Plant Soil Environ., 2002, 48(2):58-62 | DOI: 10.17221/4360-PSE

The influence of diesel oil contamination on soil enzymes activity

J. Wyszkowska, J. Kucharski, E. Wa³dowska
University of Warmia and Mazury in Olsztyn, Poland

A pot experiment was conducted in order to examine the influence of soil contamination with diesel oil at 0.0, 2.4, 4.8 and 7.2 ml/kg on the activity of dehydrogenases, urease, acid phosphatase and alkaline phosphatase. The results indicated that diesel oil contamination of soil strongly inhibited the activity of dehydrogenases and soil urease, but had only a slight effect on the activity of acid phosphatase and alkaline phosphatase. The negative influence of diesel oil on the activity of dehydrogenases and urease was attenuated by soil inoculation with Streptomyces intermedius spores. The potential biochemical index of soil fertility computed from the soil enzymatic activity and carbon content was negatively correlated with diesel oil contamination and positively correlated with crop yield. Biochemical properties of soil were improved by oat cultivation.

Keywords: diesel oil; soil enzymes activity

Published: February 28, 2002  Show citation

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Wyszkowska J, Kucharski J, Wa³dowska E. The influence of diesel oil contamination on soil enzymes activity. Plant Soil Environ. 2002;48(2):58-62. doi: 10.17221/4360-PSE.
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References

  1. Casida L.E, Klein J.D., Santoro D. (1964): Soil dehydrogenases activity. Soil Sci., 98: 371–374. Go to original source...
  2. Galas E., Kwapisz E., Torbisz-Szymañska L., Krystynowicz A., Antczak T., Oryñska A. (1997): Charakterystyka wybranych szczepów bakterii degraduj<sup>1</sup>cych wêglowodory ropy naftowej. Biotechnol., 36: 145–157.
  3. Gorin G., Chine Chang Ch. (1966): A new method of assay the specific enzymic activity. IV. Urease. Analyt. Biochem., 17: 49–58. Go to original source... Go to PubMed...
  4. Gray M.R., Banerjee D.K., Fedorak P.M., Hashimoto A., Masliyah J.H., Pickard M.A. (1994): Biological remediation of anthracene-contaminated soil in rotating bioreactors. Appl. Microbiol. Biotechnol., 40: 933–940. Go to original source...
  5. Kobus J. (1995): Biologiczne procesy a kszta<sup>3</sup>towanie ¿yznoœci gleby. Zesz. Probl. Post. Nauk Rol., 421a: 209– 219.
  6. Kucharski J. (1997): Relacje miêdzy aktywnoœci<sup>1</sup> enzymów a ¿yznoœci<sup>1</sup> gleby. In: Barabasz W. (ed.): Drobnoustroje a ¿ycie gleby. AR, Kraków: 327–341.
  7. Kucharski J., Wyszkowska J. (2001): Microbiological properties of soil contaminated with diesel oil. Acta Agroph., 51: 113–120. Go to original source...
  8. Leahy J.G., Colwell R.R. (1990): Microbial degradation of hydrocarbons in environment. Microbiol. Rev., 54: 305–315. Go to original source... Go to PubMed...
  9. Ma<sup>3</sup>achowska-Jutsz A., Mrozowska J., Kozielska M., Miksch K. (1997): Aktywnoœae enzymatyczna w glebie ska¿onej zwi<sup>1</sup>zkami ropopochodnymi w procesie jej detoksykacji. Biotechnol., 36: 79–91.
  10. Olson J.J., Milis G.L., Herbert B.E., Morris P.J. (1999): Biodegradation rates of separated diesel components. Environ. Toxicol. Chem., 18: 2448–2453. Go to original source...
  11. Opic J. (1996): Siew bezpoœredni a w<sup>3</sup>aœciwoœci chemiczne i aktywnoœae biologiczna gleby. Post. Nauk Roln., 6: 25–32.
  12. Riis V., Miethe D., Babel W. (1995): Degradation of refinery products and oils from polluted sites by the autochthonous microorganisms of contaminated and pristine soils. Microbiol. Res., 150: 323–330. Go to original source... Go to PubMed...
  13. StatSoft, Inc. 2000. STATISTICA for Windows [Computer program manual]. Tulsa, OK: StatSoft, Inc. 2300 East 14th Street, Tulsa, OK 74104, http://www.statsoft.com.
  14. Tabatabai M.A., Bremner J.M. (1969): Use of p-nitrophenyl phosphate for assay of soil phosphatase activity. Soil Biol. Biochem., 1: 307–310. Go to original source...
  15. Wiesel I., Wubker S.M., Rehm H.J. (1993): Degradation of polycyclic aromatic hydrocarbons by an immobilized mixed bacterial culture. Appl. Microbiol. Biotechnol., 39: 110–116. Go to original source...
  16. Wyszkowska J., Kucharski J. (2000): Biochemical properties of soil contaminated by petrol. Polish J. Envir. St., 9: 479–485.
  17. Wyszkowska J., Kucharski J., Wa<sup>3</sup>dowska E. (2002): The influence of diesel oil contamination on soil microorganisms and oat growth. Rostl. Vżr., 48: 51–57. Go to original source...
  18. Zieñko J. (1996): Substancje ropopochodne w œrodowisku przyrodniczym. Cz. I. Kryteria i ocena stopnia zanieczyszczenia. Ekol. Techn., 4: 18–23.

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