mehlich 3 also showed that more than 52% of samples from the areas vulnerable to S losses were in a very low content category in contrast to only 3% of soils from the other areas. Mehlich 3 clearly proved the capacity to distinguish changes in the content of soil S in all studied cases.

Influence of plant growth regulators chlormequat chloride, chlormequat chloride + ethephon, ethephon, and mepiquat chloride + prohexadione-Ca + pyraclostrobin + ammonium sulphate (BAS67800F + BAS00800D) on decreasing sunflower height was evaluated. It was determined that sunflower height can be reduced by as much as 30 cm. In the case of BAS67800F + ammonium sulphate, there was a slight difference between application at BBCH 31-33 and BBCH 50-51, whereas for ethephon better application time was at BBCH 50-51. For chlormequat chloride, application at BBCH 31-33 was better, but height reduction did not endure until harvest. Flower head diameter shortly before harvest was not affected at any tested regulator. Flowering was delayed primarily at applications at BBCH 50-51.

Soils of forest ecosystems can release or consume methane (CH₄) depending on their specific hydrological regime. Our study reported the consumption of CH₄ by soil in a lowland broadleaf mixed temperate forest in the Czech Republic (Central Europe). The motivation of our study was to determine the importance of CH₄ fluxes in context of carbon dioxide (CO₂) fluxes of a broadleaf mixed forest. CH₄ and CO₂ emissions from the soil were measured during the 2016 vegetation season on a long transect applying the chamber technique. The average daily consumption of atmospheric CH₄ by the forest soil ranged from 0.83 to 1.15 mg CH₄-C/m²/day. This consumption of CH₄ during summer and autumn periods was not significantly affected by soil temperature and soil moisture. However, during spring period the consumption of CH₄ was positively significantly affected by soil temperature and moisture. Estimated amount of carbon (CH₄-C) consumed by the forest soil makes up a very small part of carbon (CO₂-C) participated in the ecosystem carbon cycle.

Mehlich 3 is an extractant used worldwide for extracting bioavailable nutrients in soils; however, its extraction abilities for sulfur (S) are still not well described. The aim of this preliminary study was to compare the results of Mehlich 3 determined soil S fraction (S_M3) with the results of sulfur fractionation, mainly focusing on bioavailable S (S_av - sum of water-extractable (S_w) and adsorbed (S_ads) sulfur). Air dried soil samples from commonly used agricultural soils were chosen for the analyses. The following S fractions were determined: (i) S_w; (ii) S_ads; (iii) S_av; (iv) 1 mol/L HCl extractable (S_HCl); (v) estersulfate (S_es); (vi) organic (S_org) and (vii) total (S_tot). The median value of S_M3 (18.3 mg/kg) was similar to S_av (17.9 mg/kg). From the correlation and regression analysis it is clear that S_M3 results are in close relationship with S_av form. On the other hand, the relationships between S_M3 and organic S (including S_HCl) were very weak. Based on the obtained results it can be concluded that Mehlich 3 method has a good potential to determine bioavailable sulfur in commonly used agricultural soils. However, especially the plant response should be further studied to confirm this theory.

The boron (B) availability and uptake were studied in relation to different phosphorus rates applied into soils in a three-year field experiment (2015-2017). The experiment was carried out at the experimental station at Humpolec (Bohemian-Moravian Highlands, Czech Republic). Three rates of phosphorus (20-40-80 kg P/ha) were applied as triple superphosphate. The crop rotation was spring barley-winter oilseed rape-winter wheat. No systematic fertilization with B was used and the response of natural boron soil content to the different phosphorus supply was studied. The crop yields, B content in plants, B-uptake, and content of B (extracted by Mehlich 3 and NH₄ acetate methods) were determined. Spring barley and winter wheat B uptake was about one order of magnitude lower in comparison with oilseed rape. Significant differences in B content in soils, in crop tissues and B-uptake, were found mainly under higher phosphorus doses (40 and 80 kg P/ha). NH₄ acetate method showed better correlations between P and B contents in soils than Mehlich 3 method from the second experimental year. The P-fertilization may affect negatively the B-uptake by plants, particularly if the highly nutrient demanding crop is grown.

The paper deals with a general solution of semantic description related to various electronic data formats in the domains of agriculture, aquaculture, forestry, food industry, environment, horticulture and rural areas. The solution presented was developed on the basis of metadata formats analysis and then complemented with its own software superstructure. It is based on the VOA³R metadata application profile (AP) that was developed within the framework of the Virtual Open Access Agriculture and Aquaculture Repository project for the sake of describing research papers and scientific publications. However, thanks to its complexity and comprehensiveness, it is also suitable for different kinds of data and while combining it with the AGROVOC thesaurus, which is elaborated by the Food and Agriculture Organization of the United Nations, it can become a robust and universal tool for data characteristics and semantic description. The potential of the proposed solution is illustrated by means of two examples. While the first example brings a metadata description of a photograph depicting anthocyanin pigmentation of barley straw caused by phosphorus deficiency, the second example is related to a research paper description. The developed system of metadata description is broadly applicable in agriculture such as in precision agriculture, in plant production or in ground cover monitoring and evaluation based on sensor or visual data.