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Sugar beet yielding and, thus, the profitability of its cultivation depends on various conditions. These are mainly a dose of nitrogen fertilizing and anti-fungal plant protection. Based on the research carried out in a private-owned farm in Biała commune, Opole province, Poland, it was observed that the most important factors influencing plant development and root yield of sugar beets (var. Kassandra and Cortina) were genetic features of the investigated varieties and fungal control. They increased considerably root yield and sugar content. The doubled nitrogen dose from 90 to 180 kg N/ha enhanced a slight, statistically insignificant, root yield increase and a lower sugar content in beet roots. The three-year research showed that weather conditions during the vegetation period had a decisive effect on sugar beet plants development and fungal infestation. The variety Cortina was characterized with a significantly higher yield and a higher content of treacle forming compounds in the roots. The increase of N rate from 90 to 180 kg N/ha caused a significant increase of average root mass, leaves and dry matter yield and potassium and N-NH₂ in roots, but it also lowered sugar content. In the years with favorable conditions for fungal infestation, the use of fungicides helped to obtain a higher leaf/root ratio, higher root mass, higher root and leaf yield and higher dry matter and sugar yields. However, it did not have an effect on the content of chemical compounds producing treacle in sugar beet roots.

The influence of an interrupted K fertilisation on different K fractions of the soil, yield formation and K uptake by different crops was investigated in a long-term field experiment on Luvisol derived from loess. Irrespective of the previous K fertilisation, the interruption of K fertilisation resulted in a sharp decline of CAL extractable K. K concentration in the saturation extract as well as HCl extractable K were reduced while K fixation capacity increased within 10 years after omitting K. Omitting K fertilisation decreased yields of sugar beet and potatoes while cereals were not affected, although K uptake of all crops reacted to the differentiated K supply to a different extent.

Long-term field experiments conducted under different soil and climate conditions and their databases provide invaluable information and are indispensable means in the study of the productivity and sustainability of the soil management systems. We evaluated the results of the dry matter yields of the main products obtained with four variants of organic and mineral fertilisation in three long-term field experiments established in 1955. The experiments differed in the cultivated crops. The period of evaluation was 12 and 16 years (1985-2000), respectively. The productivity of nine-year crop rotation was lower with the fertilised variants than that with the alternative growing of spring wheat and sugar beets. The dry matter yields on the Nil variants, however, were higher in the crop rotation than in the alternate sugar beet and spring wheat growing, apparently due to the symbiotic nitrogen fixation. The dry matter yields of sugar beet and mainly of spring wheat declined in almost all variants of fertilisation in the alternate sugar beet and spring wheat growing, over the evaluated time period. In spite of the relatively high dry matter production, the declining yields indicated a lower sustainability of the alternate cropping system. Both organic and mineral fertilisation increased the production of the cultivated crops. The differences in the average dry matter yields were statistically significant. Both organic and mineral fertilisation enhanced significantly the N-uptake by the cultivated crops. The effectivity of nitrogen input was the highest with the alternate cropping of sugar beet and spring wheat indicating that it was more demanding for the external N-input and thus less sustainable than nine-year crop rotation.

N fertilization has a substantial influence on sunflower (Helianthus annuus L.) seed yield and quality. It was also well established that high-yielding sunflower hybrids had more N requirement than old cultivars such as open-pollinated ones. However, in Turkey, no sufficient information regarding the response of new developed oilseed sunflower hybrids to nitrogen fertilization under irrigated conditions. Therefore, a 2-year study was conducted to determine the effects of nitrogen application rates on the growth, yield, and yield components of two oilseed sunflower hybrids (AS-508 and Super 25) under irrigated conditions. In this study, all plant parameters were significantly influenced by applied nitrogen fertilizer rates. Yield response to nitrogen rates was positive and linear. Our research data indicated that under irrigated conditions N rate of 120 kg/ha was adequate for sunflower production in this region.

Development of powdery mildew and leaf rust epidemics was examined on three winter wheat cultivars with different predispositions to powdery mildew in three-year experiments. The progress of powdery mildew and leaf rust on the same cultivar was conditioned by its dissimilar susceptibility to the respective disease. Fit temperature played an important role at the beginning of the particular disease and during its progress. Significant differences in the disease severity of powdery mildew and leaf rust were recorded on single leaves. Disease severity of leaf rust was higher on upper leaves while disease severity of powdery mildew was higher on lower leaves.

The Czech University of Agriculture in Prague in cooperation with the Business Exchange in Hradec Králové operates an electronic business system EBS, which enables on-line exchange trade negotiations through the Internet. The system fully takes the advantage of possibilities and technologies of e-business, bringing thus forward new opportunities for making use of the exchange trade with agricultural and food commodities on the EU liberal market. The whole procedure is a part of the Agrarian www portal AGRIS.

The main goal of this work was to calibrate and evaluate the CERES-Barley and CERES-Wheat crop models. The experimental fields used for the model evaluation are situated in three different production regions (maize, sugar beet and potato main growing regions, respectively) with altitudes of 179, 204 and 560 meters above the sea level. Grain yield and date of anthesis together with maturity dates served as reference for the model evaluation. Two evaluation approaches were tested in this study. The first one uses historical data series and it is based on long-term field experiments with capability to reflect interannual weather variability. The second approach uses results of one-year multiple treatment experiment. The model evaluation is then based on a set of treatments differing e.g. in sowing date or an amount of used nitrogen fertilizer. Grain yields simulated by both models are acceptable when compared with experimental results: the coefficient of determination for historical series varied from 0.69 to 0.86 for evaluation of CERES-Barley at the three examined sites and reached values of 0.60 and 0.86 for the CERES-Wheat model at two experimental sites. The lower coefficient of determination of the wheat model was recorded at the locality with the highest altitude and coldest winter conditions. There, also the worst reliability of simulated phenological development was noted. At the second locality where the CERES-Wheat model was tested and at all three localities where CERES-Barley was applied, the simulated duration of vegetation period and anthesis dates were relatively accurate and yielded strong statistical correlation. The one-year multiple treatment experiment proved to be useful to determine the models sensitivity to differences in crop management. The combination of both approaches seems to be the best solution for evaluation of similar crop models if the detail long term experimental data are not available.

The influence of tillage (conventional and non-tillage) on selected soil microbial properties was studied. The experiment focused on tillage that was launched in Prague-Ruzyne in 1997. The experiment included the following variants: conventional tillage, protective tillage (without post harvest residues), protective tillage (with residue biomass - mulch), protective tillage (covered by pea crop residues for winter wheat, with fore crop). In the field experiment there were tested: enzymatic activities (activity of urease, dehydrogenase, arylsulfatase and invertase) and microbial biomass. The microbial biomass and soil microbial processes were affected by tillage. Our results show the positive influence of protection tillage on selected biological parameters, especially in variants where soil was supplied with organic matter.

In the experiment conducted in the greenhouse, the different doses of UV-B radiation applied to the two species Avena fatua and Setaria viridis induced changes in leaf and plant morphology. It was a decrease of plant height, fresh mass of leaves, shoots and roots as well as leaf area. Besides, it caused the leaf curling in both of the species. The significant differences between Avena fatua and Setaria viridis in the studied traits were mainly due to the tillering ability of the species. The content of chlorophyll varied considerably. The average values of leaf greenness (SPAD units) for oats were about 43 while for green foxtail 32, respectively. U-VB did not reduce leaf weight ratio, shoot dry matter, shoot to root ratio and leaf area ratio.

In small-plot field trials conducted in 2000-2002 we studied the effect of fungicides on the yield and selected technological parameters of grain of malt barley and on malt. For the treatment we used Cerelux (active ingredients: fenpropimorph, flusilazole), Amistar (azoxystrobin) and Caramba (metconazole) applied in two stages of stand development (DC 47 and 55). Compared to the untreated control plants the yield grains ranged between 6.9 and 16.5% after the application of fungicides. The application of fungicides increased the proportion of full grains and had a favourable effect on the chemical composition of grain. After the treatment with azoxystrobin the content of N-substances decreased from 11.43 to 11.07% as compared to the controls. The highest starch content of grain was observed after the application of the active ingredient metconazole; the average content ranged between 64.44 and 64.62%. Compared to the controls the highest relative yield of malt starch (124.2-125.2%) was achieved after the application of azoxystrobin or a combination of azoxystrobin and metconazole in the DC 47 stage. The highest average attainable degree of fermentation was 78.57% and was discovered in the untreated control.

An interrelationship between introduced and indigenous rhizobia focused on their competitiveness in nodulation was evaluated in a soil pot experiment. Clover seeds were inoculated by six different gradual concentrations of inoculum of two effective Rhizobium leguminosarum bv. trifolii isolates (inoculation strains 1/2 and 14/2). At the beginning of flowering, clover plants were removed from the pots, and nodules from each pot representing different degrees of inoculum level were taken for reisolate cultivation. The PCR technique was used for the identification of rhizobial reisolates, random amplified polymorphic DNA product patterns were acquired and analysed. Nodule occupation by inoculation strains or indigenous Rhizobium leguminosarum bv. trifolii strains was assessed by comparing the number of nodules formed by inoculation or indigenous strains and inoculation strains competitiveness was calculated. Nodule occupancy by the inoculation strain 1/2 increased sharply with the increase in inoculum dose, whereas in inoculation strain 14/2 more nodules were formed gradually starting from low inoculum level. Competitiveness of inoculation strain 1/2 was calculated as low and was documented by an absence of nodule occupancy in four inoculation levels. On the other hand, competitiveness of the inoculation strain 14/2 was considerably higher, and even in low inoculum dose this strain was more competitive than native rhizobia. Although both the inoculation strains 1/2 and 14/2 were found highly efficient in nitrogen fixation, only the strain 14/2 was able to manifest this characteristic due to the higher competitiveness when applied in lower doses.

As, Cd, Pb and Zn accumulation in the aboveground biomass of seven clones of Salix spp. and changes in element uptake by plants after element addition to soil were studied in a pot experiment. Unpolluted Chernozem (Suchdol) as a control and soils with addition of As (100 mg/kg), Cd (40 mg/kg) and either Pb (2000 mg/kg) (Suchdol-Pb) or Zn (2000 mg/kg) (Suchdol-Zn) were used for the experiment. Significant differences were found in the accumulation of elements between willow clones and also between different element additions to the soil. Although As and Cd uptake slightly increased in Suchdol-Zn soil compared to Suchdol-Pb soil, the element removal from soil was significantly higher in Suchdol-Pb soil due to a significant reduction of aboveground biomass yield in Suchdol-Zn soil caused by Zn phytotoxicity. The yield reduction decreased the uptake of plant-available elements by biomass, thus higher plant-available portions of As and Cd were found in Suchdol-Zn soil. Element removal from soil was more dependent on element contents in willow tissues in Suchdol-Pb soil than in Suchdol-Zn soil, where willow plants exhibited physiological symptoms of phytotoxicity.

Non-symbiotic nitrogen fixation (potential nitrogenase activity - PNA) of soil samples originating from different plots of long-term field experiments (selected variants: Nil, NPK [mineral fertilisation: 64.6-100 kg N/ha/year], FYM [farmyard manure], and FYM + NPK from three blocks III, IV and B with different crop rotation) was determined in laboratory experiments. The symbiotic nitrogen fixation (total nitrogenase activity - TNA) of the same soil samples was evaluated in hydroponic experiments with pea (2001, 2002) and lucerne (2001) in which the soil samples were used as a natural inoculum. The high values of PNA were found in the variants fertilised with FYM in all three blocks and all experiments. Simultaneously, the variants fertilised with mineral NPK reached low values of PNA. The farmyard manuring enhanced the number of free-living bacteria Azotobacter spp. that were identified in all soil samples. In the hydroponic experiments with pea, the highest nonsignificant values of TNA were found in variants B 284 (FYM + NPK) and III 254 (FYM + NPK) in 2001, and B 214 (FYM) and III 214 (FYM) in 2002. Plants inoculated with soil from these variants formed also high amounts of nodules (significant differences in block IV in 2001) and plant biomass. In the experiments with lucerne, the nonsignificantly highest TNA values were found in variant III 154 (NPK). Variants from block III (214, 254) and IV (114 and 154) showed the nonsignificantly lowest TNA values. The rhizobia that effectuate symbiosis with pea were more active in the soil samples in 2001 than those forming nodules on lucerne.

A calibration equation for NIRSystems 6500 instrument was derived at VSTE Jevíčko using the measurement of broad collection of Czech samples of winter rape, allowing sufficiently accurate prediction of content of dry matter (DM), crude protein (XP), crude fat (XL), glucosinolates (GSL), oleic and linoleic acids in an extremely short time. The prediction accuracy was verified on a validation file (n = 60). The coefficients of determinance (R2) were 0.83 for XP, 0.71 for XL, and 0.84 for GSL. The prediction accuracy according to the VSTE equation was compared to the prediction accuracy according to the VDLUFA calibration equation (Kassel, FRG) used in EU near infrared spectroscopy network. It was stated that the former was not distinctly worse. Non-destructive NIR-analysis of the whole seed also allows sowing selected seeds in the year of harvest and thus accelerates the breeding cycle.

The distribution of cadmium, lead and zinc in exchangeable, organic, and 2M HNO3-extractable fractions as well as the effect of heavy metal concentrations on soil microflora was investigated. Six sampling transects were chosen in theLitavkaRiveralluvium in 1999-2001. Concentrations of all metals increased with decreasing distance from the source of contamination. The concentrations of Cd and Zn in exchangeable fraction were higher than in organically bound fraction, a reverse trend was found in Pb speciation. All measured parameters of soil microbial activity were affected by heavy metal concentrations. The decrease in CFU was most significant in the case of oligotrophic bacteria and spore-forming bacteria. Significant inhibition of C-biomass occurred in soils highly contaminated by heavy metals. The Cbiomass:Cox ratio decreased with increasing soil pollution. Generally, the values of enzymatic activities were highest in the soil above the source of contamination and they were decreased as approaching the source of contamination. Our results demonstrate that several parameters of microbial activity could be used as good indicators of increasing concentrations of Cd, Pb, and Zn in soil.

Results of scoring the resistance of 35 selected spring barley varieties to powdery mildew, exhibiting high powdery mildew severity, in 307 variety trials of the Central Institute for Supervising and Testing in Agriculture were analysed. The varieties can be divided into two groups: the varieties that could not induce any changes in the pathogen population (the varieties with no effective resistance gene and varieties carrying gene mlo) and the varieties possessing major resistance genes [a total of 12 Ml-genes: a1, a3, a6, a7, a9, a12, a13, at, k1, La, g and (Kr)] to which the pathogen population adapted in 1971-2000. The time slope of decreasing resistance of varieties is described. The importance of individual evolutionary forces (mutations, migration, direct selection, indirect selection and recombinations) for the erosion of efficiency of respective major resistance genes and the effects of pathogen adaptation on population complexity and diversity are discussed.

The Pb, Zn and Cd phytoextraction potential of 14 different plants was assessed in a chelate induced phytoextraction experiment. In the used soil heavy metals mainly reside in carbonate, organic matter, and residual soil fractions. The addition of a chelate, 5 mmol/kg ethylenediamine-tetracetic acid (EDTA), increased the proportion of phytoavailable Pb, Zn and Cd in the soil (dissolved in soil solution and exchangeable from soil colloids), and also their uptake by tested plants up to 48 times (Sinapis alba), 4.6 times (Raphanus sativus oleiformis), and 3.3 times (Amaranthus spp.), respectively, compared to the control. The biodegradable chelate ethylenediamine-disuccinic acid (EDDS) was generally less effective (tested on a selection of 4 plant species), except for Cannabis sativa. In a treatment with 10 mmol/kg EDDS, Pb, Zn and Cd concentrations of 1053 ± 125, 211 ± 16 and 5.4 ± 0.8 mg/kg, respectively, were measured in the biomass of Cannabis sativa and were 105, 2.3 and 31.7 times higher, respectively, than in the control treatment. The calculated Pb phytoextraction potential of Cannabis sativa amounted to 26.3 kg/ha.

A direct and secondary effect of liming and organic fertilization on the cadmium content in soil and in vegetables was examined. Celeriac (Apium graveolens L. var. rapaceum) was cultivated in the first year after lime application and organic fertilization whereas leek (Allium ampeloprasum ssp. porrum J. Gay) was grown in the next year. Soil was limed with a calcium carbonate fertilizer at the rate of 2.0 t CaO/ha. The following were applied as organic fertilizers: farmyard manure (60 t/ha), rye straw (4 t/ha), rye and winter vetch, both the plants cultivated as winter catch crops for green manure. Liming significantly reduced the available cadmium content in the soil in the first and second year after its application. Also, it significantly reduced the cadmium content in celeriac and leek compared with no-liming cultivation. Organic fertilization, especially farmyard manure and straw, significantly reduced the soil available cadmium content as compared to the untreated control (without organic fertilization). The aforementioned factor significantly reduced the cadmium content in the examined vegetables. Cadmium lowest content in celeriac leaves and leek was recorded after the application of farmyard manure. A combined application of liming and organic fertilization in the form of farmyard manure most beneficially influenced celeriac leaves with respect to their cadmium content.

A morphometric study of Amaranthus cruentus, A. retroflexus and their hybrid, A. × turicensis based on 75 plant samples (750 inflorescences), collected throughout the Olomouc-Holice area (Czech Republic), is presented. Using multivariete methods (including cluster analysis and canonical discriminant analysis), the existence of three groupings of plants was proven. The hybrid exhibited intermediate values of the width and length of female tepals, length of awl-shaped bracts, and seed size when compared with parental species. A germination experiment showed that dark seeds of A. × turicensis as well as dark seeds of A. retroflexus germinate scarcely and independently on the day length while light seeds of A. cruentus germinate promptly and markedly better under a short day regime. The chromosome analysis showed that A. retroflexus, A. cruentus, and A. × turicensis have the same chromosome number 34.

Two methods were developed and tested for the measurement of the mowing machine material feed rate (based on the conditioner power input measured by a torque-meter, and/or on the material change in momentum measured by a curved impact plate). The measurements carried out in the year 2001 proved that a very good linear relationship existed between the conditioner power input, output frequency of the apparatus measuring the impact force by means of the impact plate, and the material feed rate through the mowing machine. The calculated R-Squared values were about 0.95. In the year 2002, the impacts of material and condition (parameters) changes on the mowing machine material feed rate measurement accuracy were measured under laboratory conditions. It was evident from the statistical evaluation that changing crop variety, crop maturity, and intensity of conditioning can have a statistically significant influence on the measurement based on the torque-meter. As concerns the impact plate measurement, it was found that it is not possible on the basis of our measurements to determine statistically the influence of the factors tested.

Twelve carbonaceous materials were investigated for adsorption of Cd, Cu and Ag from water solutions. Before the adsorption experiments the chemical and structural characterization of all materials were made. The batch adsorption experiment was used. The kinetic of the adsorption process was very fast for the first five hours but very slow for approximately the next 65 hours. Nevertheless the maximum amount of metal removed was achieved during the first stage of about five hours. Biological materials (milled wood, bark, cork) exhibited a very low affinity for adsorption of metals. The best results were obtained for materials in an intermediate degree of carbonisation with a high content of oxygen rich functional groups. Although the highly carbonised materials exhibited low ability to adsorb copper or cadmium, their capacity to bind silver was very high.

In 2001-2004, the influence of various ways of farming unmanaged grasslands on the mineral nitrogen content in lysimetric waters was monitored. Field trials with 11 variants were performed 420 m above sea level, at depths of 0.2 and 0.4 m, 4-5 times annually. The average amount of N_min released per ha per year in lysimetric waters ranged from 0.84 to 5.12 kg. The upper layer to a depth of 0.2 m had a higher content. The conclusive difference, in comparison with properly farmed control, was with the black fallow and in the variant where fallow followed mulching. With the exception of this method of farming the pratotechnics of unmanaged grasslands - mulching did not increase the load of rhisosphere, either by the overall mineral nitrogen, or by the concentration of N-NO₃^-. Even by using fallow for a four-year period, a conclusive increase in the content of N_min was not observed.

The effect of phenylurea with reported cytokinin-like activities on seed germination, seedling growth, activities of antioxidant enzymes, polyphenol, peroxidase, indoleacetic acid oxidase, and total phenolic compounds, flavonoids was investigated in stressed barley seedlings. The application of phenylurea decreases the activity of peroxidase, indoleacetic acid oxidase and increases the activity of polyphenol oxidase with decrease in total phenolic compounds and flavonoids and consequent increase in growth rate. Saline (NaCl) stress in barley seedlings causes an increase in total phenolic compounds, flavonoids and enhancement of peroxidase and indoleacetic acid oxidase activities and consequent decrease in growth rate. The adverse effect of salt stress on germination, antioxidant enzymes, phenolic compounds, flavonoids can partially be rectified by phenylurea.

Root growth inhibition and loss of cell viability in barley root cells were induced by Al during germination of barley caryopses on filter paper moistened with 2mM AlCl3. The inhibition of root growth as well as loss of cell viability started on the third day of germination. This time was also needed for induction of Al-induced changes in the composition of cell wall proteins. The accumulation of three salt-extractable polypeptides with relative molecular mass 14, 27, and 29 kDa started 72 h after the beginning of germination of barley caryopses on Al containing filter paper. However, the inhibition of developmentally regulated deposition of three (18, 23 and 28 kDa) salt-extractable CW polypeptides was observed at the same time. The pattern of detergent-extractable CW proteins was not influenced by Al regardless of the duration of Al treatment.

Effects of increasing doses of the herbicidal preparation Sencor 70 WP (metribuzin a.i.) on selected potato varieties were studied in a field and pot experiments using very rapid fluorescence induction (vrFI). Tuber yield, and in pot experiments a tuber number and characteristics of the aboveground biomass were assessed. The curve of rapid induction goes through the O-J-I-P phases. The parameters Fo, Fv/Fp and rFj were measured. Based on changes in the vrFI parameters in comparison with controls, varieties were ranked according to sensitivity to metribuzin in field experiments. Keřkovské rohlíčky and Ukama were the most sensitive varieties and Impala the least sensitive variety. In pot experiments in the growth chamber, significant decrease in tuber weight vs controls was found in sensitive varieties Keřkovské rohlíčky (in 1998, 0.75 kg.ha^-1 Sencor 70 WP decreased tuber weight by 28% and 1.5 kg.ha^-1 by 89%) and Ukama (in 1997, at 1.5 kg.ha^-1 by 35%). Under stable conditions in the growth chamber, there were lower differences in the rFj parameter in these varieties in comparison with controls than in resistant ones. Based on this finding it can be assumed that the sensitivity of varieties can be determined according to rFj changes under stable ambient conditions.

Numerous agricultural and associated ecological effects such as mineral nitrogen fertilising influence the yield of hop (Humulus lupulus L.) cones and its quality. Using a wide spacing of plants (in our case 2.6 × 0.8 m) we want to answer a hypothetical question about an appropriate number of test plants per plot vs. experimental plot area. The aim of this study was to compare the effect of different rates of mineral nitrogen, fertiliser combinations and their nitrogen split application on hop yield evaluated from different plot areas (micro trial: 30 plants per plot; macro trial: 320 plants per plot). Hop yield varied significantly between treatments, plot areas, years and interactions (year × treatment, plot area × treatment) (all at P £ 0.01). Cone yield in a micro trial was higher in all treatments in comparison with yield in a macro trial. In spite of common intensive fertilisation the appropriate fertilising combination and mineral N rate can influence the yield. Target nitrogen rate of160 kg mineral N/ha (at the level from 40.0 to62.5 kg nitrate N/ha in soil depth to0.3 m) and cheaper combination of calcium-ammonium nitrate (50 kg N/ha) at the beginning of vegetation plus urea (110 kg N/ha) for top dressing can be recommended. On plot areas of each size and each year all treatments showed similar trends of fertilising effect on yield. In spite of higher yield in the micro trial and lower coefficient of variation in comparison with the macro trial, the results proved that a risk of incorrect yield analysing in macro trials is very low for field experiments.

A five-year study was conducted to study the correlations between chemical analyses and sensory properties of wine during bottle aging. Chardonnay grapes were harvested as a normal and late harvest. After separate vinification, bottles were put in an underground cellar at12°Cand 75% of humidity. Chemical and sensory analyses were carried out after bottling (0), after 12, 24 and 36 months of bottle aging. The results of chemical and sensory evaluation show a strong correlation between a young wine bouquet and decrease in the concentration of isoamyl and 2-phenethyl acetates, and between an increase in diethyl succinate and bottle bouquet.

Dependently on planting conditions caraway fruits contain 1-9% of essential oils consisting of about 30 compounds. Carvone and limonene account for the main portion, about 95%. To evaluate the quality of various registered caraway (Carum carvi L.) cultivars (Kepron, Prochan and Rekord) planted during 1998-2000, regarding the effect of sample grinding and preparation, plant treatment and time of harvest, the amounts of essential oil and the carvone/limonene ratio were determined. Both whole and ground caraway seeds were extracted. As obvious from the results, SFE is not suitable for the determination of essential oils in whole seeds since the results are lower in comparison with those of ground caraway. The way of grinding was also examined. Of the three mills used, a splintery mill VIPO seemed to be the most suitable. Further, the amount of essential oil was studied in caraway gathered at the beginning of maturation (sample A) and at full ripeness (sample B) of caraway seeds. It was found that the samples gathered at full ripeness (samples B) had more essential oil. It was also concluded that the use of the regulator Roundup Bioaktiv during caraway maturation to unite the ripening of achenes in the main umbel and the first-order umbels, and the use of fungicides (Alert S and Prelude 10) affected positively the amount of essential oil in caraway. Possibilities of SFE application for the essential oil determination in small samples gathered during breeding were investigated. The results were used as a one of the criteria during breeding. Classical way of the essential oil determination does not allow this option. An alternative method for the isolation and determination of essential oils - supercritical fluid extraction (SFE) - was investigated in this study.

The observations were conducted on chernozem soil in a sugar-beet production region in 1971-2002. Six variants of organic amendment were examined: l - straw harvest, 2 - straw harvest + green manuring, 3 - straw incorporation, 4 - straw incorporation + green manuring, 5 - straw burning, 6 - straw burning till 1977 + intercrop, incorporation of farmyard manure at 10 t/ha since 1978. The effect of organic amendment on winter wheat continuous cropping was statistically significant. On average of the whole period (32 years), the highest yields were produced in variants with straw burning (var. 5) - 6.04 t/ha and with green manuring (var. 2) - 6.03 t/ha, and lower yields in variants with straw incorporation into soil (var. 3) - 5.65 t/ha, (var. 4) - 5.67 t/ha. The smallest differences between variants were found in the first decade of the experiment. Over time (in the second and third decades), the differences increased and positive effects of green manuring and straw burning and adverse effects of straw incorporation into soil increased. The yield level of winter wheat continuous cropping was high under the given conditions. The yield for the whole period of the experiment averaged 5.85 t/ha, the yields 5.19, 6.54 and 5.76 t/ha were obtained in the first, second and third decade, respectively.