Plant Soil Environ., 2003, 49(11):486-491 | DOI: 10.17221/4182-PSE

Suitability of oat-seed storage-protein markers for identification of cultivars in grain and mixed flour samples

V. Dvořáček, V. Čurn, J. Moudrý
1 Research Institute of Crop Production, Prague-Ruzyně, Czech Republic
2 Faculty of Agronomy, University of South Bohemia in České Budějovice, Czech Republic

The objective of this study was an improvement on oat identification procedure for laboratory applications, and the comparison of albumin-globulin and avenin protein patterns in five hulled and naked oat cultivars: Abel (CZ) and Izák (CZ) - naked oats, Auron (CZ), Edmund (D) and Expander (D) - hulled oats. The last object of this study was the authenticity verification of standardly prepared meal samples with various proportions of admixture. It was confirmed that avenins, characterised under SDS-PAGE conditions, are reliable implements for the identification of oat cultivars. It was found that oat grain contains, on the basis of Osborne fractionation, another significant protein fraction - glutelins. The question of the protein fraction analysis that was used for the admixture identification stays still open. In sufficiently different cultivars, the certainty of the admixture detection in meal samples may be high. Nevertheless, in other cases (higher cultivar similarity) it will be necessary to use some other, more sensitive techniques.

Keywords: oat; cultivar identification; protein fraction; electrophoresis; PAGE

Published: November 30, 2003  Show citation

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Dvořáček V, Čurn V, Moudrý J. Suitability of oat-seed storage-protein markers for identification of cultivars in grain and mixed flour samples. Plant Soil Environ. 2003;49(11):486-491. doi: 10.17221/4182-PSE.
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