Plant Soil Environ., 2003, 49(3):99-105 | DOI: 10.17221/4097-PSE

Evaluation of protein fractions as biochemical markers for identification of spelt wheat cultivars (Triticum spelta L.)

V. Dvořáček, V. Čurn
1 Research Institute of Crop Production, Prague-Ruzyně, Czech Republic
2 Faculty of Agronomy, University of South Bohemia in České Budějovice, Czech Republic

Four protein fractions: 1 - albumins and globulins, 2 - gliadins, 3 - glutenins (extracted in NaOH), 4 - glutenins (extracted in SDS) separated by SDS-PAGE were used as biochemical markers for evaluation of polymorphism level in three spelt wheat cultivars - Hercule, Altgold and Rouquin, three new-breeders' spelt lines - H92.27, H92.28 and M92.20 (originated from hybridisation between spelt and common wheat) and reference common wheat cultivar Brea. Electrophoretic phenotypes and zymograms were evaluated by means of digital image analysis and Nei and Li coefficient of similarity was used to evaluate the relation of analysed genotypes. Entire evaluation of all four-marker systems showed differences between common wheat cultivarBreaand spelt cultivars and spelt breeders' lines. Also significant differences between old spelt cultivars (Hercule, Altgold and Rouquin) and new spelt breeders' lines were found. The reality of the mutual passing of protein fractions (gliadins and glutenins), based on Osborne extraction was confirmed. In this sense it is necessary to see both fractions as dynamic overlapping structures.

Keywords: spelt wheat; biochemical markers; seed proteins; electrophoresis; protein fractions

Published: March 31, 2003  Show citation

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Dvořáček V, Čurn V. Evaluation of protein fractions as biochemical markers for identification of spelt wheat cultivars (Triticum spelta L.). Plant Soil Environ. 2003;49(3):99-105. doi: 10.17221/4097-PSE.
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    References

    1. Abdel-Aal E.S.M., Salama D.A., Hucl P., Sosulski F.W., Cao W. (1996): Electrophoretic characterization of spring spelt wheat gliadins. J. Agric. Food Chem., 44: 2117-2123. Go to original source...
    2. Brdička R. (1981): Genetické polymorfismy enzymů a bílkovin. Avicenum, Praha.
    3. Byers M., Miflin B.J., Smith S.J. (1983): A quantitative comparison of the extraction of protein fractions from wheat grain by different solvents, and of the polypeptide and amino acid composition of the alcohol-soluble proteins. J. Sci. Food Agric., 30: 447-462. Go to original source... Go to PubMed...
    4. Černý J., Šašek A. (1996): Bílkovinné signální geny pšenice obecné. ÚZPI, Praha: 1-66.
    5. Dvořáček V., Čurn V., Moudrý J. (2001a): Methodological approach to evaluation of biochemical markers in spelt wheat (Triticum spelta L.). Scientia Agric. Bohem., 32: 171-182.
    6. Dvořáček V., Čurn V., Moudrý J. (2001b): Polymorphism of biochemical markers in selected cultivars of spelt wheat (Triticum spelta L.). Rostl. Výr., 47: 77-81.
    7. Dvořáček V., Čurn V., Moudrý J. (2001c): Studies of protein fraction in grain of spelt (Triticum spelta L.) and common wheat (Triticum aestivum L.). Scientia Agric. Bohem., 32: 287-305.
    8. Dvořáček V., Čurn V., Moudrý J. (2002): Evaluation of amino acid content and composition in spelt wheat varieties. Cereal Res. Commun., 30: 187-193. Go to original source...
    9. Field J.M., Shewry P.R., Miflin B.J., March J.F. (1982): The purification and characterization of homologous high-molecular-weight storage proteins from grain of wheat, rye and barley. Theor. Appl. Genet., 62: 329-336. Go to original source... Go to PubMed...
    10. Harsch S., Günter T., Kling Ch.I., Rozynek B. (1997): Characterization of spelt (Triticum spelta L.) forms by gel-electrophoretic analyses of seed storage proteins. I. The gliadins. Theor. Appl. Genet., 94: 52-60. Go to original source... Go to PubMed...
    11. Kim H.R., Bushuk W. (1995): Changes in some physicochemical properties of flour proteins due to partial reduction with dithiotreitol. Cereal Chem., 72: 450-456.
    12. Knoblochová H., Gálová Z. (2000): High molecular weight glutenin subunits variation and relative quantitation in winter spelt wheat cultivars. Rostl. Výr., 46: 255-260.
    13. Kovach L. (1995): Manual MVSP. Pentreath, Anglesey, Wales.
    14. Králová M. et al. (1991): Vybrané metody chemické analýzy půd a rostlin. Academia, Praha: 91-92.
    15. Miflin B.J., Field J.M., Shewry P.R. (1983): Cereal storage proteins and their effect on technological properties. In: Daussant J. et al. (eds.): Seed proteins. Acad. Press, New York: 255-319.
    16. Mosse J., Baudet J. (1963): Conditions of aqueous extraction of water-soluble flour proteins. In: Pomeranz Y. et al. (eds.): Wheat chemistry and technology I. AACC-Minesota: 167-168.
    17. Moudrý J. (1999): Productivity of spelt wheat (Triticum spelta L.) spike. Scientia Agric. Bohem., 30: 15-26.
    18. Moudrý J., Dvořáček V. (1999): Chemical composition of grain of different spelt (Triticum spelta L.). Rostl. Výr., 45: 533-538.
    19. Nielsen G. (1985): The use of isozymes as probes to identify and label plant cultivars and cultivars. Curr. Top. Biol. Med. Res., 12: 1-32.
    20. Ovesná J., Nováková I., Kučera L., Svobodová S., Dotlačil L. (2001): Variability of storage proteins in some Czech landraces, modern and obsolete cultivars of wheat (Triticum aestivum L.). Rostl. Výr., 47: 82-90.
    21. Pomeranz Y. et al. (1988): Wheat chemistry and technology I. AACC-Minesota: 159-253.
    22. Radić-Miehle H., Saam C., Hüls R., Kling I.Ch., Heseman C.U. (1997): Characterization of spelt (Triticum spelta L.) forms by gel-electrophoretic analyses of seed storage proteins. II. The glutenins. Theor. Appl. Genet., 94: 882-886. Go to original source...
    23. Radić-Miehle H., Saam C., Hüls R., Kling I.Ch., Heseman C.U. (1998): Characterization of spelt (Triticum spelta L.) forms by gel-electrophoretic analyses of seed storage proteins. III. Comparative analyses of spelt and Central European winter wheat (Triticum aestivum L.) cultivars by SDS-PAGE and acid-PAGE. Theor. Appl. Genet., 97: 1340-1346. Go to original source...
    24. Šašek A., Černý J. (1997): Gliadinové a gluteninové signální geny pšenice špady (Triticum spelta L.). Rostl. Výr., 43: 149-151.
    25. Wrigley C.W. (1992): Identification of cereal varieties by gel electrophoresis of the grain proteins. In: Linskens H.F., Jackson J.F.: Seed analyses. Springer-Verlag, Berlin, Heidelberg: 17-41. Go to original source...

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