Plant Soil Environ., 2002, 48(1):15-19 | DOI: 10.17221/4199-PSE

Variation in HMW glutenin subunits of different species of wheat

Z. Gálová, I. Michalík, H. Knoblochová, E. Gregová
1 Slovak Agricultural University in Nitra, Slovak Republic
2 Research Institute of Plant Production, Piešťany, Slovak Republic

Method ISTA SDS-PAGE was used for separation, detection and evaluation of high molecular weight glutenin subunits (HMW) in the different wheat species. The relation has been studied between the HMW glutenin subunit alleles and the bread-making quality of 25 world wheat cultivars and 21 regional varieties common wheat varieties (Triticum aestivum L.), 17 winter spelt wheat (Triticum spelta L.), 3 durum wheat cultivars (Triticum durum DESF.), 9 cultivars of Triticum turgidum L. and 5 cultivars of Triticum polonicum L. The highest frequency of occurrence of HMW glutenin subunits 2*, 13 + 16 and 5 + 10 were found in world wheat cultivars. In Slovak wheat varieties were analysed subunits 0, 7 + 9 and 5 + 10, 2 + 12. The HMW subunits 0, 7 + 8 with Glu-score 4 were determined in Triticum durum DESF. Three electrophoretical profile groups of different HMW glutenin subunits were found in Triticum turgidum L. and Triticum polonicum L. and six electrophoretical profile groups were determined in Triticum spelta L. The verified correlations between bread-making quality and specific HMW subunits of glutenin can be utilised by wheat breeders using SDS-PAGE of proteins as a screening test for the prediction of bread-making quality of wheat.

Keywords: Triticum aestivum L.; Triticum durum DESF.; Triticum turgidum L.; Triticum polonicum L.; Triticum spelta L.; HMW glutenin subunits; electrophoresis SDS-PAGE; SDS sedimentation test

Published: January 31, 2002  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Gálová Z, Michalík I, Knoblochová H, Gregová E. Variation in HMW glutenin subunits of different species of wheat. Plant Soil Environ. 2002;48(1):15-19. doi: 10.17221/4199-PSE.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Abdel-Aal E.S.M., Salama D.A., Hucl P., Sosulski F.W., Cao W. (1996): Electrophoretic characterization of spring spelt wheat gliadins. J. Agric. Food Chem., 44: 2117–2123. Go to original source...
    2. Campbell K.G. (1997): Spelt: agronomy, genetics and breeding. Pl. Breed. Rev., 15: 187–213. Go to original source...
    3. Èerný J., Šašek A. (1996): Bílkovinné signální geny pšenice obecné. ÚZPI, Praha.
    4. Gálová Z., Smolková H., Michalík I., Gregová E. (1998): Prediction of bread-making quality of wheat grain on the base of electrophoretic spectra of HMW glutenin subunits. Rostl. Výr., 44: 111–116.
    5. Gregová E., Kraic J., Žák I. (1995): Charakterizácia odrôd pšenice pomocou glutenínov. In: Biochemické, molekulárne a morfologické techniky v identifikácii odrôd rastlín. ÚKSÚP, Bratislava: 11–14.
    6. Harsch S., Gunther T., Kling Ch.I., Rozynek B., Hesemann C.U. (1997): Characterization of spelt (Triticum spelta L.) forms by gel electrophoretic analyses of seed storage proteins. Theor. Appl. Genet., 94: 52–60. Go to original source... Go to PubMed...
    7. Kraic J., Gregová E., Tisová V. (1997): Genetic variability at the Glu-1 loci in old and modern wheats (Triticum aestivum L.) cultivated in Slovakia. Genet. Res. Crop Evol., 44: 301–306. Go to original source...
    8. Payne P.I., Lawrence G.J. (1983): Catalogue of allelesfor the complex gene loci, Glu-A1, Glu-B1 and Glu-D1 which code for high-molecular-weight subunits of glutenin in hexaploid wheat. Cereal Res. Communs, 11: 29–35.
    9. Payne P.I., Nightingale M.A., Krattiger A.F., Holt L.M. (1987): The relationship of HMW glutenin subunit composition and the bread-making quality of British-grown wheat varieties. J. Sci. Food Agric., 40: 51–66. Go to original source...
    10. Rodriguez-Quijano M., Vázquez J.F., Carrillo J.M. (1990): Variation of high molecular weight glutenin subunits in Spanish landraces of Triticum aestivum ssp. vulgare and ssp. spelta. J. Genet. Breed. 44: 121–126. Šašek A., Èerný J. (1997): Gliadinové a gluteninové signální geny pšenice špaldy (Triticum spelta L.). Rostl. Výr., 43: 149–151.
    11. Vlasák M. (1995): Možnosti pìstování pšenice špaldy (Triticum spelta L.) v Èeské a Slovenské republice. Genet. Zdroje Rastl., 6: 43–48.
    12. Wrigley C.W. (1992): Identification of cereal varieties by gel electrophoresis of the grain proteins. In: Seed analysis. Springer Verlag, Berlin, Heilderberg: 17–41. Go to original source...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content