Plant Soil Environ., 2003, 49(6):255-260 | DOI: 10.17221/4122-PSE

Application of sonication-assisted Agrobacterium-mediated transformation in Chenopodium rubrum L

J.I. Flores Solís, P. Mlejnek, K. Studená, S. Procházka
Mendel University of Agriculture and Forestry in Brno, Czech Republic

Chenopodium rubrum belongs to the plant species in which standard Agrobacterium-mediated transformation procedures remain inefficient. We demonstrate that the employment of sonication-assisted Agrobacterium-mediated transformation (SAAT) effectively enhanced transient expression of GUS gene coding for b-glucuronidase in Chenopodium rubrum. Further the results indicated that the age of seedlings is one of the limiting factors affecting the potency of Agrobacterium tumefaciens infection. Histochemical detection of b-glucuronidase activity revealed that two-days-old seedlings were much more susceptible to infection than ten-days-old ones. According to our results SAAT technology could provide an efficient tool for obtaining stable transformants when applied to two-days-old seedlings of Chenopodium rubrum.

Keywords: Agrobacterium tumefaciens; strain EHA 105; uidA; GUS; b-glucuronidase

Published: June 30, 2003  Show citation

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Flores Solís JI, Mlejnek P, Studená K, Procházka S. Application of sonication-assisted Agrobacterium-mediated transformation in Chenopodium rubrum L. Plant Soil Environ. 2003;49(6):255-260. doi: 10.17221/4122-PSE.
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