Plant Soil Environ., 2004, 50(2):59-64 | DOI: 10.17221/3681-PSE

Using DNA markers for characterisation of tomato resistance against root nematoden Meloidogyne incognita

S. Skupinová, P. Vejl, P. Sedlák, M. Bardová, L. Srbek, P. Klápště, M. Zouhar, B. Tesařová
1 Czech University of Agriculture in Prague, Czech Republic
2 Moravoseed Ltd., Mikulov-Mušlov, Czech Republic

CAPS (Cleaved Amplified Polymorphic Sequence) method and standard infection tests were used for the study of tomatoes (Lycopersicon esculentum L.) resistance against the root-knot nematode (Meloidogyne incognita Kofoit and White). The CAPS method was used to determine genotypic constitution of Mi gene in ten Czech and four foreign varieties of tomatoes. Similarly were verified one hundred and twenty individuals of F2 segregated progeny of Nema variety and the same number of F2 progeny of Petopride variety. A comparison of results of infection test with results of molecular-genetic analyse of DNA showed 100% congruence in detection resistant (susceptible respectively) genotypes. Result of 2 test confirmed in the level of mathematical expectation P (0.5, 0.7) congruence between actually obtained and theoretical segregation ratios. The CAPS method can be recommended for fast and precise determination of resistance or susceptibility of tomato plants against the root-knot nematodes. The goal of the method is the possibility of detection of genotypic constitution of Mi gene that can significantly accelerate the process of creation of new resistant varieties.

Keywords: tomato; Lycopersicon esculentum L.; Meloidogyne incognita Kofoit and White; resistance; CAPS; DNA; marker; PCR

Published: February 29, 2004  Show citation

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Skupinová S, Vejl P, Sedlák P, Bardová M, Srbek L, Klápště P, et al.. Using DNA markers for characterisation of tomato resistance against root nematoden Meloidogyne incognita. Plant Soil Environ. 2004;50(2):59-64. doi: 10.17221/3681-PSE.
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    References

    1. Bajaj K.L., Arora Y.K., Mahajan R. (1983): Biochemical differences in tomato cultivars resistant and susceptible to Meloidogyne incognita. Rev. Nematol., 6: 143-145.
    2. Beek J.G. (1997): Interaction between root-knot nematodes and Solanum spp. [Dissertation.] Thesis, Wageningen.
    3. Castagnone-Sereno P., Kermarrec A. (1991): Invasion of tomato roots and reproduction of Meloidogyne incognita as an affected by raw sewage sludge. J. Nematol., 23: 724-728. Go to PubMed...
    4. Cook R. (1991): Resistance in plants to cyst and root-knot nematodes. Agr. Zool. Rev., 4: 213-240.
    5. Fassuliotis G., Connick W.J., Jr. (1989): Encapsulation of Meloidogyne inciognita eggs in carrageenan. J. Nematol., 21: 540-543. Go to PubMed...
    6. Fazal M., Shah N.H., Imran M., Siddiqui Z.A. (1994): Responses of some tomato cultivars to root-knot nematode Meloidogyne incognita. Tests Agrochem. Cult., 15: 126-127.
    7. Fortnum B.A., Kasperbauer M.J., Hunt P.G., Bridges W.C. (1991): Biomass partitioning in tomato plants infected with Meloidogyne incognita. J. Nematol., 23: 291-297. Go to PubMed...
    8. Huang J.S. (1983): Mechanisms of resistance to root-knot nematodes. Adv. Treatise Meloidogyne, 1: 165-174.
    9. Kaloshian I., Lange W.H., Williamson V.M. (1995): An aphid-resistance locus is tightly linked to the nematode-resistance gene, Mi, in tomato. Proc. Nat. Acad. Sci. USA, 92: 622-625. Go to original source... Go to PubMed...
    10. Tesařová B., Zouhar M., Ryšánek P. (2003): Development of PCR for specific determination of root-knot nematode Meloidogyne incognita. Plant Prot. Sci., 39: 23-28. Go to original source...
    11. Veremis J.C., Roberts P.A. (1996): Identification of resistance to Meloidogyne javanica in the Lycopersicon peruvianum complex. Theor. Appl. Genet., 93: 894-901. Go to original source... Go to PubMed...
    12. Vos P., Simons G., Jesse T., Wijbrandi J., Heinen L., Hogers R., Frijters A., Groenendijk J., Diergaarde P., Reijans M., Fierens-Onstenk J. (1998): The tomato Mi-1 gene confers resistance to both root-knot nematodes and potato aphids. Nat. Biotechnol., 16: 1365-1369. Go to original source... Go to PubMed...
    13. Williamson V.M., Ho J.Y., Wu F.F., Miller N., Kaloshian I. (1994): A PCR-based marker tightly linked to the nematode resistance gene, Mi in tomato. Theor. Appl. Genet., 87: 757-763. Go to original source... Go to PubMed...
    14. Wordragen M.F., Weide R. van, Liharska T., Steen A., Koornneef M. van der, Zabel P. (1994): Genetic and molecular organization of the short arm and pericentromeric region of tomato chromosome 6. Euphytica: Neth. J. Plant Breed., 79: 169-174. Go to original source...
    15. Yaghooobi J., Kaloshian I., Wen Y., Williamson V.M. (1995): Mapping a new nematode resistance locus in Lycopersion peruvianum. Theor. Appl. Genet., 91: 457-464. Go to original source... Go to PubMed...

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