Plant Soil Environ., 2003, 49(4):151-157 | DOI: 10.17221/4106-PSE

Activities of amine oxidase, peroxidase and catalase in seedlings of Pisum sativum L. under different light conditions

L. Luhová, A. Lebeda, D. Hedererová, P. Peč
Faculty of Science, Palacký University in Olomouc, Czech Republic

The activities of amine oxidase, peroxidase and catalase were studied in 12 cultivars of field pea (Pisum sativum L.) and one accession of wild pea (Pisum sativum subsp. transcaucasicum). The influence of different light conditions on the enzyme activities was studied in extracts of 8-d-old seedlings. Substantially higher amine oxidase activity was detected in etiolated pea seedlings than in plants growing under controlled light conditions (12h photoperiod). Higher peroxidase and catalase activities indicate more intensive production of toxic hydrogen peroxide evolved by reactions of different type in green plants in comparison with etiolated ones. Significantly lower activity of peroxidases in etiolated plants could be related to a lower degree of lignification. Marked differences in enzyme activities between etiolated field pea and P. sativum subsp. transcaucasicum were observed for all studied enzymes. A very interesting result was the exceptionally low activity of amine oxidase in etiolated plants that was hardly detectable in green plants of Malton cultivar. Two bands with amine oxidase activity were found by the method of native PAGE in extracts of 8-d-old plants. A different relationship of these isoenzymes was detected in field pea and wild pea. Two isoenzymes were present in pea shoots but only one isoenzyme was detected in pea roots. Amine oxidase isoenzymes were studied in the roots and shoots of cv. Smaragd for three weeks. The profile of isoenzymes was opposite in 8- and 36-d-old stems of pea.

Keywords: amine oxidase; peroxidase; catalase; pea; isoenzymes; lignification

Published: April 30, 2003  Show citation

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Luhová L, Lebeda A, Hedererová D, Peč P. Activities of amine oxidase, peroxidase and catalase in seedlings of Pisum sativum L. under different light conditions. Plant Soil Environ. 2003;49(4):151-157. doi: 10.17221/4106-PSE.
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    References

    1. Angelini R., Bragaloni M., Federico R., Infantino A., Portapuglia A. (1993): Involvement of polyamines, diamine oxidase and peroxidase in resistance of chickpea to Ascochyta rabiei. J. Plant Physiol., 142: 704–709. Go to original source...
    2. Angelini R., Manes F., Federico R. (1990): Spatial and functional correlation between diamine-oxidase and peroxidase activities and their dependence upon de-etiolation and wounding in chick-pea stems. Planta, 182: 89–96. Go to original source... Go to PubMed...
    3. Bachrach U. (1985): Copper amine oxidases and amines as regulators of cellular processes. In: Mondovi B. (ed.): Structure and functions of amine oxidases. CRC Press, Boca Raton, Florida: 5–20. Go to original source...
    4. Bate N.J., Orr J., Ni W.T., Meromi A., Nadlerhassar T., Doerner P.W., Dixon R.A., Lamb C.J., Elkind Y. (1994): Quantitative relationship between phenylalanine ammonia-lyase levels and phenylpropanoid accumulation in transgenic tobacco identifies a rate-determining step in natural product synthesis. Proc. Nat. Acad. Sci., USA, 91: 7608–7612. Go to original source... Go to PubMed...
    5. Bradford M.M. (1976): A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72: 248–254. Go to original source... Go to PubMed...
    6. Chance B., Sies H., Boveris A. (1979): Hydroperoxide metabolism in mammalian organs. Physiol. Rev., 59: 527–605. Go to original source... Go to PubMed...
    7. Cogoni A., Farci R., Medda R., Rinaldi A., Floris G. (1989): Amine oxidase from Lathyrus cicera and Phaseolus vulgaris: purification and properties. Prep. Biochem., 19: 95–112. Go to original source... Go to PubMed...
    8. Federico R., Angelini R. (1986): Occurrence of diamine oxidase in the appoplast of pea epicotyls. Planta, 167: 300–302. Go to original source... Go to PubMed...
    9. Federico R., Angelini R. (1988): Distribution of polyamines and their related catabolic enzyme in etiolated and lightgrown Leguminosae seedlings. Planta, 173: 317–321. Go to original source... Go to PubMed...
    10. Frébort I., Haviger A., Peè P. (1989): Employment of guaiacol for the determination of activities of enzymes generating hydrogen peroxide and for the determination of glucose in blood and urine. Biologia (Bratislava), 44: 729–737.
    11. Góth L. (1991): A simple method for determination of serum catalase and revision of reference range. Clin. Chim. Acta, 196: 143–152. Go to original source... Go to PubMed...
    12. Gould S.J., Keller G.A., Subramanl S. (1988): Identification of peroxisomal targeting signal located at the carboxy terminus of four peroxisomal proteins. J. Cell Biol., 107: 897–905. Go to original source... Go to PubMed...
    13. Houen G., Leonardsen L. (1992): A specific peroxidase-coupled activity stain for diamine oxidases. Anal. Biochem., 204: 296–299. Go to original source... Go to PubMed...
    14. Jackson P., Ricardo C.P.P. (1994): An examination of the peroxidases from Lupinus albus L. hypocotyls. Planta, 194: 311–317. Go to original source...
    15. Joshi B.H., Prakash V. (1982): Naturally occurring pea diamine oxidase inhibitors obtained from Sorghum vulgare during germination. Experientia, 38: 315–319. Go to original source...
    16. Lebeda A., Janèová D., Luhová L. (1999): Enzymes in fungal plant pathogenesis. Phyton-Ann. Rei Bot., 39: 51–56.
    17. Lebeda A., Luhová L., Sedláøová M., Janèová D. (2001): The role of enzymes in plant-fungal pathogens interactions. J. Plant Dis. Prot., 108: 89–111.
    18. Liu L., Eriksson K.E.L., Dean J.F.D. (1995): Localization of hydrogen peroxide production in Pisum sativum L. using epi-polarization microscopy to follow cerium perhydroxide deposition. Plant Physiol., 107: 501–506. Go to original source... Go to PubMed...
    19. Low P.S., Merida J.R. (1996): The oxidative burst in plant defense: Function and signal transduction. Physiol. Plantarum, 96: 532–542. Go to original source...
    20. Luhová L., Janèová D., Frébort I., Lebeda A., Šebela M., Køístková E., Peè P. (1999): Amine oxidase, peroxidase, catalase and acid phosphatase activities in powdery mildew infected plants of Cucumis sativus. Phyton-Ann. Rei Bot., 39: 235–241.
    21. Luhová L., Šebela M., Frébort I., Zajoncová L., Faulhammer H.G., Peè P. (1998): Screening of the occurrence of copper amine oxidases in Fabaceae plants. Biol. Plantarum, 41: 241–254. Go to original source...
    22. Maccarrone M., Bladergroen M.R., Finazzi-Agro A. (1996): Amine oxidase distribution in different organs of lentil seedlings. Plant Physiol. Biochem., 34: 473–478.
    23. Maccarrone M., Rossi A., Avigliano L. (1991): Activity and expression of diamine oxidase in lentil seedlings under different growth conditions. Plant Sci., 79: 51. Go to original source...
    24. Macholán L., Mináø J. (1974): The depression of synthesis of pea diamine oxidase due to light and the verification of its participation in growth processes using competitive inhibitors. Biol. Plantarum, 16: 86–93. Go to original source...
    25. Medda R., Padiglia A., Floris G. (1995): Plant copper-amine oxidases. Phytochemistry, 39: 1–9. Go to original source...
    26. Montalbini P., Buonaurio R., Umesh-Kumar N.N. (1995): Peroxidase activity and isoperoxidase pattern in tobacco leaves infected with tobacco necrosis virus and other viruses inducing necrotic and non necrotic alternations. J. Phytopathol., 143: 295–301. Go to original source...
    27. Raina A., Janne J. (1975): Physiology of the natural polyamines putrescine, spermidine and spermine. Med. Biol., 53: 121–147. Go to PubMed...
    28. Rea G., Laurenzi M., Tranquilli E., D’Ovidio R., Federico R., Angelini R. (1998): Developmentally and wound-regulated expression of the gene encoding a cell wall copper amine oxidase in chickpea seedlings. FEBS Lett., 437: 177–182. Go to original source... Go to PubMed...
    29. Samec P., Pošvec Z., Stejskal J., Našinec V., Griga M. (1998): Cultivar identification and relationships in Pisum sativum L. based on RAPD and isoenzymes. Biol. Plantarum, 41: 39–48. Go to original source...
    30. Scandalios J.G. (1994): Regulation and properties of plant catalases. CRC Press, Boca Raton, Florida.
    31. Siegel B.Z. (1993): Plant peroxidases – an organismic perspective. Plant Growth Regul., 12: 303–312. Go to original source...
    32. Sindhu R.K., Desai H.V. (1980): Inhibitor of diamine oxidase in cotyledon of groundnut seedlings. Phytochemistry, 19: 317–318. Go to original source...
    33. Wilson M.B., Nakane P.K. (1978): Immonofluorescence and related staining techniques. Elsevier/North Holland Biomedical Press, Amsterdam.
    34. Wojtaszek P. (1997): Oxidative burst: an early plant response to pathogen infection. Biochem. J., 322: 681–692. Go to original source... Go to PubMed...

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