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When 2-chloro-6-(trichloromethyl) pyridine (nitrapyrin) is applied alone, it typically does not significantly increase crop yield. Therefore, we combined gamma-aminobutyric acid (GABA) with nitrapyrin to address the limitations of nitrapyrin in enhancing yield. We conducted indoor incubation experiments and pot experiments in Chernozem and Calcic Kastanozem, respectively. The results demonstrated that GABA exerted an influence on the effectiveness of nitrapyrin by altering its degradation rate. In Chernozem, GABA accelerated nitrapyrin degradation, whereas, in Calcic Kastanozem, the results were the opposite. The pot experiment results showed that the combination of nitrapyrin and GABA increased rice total biomass by 5%, grain yield by 18 ± 2%, and plant nitrogen (N) uptake by 9 ± 1% compared to nitrapyrin applied alone. The increase in yield was attributed to the combined effect of nitrapyrin and GABA, which elevated root biomass and leaf area. In contrast, the effect of GABA on yield through altering the degradation rate of nitrapyrin was weaker. Therefore, the combination of nitrapyrin and GABA combined with urea increases rice yields in Chernozem and Calcic Kastanozem. The aim of this endeavour was to foster the development of a novel fertiliser product that offers both favourable agronomic outcomes and environmental benefits.

Using biostimulants to enhance plant growth and increase yield and secondary metabolites in medicinal and aromatic plants is an important strategy to achieve sustainable agriculture. The influence of two strains of nitrogen-fixing rhizobacteria (NFB) of Azotobacter chroococcum (NFB1) and Azospirillum lipoferum (NFB2), three levels of seaweed extract (SWE; 0 (SWE1), 250 (SWE1), and 500 mg/L (SWE2)) and their interactions have been investigated on Trachyspermum ammi L. (ajwain) growth, fruit yield, and essential oil constituents for two winter seasons. Growth traits (plant height, number of branches, and fresh and dry weights) and fruit traits (umbel number, 1 000-fruit weight, and fruit yield) were improved following NFB and/or SWE applications. Leaf pigments, total phenols, carbohydrates, free amino acids, and nutrient content were also enhanced. Ajwain plants that received NFB2 soil inoculation and foliarly sprayed with SWE1 observed the highest growth and yield values. Applying this treatment resulted in 27.6% and 32.7% higher fruit yield per plant for the first and second seasons, respectively, compared to the control. The results of GC-MS revealed that γ-terpinene, p-cymene, and thymol are the major components in ajwain essential oil. All applications used changed the percentages of the main components detected in ajwain essential oil. For instance, increasing SWE level caused a reduction in γ-terpinene with an increase in thymol content. The highest conservation rate from γ-terpinene to thymol was detected in NFB2 × SWE1-treated plants, with the highest thymol content and least γ-terpinene. Azospirillum lipoferum soil inoculation with SWE1 foliar application is recommended to enhance ajwain production, in terms of fruit yield and oil quality.

The effects of biogas residue as a substrate on ginseng growth and its feasibility for ginseng cultivation are unclear. The present study used biogas residue at different concentrations and maturity levels to cultivate ginseng. The biological characteristics of ginseng, soil physiochemical indices, and ginseng and soil microbial communities were investigated. The results showed that with increasing ginseng content and maturity, the total fresh weight, total length and saponin content significantly increased. The enzyme activities of soil, NO₃^–-N, and available phosphorus also increased. The microbiome analysis revealed that with the addition of biogas residue, microorganisms related to plant growth promotion, such as Chloroflexi, Gemmatimonadota and Mortierellomycota, were more common in the plant or rhizosphere soil. The results based on the co-occurrence network showed that the structure of the bacterial community was more stable than that of the fungal community with increasing biogas residue content. Our results indicated that biogas residue could be used as a ginseng cultivation substrate and promote growth.

Copper contamination stemming from copper-based pesticides poses a grave concern in vineyards and orchards, causing toxicity to soil organisms. Here, we present a comprehensive review of global data encompassing copper levels in these soils, coupled with variables such as the age of agricultural establishments, climate, soil organic matter content, soil pH, and farming practices (organic vs. conventional). The results suggest that there are three pivotal determinants driving copper content in vineyard and orchard soils: climate, the age of agricultural establishments, and soil organic matter content. It was impossible to estimate soil pH’s effect on soil copper content because of its dependence on precipitation. Copper content in vineyard and orchard soils worldwide follows a direct correlation with precipitation while inversely correlating with aridity (i.e. potential evapotranspiration divided by precipitation). Furthermore, a clear linkage emerges between farm age and increased copper content in soils globally. Intriguingly, the increased soil organic matter content has shown inverse impacts on soil copper levels. These effects of soil properties on soil copper contents were discussed in terms of copper losses from soil via surface runoff. However, no discernible disparities in soil copper content between organic and conventional farming systems were found. This worldwide survey not only underscores the established influence of climate on European vineyards but also sheds novel light on the historical legacy of copper contamination in these landscapes.

Standard roses are a widely used ornamental plant in urban landscapes, valued for their attractive flowers and adaptability to various environmental conditions. This study investigated how different substrate types affect the growth and development of standard roses and their potential to improve the ecology of urban landscapes. Nine substrate conditions (rotted corn stover, decomposed shiitake mushroom residue, perlite, and combinations) were compared with field soil as a control treatment. The physical and chemical characteristics of each substrate were analysed, and the growth and development of standard rose plants were observed over six months. The results indicated that the substrate T4 (70% rotted corn stover, 15% decomposed shiitake mushroom residue, 15% perlite) achieved the highest evaluation index, leading to superior plant growth compared to other substrates. This combination provided optimal water retention, aeration, and nutrient supply, making it the most effective substrate for cultivating standard roses. Additionally, the use of these substrates can improve soil quality and reduce environmental pollution, offering a sustainable option for urban landscape management.

Bromus sterilis L. (barren brome) is one of the most economically important noxious grass weeds in the winter cereal fields of Europe. Its ecological behaviour in this agro-climatic region should be assessed for effective weed control strategies. The present study was conducted to assess the dormancy and germination response of the B. sterilis population from the Czech Republic under thermal, light, and stress conditions. The dormancy loss experiment revealed that seeds exposed to the light regime showed a remarkably lower percentage of germination, and under alternating temperatures of 10/20 °C in dark conditions, rapid loss of primary dormancy was observed. This population was found to germinate across a wide temperature range of 5–35 °C, with the highest germination rate at 25 °C (T50 = 1.14 days in dark, 1.21 days in light) and the germination time increased with decreasing temperatures below 25 °C. Further, due to fitness advantage, herbicide-resistant (R) biotypes were found to be more stress-tolerant than susceptible (S) biotypes under salinity and drought conditions. In the highest stress conditions, the germination of S biotypes was negligible, while R biotypes can germinate under high stress, but germination decreased below 25 °C. The current findings may add value to effective weed control strategies using prediction models based on seed dormancy and germination values under different hydrothermal conditions.

A pot experiment was carried out to determine the effect of Serendipita indica on the salt response of Lolium multiflorum Lam. Although the salinity decreased the root colonisation of S. indica by 28.34%, successful colonisation of S. indica increased the seed germination rate, fresh weight, leaf relative water content and chlorophyll content by 28.09, 59.01, 38.78 and 28.80%, respectively, compared with uncolonised seedlings. Under salinity, leaf malondialdehyde content, leaf relative electrical conductivity, as well as Na+ content and Na⁺/K⁺ ratio in leaves and roots of S. indica-colonised seedlings were decreased by 33.99, 33.31, 63.40% and 47.42, 85.66 and 55.88%, respectively, compared with uncolonised seedlings. Meanwhile, compared with uncolonised seedlings under salinity, the contents of proline in leaves, N, P and K⁺ in leaves and roots of the S. indica-colonised seedlings were increased by 47.47, 45.69 and 30.05%, and 41.77, 19.51, 19.18 and 155.00%, respectively. These results indicate that S. indica colonisation confers salt tolerance in L. multiflorum seedlings by enhancing osmotic adjustment via actively accumulating proline and K⁺, increasing the uptake of nutrients such as N and P, and improving Na⁺/K⁺ homoeostasis. The study would provide a new idea for the combined application of salt-tolerant plants and symbiotic microorganisms in the ecological restoration of saline-alkali lands.

Our objectives were to characterise arable weeds of the Czech and Slovak Republic in cereals, especially considering important groups of species: thermophilic, weedy grasses, and rare and endangered species. The three-year phytocoenological survey was conducted from 2006 to 2008 in five climatic regions and eight different soil types. 379 relevés were recorded, and 281 weed species were found. The effects of both the climatic region and soil type were found to be statistically significant. Species richness increased with altitude. Several species were distinctly associated with soil type, from fertile chernozems to less fertile cambisols, while fluvisols were strongly associated with e.g. Calystegia sepium (L.) R. Br. The incidence of species outside their expected regions generally fit two categories: the most pervasive weeds found almost in all climate regions, such as Echinochloa crus-galli (L.) P. Beauv., or rare weeds in higher altitudes than expected, such as Lolium temulentum L. Rare and endangered species were more likely to be found in a colder climate and poorer soils, and this is linked to less intensive management; however, not all endangered arable species can tolerate such conditions. Even though this data is not recently collected, this is still valuable information on the distribution of weedy species, especially concerning the current interest in maintaining biodiversity.

This work aimed to evaluate the influence of soil characteristics and the applied amount of nicosulfuron on the degradation rate in soil. Soil samples were collected at three localities in Bosnia and Herzegovina – Manjača, Kosjerovo and Tunjice. The experiment was carried out under controlled laboratory conditions. Plant protection product based on nicosulfuron (40 g a.s./L, OD) was applied in concentrations of 0.075, 0.15, and 0.30 mg a.s./kg of soil. Nicosulfuron residues were analysed by the modified QuEChERS method, followed by LC-MS/MS. Soils are classified as silty loams, with variations in mechanical composition and chemical properties. In slightly alkaline soil, the half-life (DT50) of nicosulfuron has increased (43.31 days) compared with DT50 (9.43–16.13 days) in acidic soils. The results indicate that soil characteristics and applied concentration significantly influence nicosulfuron persistence. Hence, it can be considered that nicosulfuron, applied to silty loam soils of Bosnia and Herzegovina, poses a low risk to subsequent crops and the environment.

Grasslands are the predominant land use type in China, which is currently encountering significant desertification issues. Consequently, restoring grassland vegetation has important implications for terrestrial carbon (C) levels and, consequently, the global C balance. This study focused on Salix cupularis, the primary plant used for desert control on the eastern edge of the Qinghai-Tibet Plateau. We analysed the rhizosphere and non-rhizosphere soil up to the depth of 60 cm after Salix cupularis growth for 0–24 years, examining soil total organic carbon (TOC) and its labile fractions. Following restoration, there was a gradual increase in TOC and its labile fractions, with the most significant changes observed in the rhizosphere soil at a depth of 0–20 cm. After 24 years of restoration, the TOC content in both rhizosphere and non-rhizosphere soil had increased by 141.74% and 39.44%, respectively. Labile organic C in the rhizosphere soil increased more rapidly and pronouncedly compared with the TOC. Specifically, dissolved organic C and easily oxidised organic C in the rhizosphere soil saw substantial increases of 211.03% and 217.65%, respectively. Meanwhile, compared with the 4 years of restoration, soil C pool management index of the 8–24 years soils increased, ranging from 15.70% to 132.21%. Therefore, long-term vegetation restoration on the eastern margin of the Qinghai-Tibet Plateau can significantly enhance TOC and its labile fractions, as well as improve soil C sink capacity and quality.

The presented research deals with the effect of plot size changes on the crop yield. Three plots were chosen in a company engaged in conventional agriculture, on which yields were monitored from 2019 using yield maps. In 2020, the plots (initial size > 30 ha) were divided into different parts sized < 30 ha. In 2021, these newly arisen parts of the plots were harvested. Changes in the yield of grown crops were analysed using yield maps acquired by the harvesting machines. Relative yields (%) and absolute yields (t/ha) were determined on all experimental land parts arising from the initial plots’ division. The values were then compared with yields recorded before the division of individual plots using zonal statistics. Measured relative yield values clearly show (P < 0.05) that the division of plots resulted in the increased heterogeneity of crop yields. On the initial plots as well as on the newly arisen plots, the relative yield was divided into the following categories: < 70, 70–85, 85–95, 95–105, 105–115, 115–130 and > 130%, with the value of 100% representing average yield. The analysis of measured yield data showed that the division of plots into smaller parts resulted in an uneven yield distribution because if a divided plot was heterogeneous in terms of yield levels, a cumulation of "higher yield levels (> 100%)" could have occurred in one specific newly arisen plot at the expense of another one. Moreover, new marginal parts of lands came into being during the division of larger soil complexes, and hence zones with potentially reduced yields.

The effects of the foliar application of plant growth regulators (PGRs), salicylic acid (SA) and ascorbic acid (AA) were studied on yield and some qualitative traits of corn silage under drought stress in a field experiment conducted in the Agricultural and Natural Resources Research Center of Khoy in two consecutive years. The experiment was performed in four replications as a split plot in a randomised complete blocks design. Irrigation treatment was in two levels, ir75 and ir150, and the foliar applications of SA and AA at seven levels (100, 200, and 300 ppm, as well as a control treatment). The amount of water consumed in ir75 and ir150 during 10 and 7 times irrigation was 6 000 and 4 200 m3/ha, respectively. Malondialdehyde (MDA) content was increased over the plant growth period in both ir75 and ir150, but with the difference that its range was 3.72 to 12.9 nmol/g FW (fresh weight) under ir75 and 12.5–109.5 nmol/g FW under water shortage conditions. The results show that ir150 decreased plant height, forage yield, ear weight, and nitrogen uptake versus ir75. In plants treated with SA and AA, nitrogen uptake and chlorophyll content increases (45–33%) were observed compared to the control plants under ir75. In most traits, there was no significant difference between AA and SA levels, but plants treated with SA100 showed higher protein yield, dry forage yield, and ear yield.

New cropping practices such as single-grain seeding, lower seed densities with stronger cultivars, wide inter-row spacings and camera-guided side-shift control for weed hoeing facilitate mechanical weed control in oilseed rape. In a two-year field study in Southwestern Germany, camera-guided inter-row hoeing was compared to standard herbicide treatments and untreated control. Seeding with 12.5, 25 and 50 cm inter-row spacing was included as a second factor in the experiment. Weed and crop density were measured directly after treatment. Weed and crop biomass, oilseed rape root diameter and nitrogen uptake were assessed before winter. Seed yield was measured at harvest with a plot combine harvester. In 2021, hoeing in 50 cm inter-row spacing achieved equal weed control efficacy as the herbicide treatment (65–75%). In 2022, hoeing did not reduce weed density, probably due to heavy rainfalls during the first and second pass of hoeing. However, herbicide treatments controlled 92% of the weeds. In 2021, hoeing significantly increased oilseed rape shoot biomass and root diameter compared to the herbicide treatment and the untreated control. Hoeing in 50 cm inter-row spacing resulted in equal yield as the herbicide treatment. In 2022, weed control treatments and inter-row spacing had no effect on oilseed rape biomass, root diameter and yield. This study shows the potential and limitations of weed hoeing in oilseed rape. It also underlines the strong ability of modern oilseed rape cultivars to suppress annual broadleaved weeds.

To address the issue of low soil nutrients and low crop yields in coastal alkaline salines, a field experiment of straw combined with plastic film mulching in coastal alkaline salines was conducted in this study to explore the effects of different treatments on soil nutrients, enzyme activities and maize yield. Four treatments, including no mulching (NM), straw mulching (SM), plastic film mulching (PM), and straw mulching combined with plastic film mulching (SP), were set up during 2019–2020. In the 0–20 cm soil layer, compared with NM, the soil organic carbon (SOC) and soil catalase activity (SCA) of SM significantly increased by 23.4% and 46.2%, respectively (P < 0.05). The soil total nitrogen (STN), soil available phosphorus (SAP), available potassium (SAK), sucrase activity, urease activity, alkaline phosphatase activity, and maize yield (MY) of SP significantly increased by 40.7, 26.8, 13.9, 34.6, 73.8, 36.2 and 19.0%, respectively (P < 0.05). SOC, STN, SAP, SAK and SCA were significantly correlated with MY. Therefore, straw mulching combined with plastic film mulching has the best effect on increasing soil nutrients, soil enzyme activity, and maize yield and is suitable for promotion and application in coastal alkaline salines.

Peanut is a plant characterised by belowground fruiting that absorbs nutrients not only through its roots but also through its pods. However, little is currently known regarding the species of bacteria that contribute to nutrient absorption and utilisation in this plant’s pod and root zones. This study examined the effects of root and pod area nitrogen (N) fertiliser application on peanut rhizosphere and geocarposphere microbial communities and functions. Using two peanut cultivars [nodulated Huayu 22 (H) and non-nodulated NN-1 (B)], we applied the following four treatments: no N fertiliser (HT1, BH1); N applied to geocarposphere soil (HT2, BT2); N applied to rhizosphere soil (HT3, BT3), and N applied to both rhizosphere and geocarposphere soil (HT4, BT4). The results revealed that compared with HT1 and BT1, the HT3, HT4, BT3, and BT4 treatments promoted increases in total plant accumulated N of 11.2, 30.1, 38.5, and 9.9%, respectively. Moreover, N input contributed to an increase in the abundance of bacteria colonising the surrounding pods, which differed significantly from bacteria colonising the rhizosphere. Among the top four bacterial phyla detected, we recorded a significant increase in the relative abundances of Proteobacteria and Gemmatimonadetes in response to treatments HT2 and HT4, whereas the highest relative abundances of Acidobacteria and Actinobacteria were detected in HT3 plants. Regarding cultivar B, we detected increases in the relative abundances of Bacteroidetes and Gemmatimonadetes in response to the BT2 and BT4 treatments, and in the relative abundance of Actinobacteria in BT3 treated soil. The findings of FAPROTAX functional analysis revealed clear differences among the T2, T4, and T3 treatments of two peanut cultivars concerning the functional groups with the highest relative abundances. These findings will make a considerable contribution to enhancing our understanding of the effects of N fertilisation on soil microbial structure and function in the rhizosphere and geocarposphere of peanuts and can provide a basis for identifying beneficial bacteria for promoting N utilisation and yield enhancement.

This work discusses the differences in the removal efficiency of heavy metals in soils along the China-Russia crude oil pipeline in different vegetation backgrounds. In this paper, two representative forest types, birch forest and larch forest, were selected for replicated sampling and experimental study in the soil of disturbed and undisturbed areas along the pipeline, respectively. The results showed that after ten years of vegetation restoration, the amount of heavy metals in the soil of birch and larch forests decreased, the Cu content in the soil under the background of the birch forest was higher than that of the larch forest, while the Zn, Mn and Pb contents were lower than that of the larch forest. The order of decreasing magnitude was Mn, Pb, Zn and Cu, and the overall decreasing rate of heavy metal content in larch forest soil was more obvious. The above conclusions indicate that vegetation restoration is an effective measure to alleviate soil heavy metal pollution.

The aim of this trial was to verify the influence of various autumn-applied nitrogen fertilisers on the growth, yield and quality of winter oilseed rape. In the three years, small-plot field trials were carried out at the Research Station Červený Újezd (50.0697044N, 14.1659086E). The hybrid cultivar DK Exstorm was chosen, with a sowing rate of 50 seeds/m2. Five fertilisation regimes were tested: (1) nitrogen-free control; (2) CAN (calcium ammonium nitrate); (3) ANU (ammonium nitrate urea); (4) U (urea), and (5) US (urea with N-(n-butyl)thiophosphoric acid triamide (NBPT) inhibitor). A uniform dose of 40 kg N/ha was applied at the end of October. Fertilisers U (leaf length, root collar diameter, leaf and root dry weight) and US (number of leaves and root length) had the best growth outcomes. The highest seed yields were obtained with US (5.83 t/ha) and ANU (5.82 t/ha) applications, which outperformed the unfertilised control by 0.65 and 0.64 t/ha, respectively. CAN fertiliser appears to be unsuitable for autumn fertilisation in terms of yield. There were no statistically significant differences in oil content (%) or thousand seed weight (g) between the treatments in any of the experimental years.

In this study, Illumina MiSeq sequencing of 16S and ITS2 rRNA genes were used to determine the dynamic changes in bacterial and fungal communities and soil properties and enzyme activities in rhizosphere soil of ginseng under forest after 5, 10 and 15 years of cultivation and different growth stages. Results showed that the changes were particularly prominent in 10-year-old ginseng under forest, and the trends of organic carbon, alkaline hydrolysed nitrogen, and available potassium were extremely similar in different duration of the crop, especially in the middle stage of rapid root growth, when soil nutrient consumption was severe, and soil enzyme activities of rhizosphere were significantly reduced. The observed changes in soil properties and enzyme activities caused by the cultivation duration of the crop and growth stage could be explained by the variations in the microbiome. The microbial composition of 10-year-old ginseng under forest has undergone significant changes, at the genus level, both Acinetobacter bacteria and Kazachstania fungi exhibited a higher abundance; the abundance of Bacillota (Firmicutes), and Candidatus udaeobacter with significantly lower abundance. This study initially revealed the changes in nutrient utilisation of ginseng under forest at different cultivation duration of the crop and different growth stages, as well as the regulatory role played by microbes in this process preliminarily. We consider 10 years to be a critical stage for the long-term cultivation of ginseng in the forest, during which it is more sensitive to environmental factors and may exhibit special dynamic changes affecting its growth and quality. This provides a reference for further precision planting and harvesting of ginseng under the forest.

Deep placement of controlled-release urea is an effective fertiliser management strategy for improving the maize productivity, but it is not clear whether and how controlled-release urea depth affects the stem and root lodging of spring maize. Two consecutive years of field experiments were conducted to elucidate stem and root lodging properties and their relationship between grain yield and lodging behaviours under various controlled-release urea placement depths. Results depicted that compared to broadcast nitrogen treatment (D0), deep controlled-release urea significantly decreased the stem lodging rate by 34.7–80.4%, which contributed to improving the mechanical characteristics of the internode by optimising the internode diameter and dry matter in the third basal internode as well as higher lignin content. In addition, due to a greater and deeper root system (root dry weight, root surface area, root length and root width) as well as larger angle, diameter, and tension of aerial root that significantly decreased root lodging rate (37.0–88.4%). Furthermore, deep placement of controlled-release urea significantly increased the 100-grain weight, grain number and harvested index by constructing a deeper and larger root system, which significantly improved maize grain yield by 14.2–38.5%, and the nitrogen use efficiency increased by 4.8–10.7%. The highest grain yield, nitrogen use efficiency and lowest lodging rate occurred in controlled-release urea placement depths of 15 cm. Hence, our study suggests that controlled-release urea placement depths of 15 cm were an efficient nitrogen fertiliser management strategy to improve crop productivity as well as lodging resistance in spring maize.

Low water availability is a significant constraint on global crop production. Exploration is needed regarding plant responses to drought in interaction with biochar, encompassing optimised water use and carbon allocation strategies. The size of the biochar particles also plays an important role, especially in influencing the dynamics of water and plant growth. This study explored the potential impact of biochar treatment on radish growth and drought tolerance. Finer biochar particles lead to the most substantial available water content for plants, increasing at around 30%, while medium and larger fractions increase by about 22% and 16%, respectively, compared to control soil. The chlorophyll fluorescence technique showed improved water management of drought stress at larger fractions of biochar. Our research underscores the potential of biochar treatments for environmental stresses and water scarcity in modern agriculture.

Clarifying the effects of meteorological factors on the growth and development of fresh maize after delayed sowing is important for selecting appropriate sowing dates and improving yield. Six sowing dates (B1 (March 10); B2 (March 20); B3 (March 30); B4 (April 9); B5 (April 19), and B6 (April 29)) and three fresh maize cultivars (A1 (Wan Nuo 2000); A2 (Nongke Nuo 336), and A3 (Caitian Nuo 6)) were chosen for experiments conducted between 2021 and 2022 in Guiyang, Qingzhen City, China. The results showed that the whole growth period and sowing-silking period were significantly reduced with delayed sowing, while the grain-filling period was relatively stable. Delayed sowing was beneficial in increasing the number of endosperm cells and the weight of the hundred kernels. The graining filling rate and the activities of four key starch synthesis enzymes (sucrose synthase, ADP-glucose pyrophosphorylase, starch branching enzyme, and starch debranching enzyme) were significantly influenced by light, temperature, and precipitation, and they mainly affected the hundred kernel weight. The yield tended to increase with delayed sowing, and the correlation analysis between precipitation and yield at different sowing periods showed a significant effect of precipitation on yield. Delaying the sowing to mid-early April was more favourable for grain filling, enhanced key enzyme activity, and increased the kernel weight and yield. These results highlight the importance of choosing excellent cultivars and matching them with the most suitable sowing date to fully exploit climatic resources and achieve high-yield and high-efficiency cultivation of fresh maize.

Coastal wetlands play a vital role in the migration and transformation of heavy metal pollutants in watersheds. There were 30 surface sediment samples that were analysed to investigate the distribution and ecological risks of heavy metals in the coastal wetlands around the Bohai Sea. Our results showed that the average concentrations of Pb, Cr, Ni, Cu, Zn, and Cd in these wetlands were 17.92 ± 5.81, 50.29 ± 20.50, 31.53 ± 9.71, 25.37 ± 4.29, 80.13 ± 15.11, and 0.92 ± 0.54 mg/kg, respectively. Relative to other wetlands, Pb (25.43 ± 2.68 mg/kg) and Cd (1.67 ± 0.06 mg/kg) contents were higher in the Liaohe Delta wetland (LHDW). Cu (28.44 ± 3.71 mg/kg), Cr (83.11 ± 5.80 mg/kg), and Ni (45.91 ± 3.02 mg/kg) contents were higher in the Yellow River Delta wetland (YRDW). The Zn (120.86 ± 7.41 mg/kg) content was higher in the Qilihai wetland. Heavy metal concentrations in coastal wetland sediments are shown to be positively correlated with organic matter content. Our results showed that the concentration of heavy metals decreases with increasing sediment particle size. In this study, Cd showed the highest pollution index and, therefore, more attention should be paid to the potential ecological risks of Cd in coastal wetlands around the Bohai Sea, especially in the LHDW and YRDW.

Today, there is widespread concern about the potential health effects on populations from consuming contaminated leafy plants and vegetables. In this study, heavy metal content is present in commonly consumed leafy plants (Atriplex hortensis, Spinacia oleracea, Urtica dioica, Beta vulgaris, and Brassica oleracea) from the mining area near Tuzla in Bosnia and Herzegovina was determined. After the preparation of the samples by wet digestion with HNO3, the flame and graphite furnace atomic absorption spectrometry was used. According to the results, the lowest concentration in leafy plants was obtained for Cr 0.08 mg/kg (Brassica oleracea) and the highest for Fe 539.15 mg/kg (Spinacia oleracea). The novelty of this study was to estimate health risk assessment for selected leafy plants. The estimated daily intake (EDI) of Pb, Mn, Zn, and Cd from consuming leafy plants was higher than the maximum tolerated daily intake. For adults, the total target hazard quotient (THQ) calculated based on EDI of the heavy metals was found to be > 1 for Pb and Cd due to all leafy plant consumption and for the children risk level of THQ was observed for most heavy metals. The hazard index due to the intake of toxic metals from ingesting leafy plants was much > 1. According to the total carcinogenic risk index for adults and children, the carcinogenic risks for all samples were higher than the tolerable range. Based on the results of this study, there is a significant non-carcinogenic and carcinogenic health risk to the population associated with the consumption of leafy plants cultivated in the mining area.

Partial slow-release fertiliser substitution for urea combined with water-saving irrigation may synergistically improve rice yield, quality, water, and nitrogen (N) utilisation. A field experiment to evaluate different combinations of irrigation regimes: alternate wetting and drying irrigation (AWD) and flooding irrigation (FI), and N strategies: N0 (no N fertiliser); N1 (100% conventional fertiliser); N2 (100% SCF – sulphur-coated fertiliser); N3 (70% SCF + 30% urea), and N4 (50% SCF + 50% urea) on efficient rice production. Results indicated that higher substitution rates of SCF (N2 and N3) increased total N and ammonia N in surface water, leachate, and soil while reducing nitrate N relative to N1. The N3 strategy showed the highest yields, dry matter, total N uptake, and water N utilisation due to a nutrient release pattern that matched rice growth requirements. AWD yielded 5% lower than FI, except for the N3 strategy, but protein content increased by 12%, and amylose content dropped by 17%. The structural equation model analysis suggested that SCF positively impacted yield by influencing surface water total N and soil total N. Our findings indicate that implementing AWD alongside a 70% SCF basal fertiliser and 30% urea topdressing can optimise rice yield and quality while effectively managing water and fertiliser resources in the middle-lower Yangtze River Basin.

The impact of toxic elements (TEs) contaminating the root zone of Lupinus angustifolius L. on enzymatic activities, nitrification rate, and changes in the root system was evaluated. Lupine was cultivated in a pot experiment using two types of soil – control and contamination (with a high degree of arsenic (As), cadmium (Cd), lead (Pb), and zinc (Zn) contamination). After harvesting lupine biomass, enzyme activities (β-glucosidase, acid phosphatase, arylsulphatase, lipase, chitinase, cellobiohydrolase, alanine aminopeptidase, and leucine aminopeptidase) in soils were analysed. Enzyme activities decreased with TE soil contamination. According to our results, arylsulphatase was found to be the most sensitive soil enzyme to TEs. The nitrification rate is closely related to soil contamination and plant activity, as it stimulates microbial growth and multiplication through root exudates. The close correlations confirmed this relationship (r = 0.73−0.99). An increasing trend in TE contents in the roots was observed with soil contamination. Plant hormones are crucial in regulating root growth and development under stress conditions. The levels of determined phytohormones in our experiment (auxins, abscisic acid (ABA), salicylic acid (SA), and bioactive cytokinins (bCKs)) were lower in the contamination compared to the control. Correlations confirmed a significant negative relationship between the TE content in the roots and the contents of phytohormones (auxins: r = –0.96 to –0.97; ABA: r = –0.83 to –0.86; SA: r = –0.95 to –0.99, bCKs: r = –0.87 to –0.93). The ratios of these hormones (not their absolute values) appear to be the determining factor for regulating root development and protecting plants from oxidative stress.

A suitable irrigation pattern is of great significance for reducing greenhouse gas emissions. In this study, field experiments and a denitrification-decomposition (DNDC) model were used to study the global warming potential based on CH₄, N₂O and CO₂ fluxes under flooding irrigation and controlled irrigation in paddy fields in the Erhai Lake basin. The results showed that the average value of CH₄ flux under controlled irrigation was lower than that under flooding irrigation, with a reduction range of 43.21% to 48.88%, however, the average value of the N₂O and CO₂ fluxes from paddy field under controlled irrigation were higher than those under flooding irrigation. Controlled irrigation patterns can significantly reduce the global warming potential in paddy fields based on CH₄, N₂O and CO₂ fluxes. Controlled irrigation can effectively reduce the global warming potential per unit yield. For water management in the Erhai Lake basin, it is recommended the controlled irrigation treatment of soil moisture with an upper limit of 100% and a lower limit of 75–85% with irrigation, and a maximum surface water depth of 150–200 mm lasting for five days after precipitation from the jointing-booting stage to the milk stage.

Summer maize cultivars are differently sensitive to soil moisture. To better understand the differences in water productivity of summer maize cultivars with different water sensitivity, a field experiment was conducted from 2020 to 2022. Three different water-sensitive summer maize cultivars were selected, including TY808 (high water-sensitive cultivar), DH605 (medium water-sensitive cultivar), and ZD958 (low water-sensitive cultivar). Soil water content (SWC), soil water storage (SWS), water consumption, water use efficiency, and grain yield were determined. The results showed that under rainfed conditions, the SWC of the medium water-sensitive cultivar DH605 in the deep soil layer was 2.1–18.2% lower than TY808 and ZD958, respectively, and the differences were significant in the 12th leaf stage (V12) and vegetative tassel stage (VT). The SWS of the high-water-sensitive cultivar TY808 was 0.7% to 6.4% higher than the other two water-sensitive cultivars from 2020 to 2022. The changes in SWS are related to the spatiotemporal distribution of precipitation. The water consumption of DH605 was higher than TY808 and ZD958 by 5.3% and 7.09% in 2020 and 2.9% and 2.8% in 2021; in 2022, DH605 is 2% higher than ZD958 and 2.8% lower than TY808, respectively. The yield of DH605 was 4.3–10.78% higher than the other two cultivars in the three-year experiment. Additionally, the 1 000-kernel weight of DH605 was the highest in TY808 and ZD958. DH605 has the highest water use efficiency, which was increased by 4.8–14.6% compared to TY808 and ZD958. Through path analysis, we found that the direct path coefficient of SWS in the VT stage on yield reached 0.999, indicating that soil moisture in the VT stage has the greatest impact on yield, followed by the blister stage (R2). In conclusion, our results suggest that the water consumption of summer maize during the VT stage is the highest, and the soil moisture condition in VT significantly affects the grain yield of summer. Planting DH605 in the North China Plain would harvest the maximum grain yield and water productivity.

We investigated, under field conditions and during four years (2018–2021) the effects of five irrigation levels (T1: 100% of crop water requirement; T2: 80% of T1; T3: 60% of T1; T4: 40% of T1, and T5: 0% of T1 – rainfed) in interaction with three planting densities (PD1: 54 900, PD2: 64 900, and PD3 75 200 plants/ha) on the yield, yield components and water use efficiency (WUE) of maize in Srem, Serbia. The results indicate a large year-to-year variability, mainly due to the total amount and distribution of rainfall. Water regime and PD interacted significantly. Irrigation increased grain yield 28, 34, 30 and 18% for treatments T1, T2, T3 and T4, respectively, compared to the T5; and significantly influenced the yield components. Planting density had significantly lower effects on grain yield compared to irrigation (+1.4–1.8%). WUE is maximised (3.436 kg/m3) at T4 under 75 200 plants/ha. Grain yield and WUE increased significantly with increasing PD, while the number of grains per ear and the weight of 1 000 grains decreased with increasing PD. In conclusion, limited irrigation at T2 under PD2 may be a viable method to maximise production efficiency and maize yield under the environmental conditions of this study and at sites with similar soil and climatic conditions.

Changing political demands requires the search for alternatives to glyphosate, which has often been used in the stale seedbed method. In three field trials between 2020 and 2023, three electrophysical treatments (XPower System, Zasso®) differing in speed, three mechanical treatments (disc harrow, rotary harrow, cultivator) and three chemical treatments (glyphosate, maleic hydrazide, pelargonic acid + maleic hydrazide) were evaluated concerning the efficacy and economic performance in stale seedbed method in Alopecurus myosuroides control. Process costs for each treatment were calculated. Furthermore, the maximum investment costs for the XPower system were calculated to be on the same level as the other treatments. In all treatments, the density of A. myosuroides in autumn was significantly lower than in the control. In general, the fewest plants and heads were found in the chemical treatments. Despite the lower plant density, the electrophysical method did not show a significant difference in the number of heads compared to the untreated control, especially at higher speeds. Calculated process costs ranged between
40 €/ha (glyphosate) and 430 €/ha (pelargonic acid + maleic hydrazide). To be economically comparable with the other treatments, the investment of the XPower should be at maximum between – 219 000 € (glyphosate) and 300 000 € (pelargonic acid + maleic acid), depending on the driving speed. In a holistic view, electrophysical control, as well as pelargonic acid and maleic hydrazide as glyphosate alternatives in the stale seedbed method, are not suitable. A stale seedbed with mechanical control of A. myosuroides is recommended.

In this study, a synergist made of itaconic acid, maleic acid, acrylic acid and other active ingredients polymerised was sprayed on the surface of nitrogen (N) fertiliser particles to make synergistic nitrogen fertilisers (SNF). To explore the effect of SNF on N metabolism of wheat in saline-alkaline land, five treatments were set up: CK – ordinary N fertiliser (299.86 kg N/ha); T1 – SNF (299.86 kg N/ha); T2 – SNF (239.89 kg N/ha); T3 – SNF (179.92 kg N/ha); T4 – SNF (119.94 kg N/ha). The aboveground dry weight of wheat, the photosynthetic characteristics of wheat flag leaves, the activity of the N metabolism enzyme of wheat flag leaves, the expression of N transporter-related genes in wheat roots, and the N accumulation and transport of plants were determined. The results showed that the T1 treatment performed the best. During the two years, the N translocation from stems and leaves to spikes of plants at maturity in T1 was 33.18–45.55% higher than that of CK. The N content of wheat spikes was 12.01–12.66% higher than that of CK. The activities of nitrate reductase, glutamine synthetase, glutamate synthetase and the expression of nitrate transporter gene TaNRT1.1 and ammonium transporter gene TaAMT1.1 were significantly higher than that of CK. The aboveground dry weight of wheat and photosynthetic characteristics of flag leaves were significantly higher than those of CK in T1, whereas the intercellular CO2 concentration was significantly lower than that of CK. The application of SNF positively affected N accumulation and transport in wheat, wheat yield, and fertiliser utilisation, as well as reduced N loss in saline-alkaline land.