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Water deficit is one of the most important abiotic factors limiting crop yields. To better understand the link between carbohydrate balance and drought stress response in strawberry plants (Fragaria vesca), we monitored by liquid chromatography the carbohydrate status in leaves during exposure to drought of different duration and intensity as well as subsequent recovery. In two greenhouse experiments that differed in the rate of reaching the target water deficit, strawberry leaves showed osmotic adjustment, with gradual increases in glucose and fructose content, likely provided by observed starch degradation. At the point of the most severe stress, proline content increased, while stress markers, such as malondialdehyde content and chlorophyll fluorescence, showed no significant changes. It indicates the defence mechanisms’ ability to protect cellular structures effectively. Strawberry, a member of the Rosaceae family, motivated us to investigate the role of sorbitol in the stress response. However, we found no sorbitol in any stress or control situations. Finally, testing sorbitol’s ability to support strawberry plant or non-green callus growth in vitro did not indicate that sorbitol could be used as a carbon and energy source. In conclusion, strawberries exhibit marked changes in soluble carbohydrate and starch content as an efficient defence against drought, without apparent involvement of sorbitol.

Soil available and total zinc are important indicators of soil zinc status, yet the global effects of different fertilisation practices on soil available and total zinc and the drivers of their variation remain insufficiently quantified. We conducted a global meta-analysis based on data extracted from published field studies. A total of 1 240 paired observations of soil available zinc from 94 studies and 364 paired observations of soil total zinc from 44 studies published between 1993 and 2024 were compiled. The effects of eight fertiliser types (mineral fertilisers without zinc [CF], compost, manure, zinc fertiliser, CF combined with either compost [CFC] or manure [CFM] or zinc fertiliser [CFZn], and compost combined with zinc fertiliser [CZn]) on the soil available and total zinc content were assessed by meta-analysis. The results indicated that compared to the control group, soil available zinc content increased significantly under treatments CZn, CFZn, zinc fertiliser, CFM, manure, CFC, and compost by 158, 134, 133, 84, 78, 43, and 35%, respectively. Additionally, manure, CFM, zinc fertiliser, CFZn, and CZn treatments significantly enhanced soil total zinc content, with increases ranging from 25% to 32%. Applying zinc fertiliser at > 20 kg Zn/ha significantly increased soil-available zinc. In the medium-rate CZn class (10–20 kg Zn/ha), soil available zinc increased from 0.78 mg/kg in the control soils to 3.46 mg/kg in the treated soils. Among crop systems, wheat showed a stronger response in soil-available zinc, whereas rice-growing systems showed relatively larger increases in soil-total zinc under manure and CFM treatments. Fertilisation intensity, crop types, soil organic carbon, and soil pH emerged as key drivers of variation in soil available zinc, whereas the main drivers of soil total zinc varied among fertiliser types and were more often associated with fertiliser rate and crop types. When soil organic carbon was ≤ 12 g/kg or soil pH was > 7.5, applying CZn at 10–20 kg Zn/ha showed greater potential to increase soil available zinc. These findings suggest that soil zinc management should be optimised based on fertilisation intensity, crop type, soil organic carbon, and soil pH to improve zinc availability while avoiding excessive accumulation.

The effect of mineral, organic (manure or straw + intercrop) and combined fertilisation on the development of soil nutrient contents over time and their mutual ratios was evaluated in a long-term field trial, IOSDV (established in 1984 at two sites), differing in the soil-climatic conditions. Three cropping cycles, from 2016 to 2018, 2019 to 2021, and 2022 to 2024, were studied in the following crop rotation: winter wheat-winter barley-root crop (sugar beet at Ivanovice na Hané and potatoes at Lukavec). Potassium and phosphorus in mineral fertilisers have not been applied since the year 2020 due to their high content found in soils after dry years with low yields. Consequently, their content decreased, most in the third rotation, both by the Mehlich 3 method and especially the exchange fraction extractable with NH₄-acetate (Ivanovice: P 5–14%, 32–40% and K up to 12%, 9–20% determined by Mehlich 3 and NH₄-acetate, respectively; Lukavec: Mehlich 3 – P increase: 5–16%, K decrease: 0–8%; NH₄^- acetate decrease – P: 10–13%, K 8–23%). The mutual ratio of nutrients equivalents K : Mg : Ca was lower than required values 1 : 2–3 : 10–15 at both sites and all studied treatments, however a slight increase was observed during the studied period, above all in system with only mineral fertilisation (Ivanovice: K : Mg : Ca from 1 : 1.2 : 5.6 to 1 : 1.4 : 6.8, Lukavec: from 1 : 1.0 : 7.7 to 1 : 1.0 : 9.6). A correctly balanced ratio of nutrients in the soil is important for maintaining soil fertility. In this long-term field experiment, the increase in nutrient levels in soils over reasonable levels was observed, highlighting the necessity of regular nutrient testing in agricultural soils, especially when multiple types of fertilisers are used simultaneously.

Combining phosphorus management with phosphorus-efficient cultivars is an effective strategy for improving rice quality. To investigate their effects on root characteristics and photosynthetic traits, a pot experiment was conducted with two rice cultivars differing in phosphorus efficient: Liangeng 7 (weakly efficient) and Yongyou 2640 (highly efficient). Four phosphorus rates (0, 0.44, 0.88, and 1.32 g/pot, designated as P0, P1, P2, and P3, respectively) were applied. A significant cultivar-phosphorus interaction was observed. Most root traits (the length, dry weight, volume, total absorption area, active absorption area, oxidation activity, and acid phosphatase activity) and photosynthetic traits (photosynthetic rate, transpiration rate, and stomatal conductance) initially increased and then decreased with increasing phosphorus rates, while the leaf intercellular CO₂ concentration showed the opposite trend. Liangeng 7 performed optimally under P2, whereas Yongyou 2640 reached its peak under P1. Compared with Liangeng 7, Yongyou 2640 exhibited better appearance quality, root traits, and photosynthetic parameters. Correlation analysis showed that root length, root physiological activity and leaf photosynthetic parameters (except intercellular CO₂ concentration) were significantly negatively correlated with chalkiness degree. These findings demonstrate that matching phosphorus supply to cultivar‑specific efficiency optimises root‑photosynthesis synergy, leading to superior grain appearance quality with less phosphorus input.

Salinity stress poses an increasing threat to global rice production, particularly under climate change. Enhancing salinity tolerance is crucial to sustain rice production and food security. This study aimed to assess genetic variation among rice parental genotypes and their derived crosses under salinity stress by evaluating physiological, biochemical, agronomic, and yield-related traits. Seven diverse rice genotypes were used to develop 21 crosses using a half-diallel mating design in the summer of 2023. The parental genotypes and their derived crosses were evaluated in the summer of 2024 under controlled greenhouse lysimeter conditions. Salinity stress was induced by irrigation with water containing 10.60 dS/m, and soil salinity was maintained at 9.60 dS/m through controlled irrigation and drainage. Twenty key traits were studied, including phenological and agronomic attributes, yield traits, and physiological and biochemical markers such as relative water content, leaf CO₂ assimilation, proline accumulation, malondialdehyde content, and antioxidant enzyme activities, to assess salinity tolerance in rice genotypes. The results demonstrated highly significant variation among the evaluated parental genotypes and their derived crosses across physiological, biochemical, agronomic, and yield-related traits, indicating considerable genetic variability in the studied plant materials. The genotypes C9, R8, and R6 were identified as superior combiners contributing favourable alleles for salinity tolerance. Eleven promising F1 crosses exhibited enhanced growth, improved antioxidant enzyme activities, osmotic adjustment, reduced oxidative damage, and higher grain yield under salinity stress. Exploiting these plant materials can improve the development of novel rice genotypes tolerant of salt-affected environments, addressing the current challenges posed by climate change. Strong associations were observed among physiological, biochemical, agronomic, and yield-related traits, indicating an integrated network of responses that collectively contribute to enhanced salinity tolerance in rice.

This study investigated how wheat (C3) and maize (C4) respond to soil thallium (Tl) contamination and elevated CO₂ (eCO₂), aiming to understand strategies for mitigating oxidative stress. Under eCO₂, both crops showed higher biomass production. However, high Tl concentration (120 mg/kg) significantly decreased fresh and dry weights by 31–59%, which translated directly to compromised yield. This growth decline is linked to impaired photosynthesis, evidenced by a 54–57% drop in net photosynthetic rate under elevated Tl. Such photosynthetic inhibition intensifies oxidative stress, marked by increased membrane damage and hydrogen peroxide (H2O2). Furthermore, photorespiration contributed to oxidative stress by generating H₂O₂, with increased activities of glycolate oxidase and hydroxypyruvate reductase rising by 122% and 201%, in wheat and by 179% and 39% in maize, respectively, in response to 120 mg/kg TI under eCO₂ conditions. Simultaneously, to mitigate oxidative damage, antioxidant defences were significantly enhanced, resulting in increased activity of the ascorbate (ASC)/glutathione (GSH) cycle, along with elevated levels of metallothionein and phytochelatin for Tl sequestration, as well as augmented glutathione S-transferase activity. Overall, findings reveal complex interactions between CO₂ and Tl, highlighting species-specific adaptive responses of C3 and C4 plants. C3 plants use photorespiration to combat oxidative stress, while C3 and C4 plants have strong antioxidant systems to reduce the effects of oxidative stress, promoting crop resilience and growth despite Tl toxicity.

Effective weed management is crucial in the critical period of sugar beet production, but often lacks sustainability and environmental protection. Recent advancements in sensor-based weed control systems have rendered the latter a realistic prospect, which demands detailed analyses, especially under suboptimal field conditions. The present study analysed six robotic-assisted weed control systems (RAWS) in three experiments on sugar beets in 2024, conducted under dry soil and high weed pressure. The experiments included sensor-based inter-row and intra-row hoeing, spot- and band-spraying and were compared to a broadcast herbicide treatment and an untreated control. Weed control efficacy (WCE) in the intra- and inter-row areas, as well as weed species composition and crop plant damage, were assessed after treatment. The data show that intra-row WCE of two hoeing robots (Farming GT® and Robovator®) equipped with selective intra-row blades achieved up to 80%, which was higher than the broadcast herbicide control with 67% WCE. In the inter-row area, Farming GT® robotic hoeing and ARA® spot-spraying resulted in more than 90% WCE, which was equal to the broadcast herbicide application. Weed species composition was not affected by the different RAWS. Crop plants were affected by all hoeing treatments with maximum non-lethal burial rates of 33%. The highest lethal uprooting of crop plants occurred after Farming GT® robotic hoeing, at 5.5% overall. The results demonstrate the great potential of robotic weeding to replace broadcast herbicide applications.

The species diversity of the unique flora in the Hrubý Jeseník Mountains is currently threatened due to the absence of traditional grazing, which was historically used as a management practice. This study evaluates changes in floristic composition in areas near the Švýcárna and Ovčárna lodges, where cattle and sheep grazing was reintroduced in 2012 and 2014, respectively, after long-term abandonment. The floristic composition was assessed using permanent plots and analysed statistically. In total, 84 plant species were recorded in the Švýcárna experimental area over 12 years. All experimental plots throughout the study observed an increase in species richness. In the Ovčárna area, a similar trend was detected, particularly in grazed grasslands dominated by Avenella flexuosa, Festuca supina, and Ligusticum mutellina. The reintroduction of grazing in these areas serves not only as a symbolic return to traditional land use but primarily as an effective management tool to suppress ecological succession and maintain or enhance plant species diversity in biologically valuable habitats.

Hemp is becoming increasingly popular, and many new varieties are coming onto the market to meet the requirements of different industries. In this study, the seed and stem yield, seed nutritional properties and the biochemical characteristics of the inflorescences of seven European varieties (Fedora 17, Futura 75, KC Dóra, Monoica, Santhica 27, Tiborszallasi, USO 31) were investigated in a 3-year field trial. Futura 75 and Tiborszallasi stand out as varieties with the highest potential in the conditions of the experiment (humid continental climate with oceanic influences, heavy soil). Futura 75 achieved the highest seed yield (505 kg/ha dry matter), stem yield (8 036 kg/ha fresh matter), protein yield (140 kg/ha) and oil yield (181 kg/ha). There were no differences in protein content (average 21.0%) among varieties. The total unsaturated fatty acid content was as high as 87.6% at Tiborszallasi. The best ratio between omega-6 and omega-3 fatty acids was 3 : 1 in Tiborszallasi, which had also the highest oil content (30.2%), the highest total phenolic content (2.8 mg caffeic acid (CA)/g) and the best antioxidant potential (6.69 EC50 DPPH (2,2-diphenyl-1-picrylhydrazyl) mg/L). Most varieties had higher cannabidiol and tetrahydrocannabinol contents in the inflorescence at seed maturity (from 0.22 to 3.3 for cannabidiol (CBD) and from 0.00 to 0.32 for tetrahydrocannabinol (THC)) compared to full flowering (from 0.17 to 4.33 for CBD and from 0.00 to 0.52 for THC, on average 2.64% for CBD and 0.19% for THC), presenting an opportunity for dual-purpose use.

Crop production faces increased climate change and land degradation stresses, compromising global food security with the growing population. Maize (Zea mays L.) is a versatile crop used for food, feed, and raw materials, contributing significantly to global food systems. Abiotic stresses like drought and soil fertility limit its production. Fertilisation is an amelioration technique that optimises maize growth and yield by maintaining optimum nutrition and leveraging nutrient deficiency conditions. Precision agricultural tools like chlorophyll meters are essential for non-destructive chlorophyll assessment and nitrogen status. An experiment conducted at the University of Debrecen evaluated the impact of nitrogen (N) fertilisation (0, 90, and 150 kg/ha) and three maize cultivars (P9610-FAO 340, DKC4590-FAO360, and GKT376-FAO360) on physiological parameters, namely: relative chlorophyll content (SPAD), normalised differences vegetation index (NDVI) and grain quality. Results showed that SPAD and NDVI positively correlated (P < 0.05) with grain quality and yield. Nitrogen application significantly influenced SPAD. Maize cultivars and N rates with higher chlorophyll content had maximum yield. Cultivar responses to nitrogen rates significantly (P < 0.05) varied by crop year. Higher SPAD and NDVI values were associated with higher protein content. Therefore, SPAD and NDVI values could be used to analyse the nutrient requirements of maize under field conditions to estimate grain yield.

Although the effects of fertiliser addition and liming on semi-natural grassland productivity and biomass quality are well documented, less is known about how fertilisers change plant functional groups and mean ecological values. We researched the effects of liming (no lime and lime with 1 t/ha) and mineral fertilisers (control – no fertilisers, PK-P60K60, N20PK-N20P60K60, N80PK-N80P60K60, and N140PK-N140P60K60) for nine years on the Danthonia alpina Vest. grassland community. Based on Brown-Blanquet cover abundance, we calculated Shannon-Wiener evenness and abundance of plant functional groups (based on height, canopy structure, storage organs presence and flowering duration). We also researched Landolt’s ecological indicator values for nutrients, moisture, reaction, light, and temperature. Results revealed that fertilisers stimulated tall species with longer flowering duration. Shannon-Wiener evenness in control was 0.45, and N20PK increased to 0.71 but significantly decreased in treatment N140PK (0.25). Mean Landolt ecological value for nutrients and moisture increased while temperature dropped. The coverage of legumes and Landolt indicator value for nutrients increased because of the lime application, while the lime had no effect on Shannon-Wiener evenness and abundance of functional groups. Greater Shannon-Wiener evenness in treatments of PK and N20PK is a prerequisite for resistance to the effects of extreme climate events.

Vietnam’s Mekong River Delta (MRD), where rice is the dominant crop, is increasingly impacted by salinity intrusion, highlighting the need for alternative cropping options. This study evaluated the growth and yield performance of quinoa, beetroot, and maize under three irrigation salinity levels (0, 2 and 4 g/L), with and without rice straw mulch (7 t/ha), in greenhouse conditions representative of the MRD dry season. Agronomic traits, physiological parameters, and changes in soil, including electrical conductivity (ECe), soluble sodium (Sol-Na⁺), and exchangeable sodium percentage (ESP), were assessed. Results showed that quinoa demonstrated the greatest salinity tolerance, maintaining stable growth and yield under 4 g/L saline irrigation and soil ECe exceeding 15 dS/m. Beetroot’s yield was not significantly different under 2 g/L saline irrigation with straw mulching. Maize was highly sensitive to salinity and environmental stress, failing to complete its growth cycle under high heat and humidity, even in non-saline conditions. Across treatments, rice straw mulching significantly reduced soil ECe, Sol-Na⁺, and ESP, and improved crop performance under saline irrigation. Overall, quinoa and beetroot, especially when combined with mulching, offer promising alternatives for dry-season cropping in saline-prone areas of the MRD. In contrast, maize cultivation requires improved soil and environmental management under such conditions.

This study investigates the effects of salt stress and gamma irradiation on growth, biochemical, and physiological responses in three sugar beet (Beta vulgaris L.) cultivars. Control plants were irrigated with fresh water (EC = 1.1 dS/m), whereas salt stress was imposed with an irrigation of 9 dS/m. Seeds were irradiated with gamma rays (0, 50, 100, 200, 400 Gy) before sowing. Exposure to salt stress reduced root yield (RY), sugar yield (SY), chlorophyll content, and antioxidant enzyme activities (catalase (CAT) and superoxide dismutase (SOD)). In contrast, oxidative damage increased, as indicated by elevated malondialdehyde (MDA) concentrations. Interestingly, salt stress enhanced sugar content, with the Eudoro cultivar showing the greatest resilience, maintaining higher RY and SY and lower MDA compared to the other cultivars. Gamma irradiation at moderate doses (50–200 Gy) alleviated the effects of salt stress, with the strongest improvements in SY observed at 100 and 200 Gy. These treatments enhanced RY, chlorophyll content, and antioxidant activities, while also improving photosynthetic efficiency (Fv/Fm) and cellular integrity. Higher doses (> 200 Gy) reduced sugar content, indicating dose-specific effects. Eudoro exhibited superior salt tolerance, maintaining higher root and sugar yields (RY, SY) and reduced oxidative damage (lower MDA) under salt stress. These findings demonstrate that gamma irradiation at optimal doses enhances salt tolerance in sugar beet, offering cultivar-specific benefits for breeding programmes in saline environments.

Magnetic fields, as a form of physical energy, exert an influence on biological activities. However, our current understanding of the impact of magnetic fields on wheat yield remains limited. In this study, our objective was to investigate the effects of magnetic field treatment of wheat plants on their yield, root growth, absorption of nitrogen and phosphorus and soil bacterial diversity. The experiments were conducted at two agricultural research stations in China, Zhengzhou and Xuchang. Plants were treated with magnetic fields of 20, 40, 60, and 80 mT induced by permanent magnets for chronic exposure. Untreated plants were considered as controls. Our result showed that soil nutrients were found to have a substantial impact on wheat nitrogen and phosphorus absorption, and wheat nitrogen and phosphorus absorption significantly affected wheat yield. The change in soil nutrient content was caused by the change in soil bacterial community diversity and abundance, and increased soil nutrients increased wheat yield. The results suggest that magnetic field treatment stimulated wheat plant growth and yield, and changed soil nutrient content through improved soil bacterial community diversity and increased soil nitrogen and phosphorous absorption.

Restoration of Arrhenatherion meadows is limited both by the lack of local seed availability in natural habitats for self-restoration purposes and the lack of information on the germination of target species in these meadows. Understanding germination strategies can optimise local seed use. This study aimed to define germination strategies for groups of species based on relevant six germination parameters: germination capacity (GC), fresh ungerminated seed (FUS), median germination time, germination velocity, germination synchrony and germination uniformity. The germination test of 23 meadow species was performed according to ISTA (International Seed Testing Association) rules. The hierarchical clustering method and PCA biplot divided the species into five groups. Based on the Kruskal-Wallis and Dunn’s test, the evaluation of six parameters in five groups showed that species such as Arrhenatherum elatius, Centaurea jacea, Plantago lanceolata, Tragopogon pratensis and Dianthus deltoides differed significantly in terms of higher GC, lower FUS and faster germination velocity than Lathyrus pratensis, Vicia angustifolia and Geranium pratense. Conversely, these three species had more synchronous germination than species such as Knautia arvensis and Briza media and expressed the shortest peak of germination period among other species. These six parameters potentially describe germination strategies across groups of species.

Enhancing sustainability in agriculture requires innovative practices that boost crop productivity while conserving natural resources. This two-season field study (2023–2025) in sandy soils of El Sadat City, Egypt, evaluated the combined effects of nano-fertilisers and seed priming on the growth and yield of sugar beet (Beta vulgaris L.). Five fertilisation regimes, ranging from 100% conventional to 100% nano-formulations, were tested under both primed and unprimed seed treatments. The results demonstrated that the integration of nano-fertilisers with seed priming significantly improved sugar yield (up to 36.1 t/ha), sucrose content (20.35%), and nitrogen use efficiency (55.1 kg sugar/kg N). Post-harvest soil analysis showed improved nutrient retention, indicating enhanced environmental performance. This approach supports climate-smart agriculture by optimising nutrient input, reducing losses, and improving soil sustainability. Our findings highlight the potential of nano-agronomic inputs to contribute to global food security under conditions of climate change.

Using biostimulants to enhance plant growth and increase yield and secondary metabolites in medicinal and aromatic plants is an important strategy to achieve sustainable agriculture. The influence of two strains of nitrogen-fixing rhizobacteria (NFB) of Azotobacter chroococcum (NFB1) and Azospirillum lipoferum (NFB2), three levels of seaweed extract (SWE; 0 (SWE1), 250 (SWE1), and 500 mg/L (SWE2)) and their interactions have been investigated on Trachyspermum ammi L. (ajwain) growth, fruit yield, and essential oil constituents for two winter seasons. Growth traits (plant height, number of branches, and fresh and dry weights) and fruit traits (umbel number, 1 000-fruit weight, and fruit yield) were improved following NFB and/or SWE applications. Leaf pigments, total phenols, carbohydrates, free amino acids, and nutrient content were also enhanced. Ajwain plants that received NFB2 soil inoculation and foliarly sprayed with SWE1 observed the highest growth and yield values. Applying this treatment resulted in 27.6% and 32.7% higher fruit yield per plant for the first and second seasons, respectively, compared to the control. The results of GC-MS revealed that γ-terpinene, p-cymene, and thymol are the major components in ajwain essential oil. All applications used changed the percentages of the main components detected in ajwain essential oil. For instance, increasing SWE level caused a reduction in γ-terpinene with an increase in thymol content. The highest conservation rate from γ-terpinene to thymol was detected in NFB2 × SWE1-treated plants, with the highest thymol content and least γ-terpinene. Azospirillum lipoferum soil inoculation with SWE1 foliar application is recommended to enhance ajwain production, in terms of fruit yield and oil quality.

This study investigates the benefits of using mine tailings (MT) to improve pea (Pisum sativum L.) growth and productivity on degraded agricultural soils in semi-arid environments. The research aims to evaluate the use of MT as an innovative soil amendment and to determine the optimal dose required to enhance the micronutrient availability of Zn, Mn, Cu and Fe without affecting soil quality. The experiment was conducted in greenhouse pots with three different soil types amended with different MT doses (control and four doses). Soil samples were collected from the Doukkala region, one of the main agricultural areas in Morocco. Pea was grown in pots and monitored for 87 days until maturity. After harvest, soil and plant samples were weighed, measured and analysed by inductively coupled plasma atomic emission spectroscopy (ICP-AES). The experiment found that moderate doses (0.2 g/kg to 1 g/kg) applied to all soil types promoted optimal pea growth by improving plant height, root and above-ground biomass and pod number. Thus, MT can act as a biostimulant. However, nutrient antagonism negatively affected growth at the highest dose (4 g/kg). Bioconcentration and translocation factors indicated efficient micronutrient uptake and biofortification, while heavy metals remained immobilised in roots, effectively eliminating toxicity risks.

Arbuscular mycorrhizal fungi (AMF) play a key role in regulating the carbon cycle in terrestrial ecosystems. However, there is little information on how AMF inoculation affects the carbon fluxes of paddy fields, which are major sources of global carbon emissions. We, therefore, designed an experiment to study the effects of AMF inoculation on methane and carbon dioxide emissions from a paddy field. Results showed that: (1) Among the tested factors, the C/N ratio was the main environmental determinant of microbial community structure in the investigated soil; (2) compared with traditional fertilisation (control), the soil C/N ratio increased by 2.1~15.2% and 1.4~10.5% as a result of AMF application alone (M) or in combination with mineral fertiliser (FM) throughout the growing season, respectively. This change shifted microbial community composition to higher G⁺/G⁻ bacterial and fungal/bacterial ratios; (3) the microbial community change favoured soil carbon retention. Methane (CH₄) emission peaks were reduced by 59.4% and 76.0% versus control in the M treatment and by 52.5% and 29.4% in the FM treatment in the midseason and end-of-season drainage periods, and CO₂ emission peaks were reduced by 70.1% and 52.3% in the M plots and by 55.4% and 66.4% in the FM plots.

Agriculture faces increasing challenges due to climate change, underscoring the importance of beneficial microorganisms for enhancing crop resilience and improving soil health. However, the performance of microbial inoculant strains can vary widely depending on the cultivated species and environmental conditions. This study evaluated the ESALQ 1306 strain of Trichoderma harzianum, a soil fungus recognised as a biological control agent for crops such as soybean and strawberry, investigating its potential as a growth promoter in maize (Zea mays L.). Field experiments were conducted with three commercial cultivars (DKB255, DKB360, and 2B810) over two growing seasons, one under irrigation and the other under severe natural drought. The results revealed that Trichoderma (ESALQ 1306) significantly increased plant height, biomass, and grain yield, particularly under drought stress, despite lacking a formal recommendation for maize. The cv. DKB360 showed the greatest response, with yield increases of up to 60% compared to untreated controls. Inoculation also improved nutrient uptake, especially nitrogen, highlighting its potential to maintain soil health and fertility. These findings demonstrate that the ESALQ 1306 strain of Trichoderma is a promising soil bioinoculant for agriculture, capable of improving maize performance under both optimal and stressful conditions. However, it is important to emphasise that genotype-specific responses highlight the need to align bioinoculant application with selecting specific cultivars to ensure inoculation success. This insight is crucial for guiding future breeding programs and establishing clear regulatory guidelines for commercialising biological products, fostering sustainable and resilient agricultural systems.

Quantification of soil health dynamics relative to grazing can inform both agriculture and conservation. We conducted an experiment near Lingle, Wyoming, USA, on a semi-arid northern mixed-grass prairie from 2017–2019. Three grazing density treatments (NG – not grazed; MRG – moderate rotationally grazed a herd of 4 Angus heifers, and UHD – ultra-high density rotationally grazed a herd of 33 Angus cow-calf pairs) were replicated four times in a randomised complete block design across twelve – 0.405 ha paddocks. Soil sampling was conducted prior to grazing in June 2017, one-week post grazing in July 2019, and six weeks post grazing in August 2019 and included a suite of forage, ground cover, soil chemical, soil physical, and soil microbiological measurements. Grazing treatment did result in lower vegetation structure but had no effect on any soil variables (P > 0.05). Conversely, the sampling interval was more influential for predicting fluctuations in chemical (15 variables significantly different within at least one treatment) or microbiological (13 variables significantly different within at least one treatment) variables than grazing treatment. The study was conducted in an intact native prairie with initial and final values indicating "Very Good" soil health, including the saturated : unsaturated fatty acid ratio, an indicator of stress.

Nitrogen management and irrigation methods play crucial roles in determining rice’s grain yield and quality (Oryza sativa L.). However, limited knowledge exists on how interactions between nitrogen forms and irrigation regimes regulate starch-metabolising enzyme activity to influence rice yield and quality. A soil-growth experiment was conducted using a high-lodging-resistance rice cultivar under three irrigation methods, namely, submerged irrigation (0 kPa), alternate wetting and moderate drying (−20 kPa), and alternate wetting and severe drying (−40 kPa), as well as three nitrogen forms, namely, ammonium nitrogen (NH₄⁺-N), mixed ammonium + nitrate (50 : 50), hereafter denoted as 50 : 50, and nitrate nitrogen (NO₃^–-N). Results indicated that compared with the other treatments, alternate wetting and moderate drying interacted with 50 : 50 treatment, resulting in the following: improved grain yield by 11.7–21.0%, milling, appearance, eating and cooking, and nutritional qualities including milled-rice and gel consistency; and decreased chalky rice, chalky size, chalky degree, amylose content, and protein content by 20.0–23.1, 29.6–33.3, 44.1–48.5, 6.2–9.6 and 10.1–13.9%, respectively. The activities of adenosine phosphate glucose pyrophosphorylase (AGPase), starch synthase (SS), starch-branching enzyme (SBE), and adenosine triphosphate (ATP) enzyme in the grains also improved, with an increase of 20.0–35.0, 11.8–20.0, 13.6–26.3 and 21.2–39.6%, respectively. Conversely, severe drying and NO₃^–-N treatment negatively impacted grain yield and quality due primarily to decreased SS activity in grains under each irrigation method. Correlation analysis showed that starch-metabolising enzyme (AGPase, SS and SBE) activity at 14 days after anthesis (DAA) and 28 DAA exhibited a positive correlation with grain yield, milling quality and gel consistency, whereas negatively correlated with appearance and nutritional qualities. In summary, the adoption of alternate wetting and moderate drying and 50 : 50 interaction treatment can synergistically boost grain yield by increasing the filled-grain rate and 1 000-grain weight and enhance grain quality of rice by upregulating the activities of starch-metabolising enzyme activity.

Summer maize cultivars are differently sensitive to soil moisture. To better understand the differences in water productivity of summer maize cultivars with different water sensitivity, a field experiment was conducted from 2020 to 2022. Three different water-sensitive summer maize cultivars were selected, including TY808 (high water-sensitive cultivar), DH605 (medium water-sensitive cultivar), and ZD958 (low water-sensitive cultivar). Soil water content (SWC), soil water storage (SWS), water consumption, water use efficiency, and grain yield were determined. The results showed that under rainfed conditions, the SWC of the medium water-sensitive cultivar DH605 in the deep soil layer was 2.1–18.2% lower than TY808 and ZD958, respectively, and the differences were significant in the 12th leaf stage (V12) and vegetative tassel stage (VT). The SWS of the high-water-sensitive cultivar TY808 was 0.7% to 6.4% higher than the other two water-sensitive cultivars from 2020 to 2022. The changes in SWS are related to the spatiotemporal distribution of precipitation. The water consumption of DH605 was higher than TY808 and ZD958 by 5.3% and 7.09% in 2020 and 2.9% and 2.8% in 2021; in 2022, DH605 is 2% higher than ZD958 and 2.8% lower than TY808, respectively. The yield of DH605 was 4.3–10.78% higher than the other two cultivars in the three-year experiment. Additionally, the 1 000-kernel weight of DH605 was the highest in TY808 and ZD958. DH605 has the highest water use efficiency, which was increased by 4.8–14.6% compared to TY808 and ZD958. Through path analysis, we found that the direct path coefficient of SWS in the VT stage on yield reached 0.999, indicating that soil moisture in the VT stage has the greatest impact on yield, followed by the blister stage (R2). In conclusion, our results suggest that the water consumption of summer maize during the VT stage is the highest, and the soil moisture condition in VT significantly affects the grain yield of summer. Planting DH605 in the North China Plain would harvest the maximum grain yield and water productivity.

The aim of this study was to evaluate the effects of exogenous 2, 4-epibrassinolide (EBR) on the yield of Tartary buckwheat (Fagopyrum tataricum (L.) Gaertn.). A 2-year field experiment was conducted on Tartary buckwheat (cv. Jinqiao 2) with different concentrations (0, 0.1, 0.5, 1.0, and 2.0 mg/L) of EBR and brassinolide (BRZ, inhibitor of brassinolide synthesis). The seed setting rate, agronomic traits, and yield initially increased and then decreased with an increase in the EBR application rate. The seed setting rate, agronomic traits, and yield decreased gradually with an increase in BRZ concentration, and yield was the lowest at 2.0 mg/L. The appropriate application of exogenous EBR could promote the increase of Tartary buckwheat yield. Compared with 0 mg/L (control), the 0.1, 0.5, and 1.0 mg/L treatments increased yield by 13.53, 32.73, and 7.08%, respectively, while the high-concentration treatment (2.0 mg/L) decreased by 4.13%. In conclusion, the appropriate concentration of EBR treatment (0.5 mg/L) delayed the senescence of Tartary buckwheat by increasing its root activity and the activity of antioxidant enzymes in leaves. Simultaneously, it increased the chlorophyll content of Tartary buckwheat leaves, enhanced photosynthesis, increased nonstructural carbohydrate content, and augmented the "source," increasing the seed setting rate and yield of Tartary buckwheat. This concentration is recommended for use in the production of Tartary buckwheat.

The potential of Leersia hexandra grass in phytoremediation and natural attenuation of three groups of bacteria in soil contaminated with crude oil was evaluated for 180 days. The quantities of new shoots, root and aerial biomass were evaluated; changes in antioxidant concentrations in leaf and root caused by abiotic stress; population densities of Azotobacter, Azospirillum and Pseudomonas; and microbial respiration. The experimental data showed oil-induced increases of 315% and 196% in new shoots and root phytomass, respectively, and a 44% decrease in leaf + stem phytomass. The enzymatic defence in the grass leaf was manifested by higher concentrations of hydrogen peroxide, phenylalanine ammonium lyase and total flavonoids; the increases fluctuated from 35% to 52%. The response in the root was positive in catalase (16%), and in ammonium phenylalanine lyase, it increased 275% due to the effect of crude oil. The group of indigenous Azotobacter bacteria were tolerant to crude oil exposure, both in the phytoremediation process and in natural attenuation; the population densities varied from 212 to 438 × 10³ colony-forming units (CFUs); they are greater than 49% to 106% compared to densities in control soil. Azospirillum spp. and Pseudomonas spp. recorded population abiotic stress. The grass activates enzymatic and plant defence, complementing microbial respiration in response to adaptation to crude oil.

Chemical additives can reduce ammonia emissions from ammonium-containing fertilisers. We aimed to investigate the effect of an additive based on carboxylic acid derivatives on ammonia emissions from slurry. In a randomised multi-plot field trial, three slurry treatments with increasing amounts of the additive based on carboxylic acid derivates were tested in comparison to untreated slurry and mineral fertiliser. Ammonia emissions were measured with so-called passive samplers, a method already used in numerous studies. However, problems arose during the evaluation of the collected data, so we examined the methodology used in more detail. The results of the measurements were analysed with regard to their spatial distribution and temporal variation. The results show that the more additives were used, the less ammonia was emitted, up to an emission reduction of 48% at the highest additive application rate. However, the spatial distribution of ammonia emissions reveals a drift of ammonia and, thus, an interaction between the plots. Thus, even in unfertilised plots, ammonia emissions of up to 50% of the treatment with the highest emissions were determined. Furthermore, it was also proven that the different times at which the slurry was applied influenced the level of ammonia emissions. Due to the interaction between the plots and the temporal differences in the application of the slurry, measuring ammonia emissions with passive samplers in multi-plot field trials, as presented in this study, is not suitable to quantify differences between the ammonia emissions from different treatments. Based on these results, recommendations for the use of passive samplers to measure ammonia emissions in field trials are proposed.

The aim of this study was to investigate the physiological characteristics and quality of hypocotyls in the production of selenium-enriched sprouts from peanut seeds soaked in selenium (Se) solution. Peanut seeds were soaked with 0, 2.5, 5.0, 7.5, and 10 μmol/L Na₂SeO₃ for 12 h and then germinated. The results showed that the selenium concentration in peanut shoots increased with increasing levels of selenite soaking, and there existed a crossroads of selenite soaking concentration (5.0 μmol/L) when selenium concentrations in cotyledons and hypocotyls were equal. Below and above this concentration, Se concentrations in shoots were radicle > cotyledon > hypocotyl or cotyledon > radicle > hypocotyl, respectively. In addition, Se significantly promoted the elongation of hypocotyls and radicles, increased shoot biomass, increased the activity of antioxidant enzymes and the concentration of antioxidants in hypocotyls, and decreased malondialdehyde levels. Moreover, Se significantly increased the concentrations of soluble sugars, proteins, free amino acids and resveratrol in hypocotyls. These results indicate that soaking peanut seeds with selenite significantly increased Se concentration, biomass, antioxidant capacity and quality of peanut shoots. This study provides a theoretical basis for the rapid and standardised production of Se-enriched peanut shoots from selenite-soaked seeds.

Carbohydrate digestive enzymes like α-amylase and α-glucosidase can be used to treat and manage diabetes. By inhibiting these enzymes, carbohydrate digestion slowed down, lowering the level of glucose entry into the bloodstream and preventing postprandial hyperglycemia. However, the effectiveness of current antidiabetic agents is limited due to their adverse effects. Therefore, the current study explored natural inhibitors from the methanol extract of rice to combat this issue. Through an integrated approach, four different rice cultivars were analysed and found that red rice methanol extract compounds stigmasterol and 1,2-benzenedicarboxylic acid interacted with α-amylase and α-glucosidase. Additionally, further research on stigmasterol directs the structure-activity relationship studies that aid in managing diabetic conditions.

The effect of drought stress on carbon metabolism in the leaves and roots of bermudagrass was investigated. Plants established in PVC tubes suffered from three water treatments for 10 days. C138 and Tifway (drought-tolerant) were found to have lower relative electrical conductivity and higher water use efficiency than C32 (drought-sensitive) under moderate drought by increasing carotenoid and soluble sugar content and rapidly decreasing leaf starch content. The sucrose synthase activity of leaves and roots, acid invertase and neutral invertase activity of C32 roots substantially decreased under severe drought, resulting in a slow sucrose decomposition rate and significantly lower fructose and glucose contents than C138 and Tifway. The activities of four carbon metabolism enzymes and sucrose content in the leaves were greater than those in the roots, while the fructose and glucose contents were on the contrary, indicating that bermudagrass transported fructose and glucose obtained from sucrose decomposition from leaves to roots under drought to reduce roots damage. The path analysis indicated that leaves neutral invertase activity, and roots soluble sugar content might be the key parameter of carbon metabolism in bermudagrass under drought.

Two-year experiments were conducted on two different soil types during 2021/22 and 2022/23 to study the impact of microbiological preparations, Mycor-FAZOS and Vitality-FAZOS, on the yield and quality elements of winter wheat cultivar Maja (Agrigenetics). The experiment was set up in a split-block design with four repetitions across two soil types, with 12 different variants on each soil type. The base plot area was 40 m2, and the effective plot area was 27 m2. The research factors included: A (soil type): A1 – gley soil; A2 – eutric cambisol; B (disease control): B1 – control; B2 – chemical pesticides; B3 – biopreparates (seed treatment + foliar treatment) and C (nitrogen fertilisation): C1 – based on soil analysis; C2 – 70% of recommended application. Variables included grain yield (t/ha), grain protein content (%), hectoliter weight (kg), and plant height (cm). The highest average grain yield was achieved with variant B3C2, with a 12.50% increase compared to variant B2C1. Differences between these variants included 6.0% for protein content, 5.97% for hectoliter weight, and 7.93% for plant height. It was observed that Mycor-FAZOS seed treatment promoted root development, resulting in healthier, taller, more robust plants with a more uniform growth and darker colour than untreated seed variants (indicating increased photosynthesis index). The protection achieved with Vitality-FAZOS biopreparations (fungicidal microorganisms synthesising growth hormones) ensured healthier wheat plants, leading to higher seed yield and better quality parameters.