Plant Soil Environ., 2004, 50(2):70-74 | DOI: 10.17221/3683-PSE

Study of European and Czech populations of potato cyst nematodes (Globodera rostochiensis and G. pallida) by RAPD method

P. Sedlák, M. Melounová, S. Skupinová, P. Vejl, J. Domkářová
1 Czech University of Agriculture in Prague, Czech Republic
2 Potato Research Institute, Ltd., Havlíčkův Brod, Czech Republic

Potato cyst nematodes (PCN) are the big problem in worldwide planting of potatoes and another Solanaceous plants. Identification of individual pathotypes according to international scheme is very demanding but a very important part of the phytosanitary process to control these pests. Molecular genetic identification of different plant and animal species or individuals is a very interesting way at the present time and let's hope that it will be important in future. This report presents results of the RAPD study of nine different real PCN populations. There were five Globodera rostochiensis populations and four G. pallida populations. Pathotypes Ro2, Ro2/3, Ro4, Ro5, Pa2 and Pa3 were from European populations; population Ro1 and X were of Czech provenance. Genetics variable of these populations was described by a set of six decameric primers (OPA 07, OPG 03, OPG 05, OPG 08, OPG 10 and OPG 13). Genetic dissimilarity was by Gel Manager for Windows evaluated. Detectable differences behind all populations were found and the dendrogram was compiled. The unknown population X was sorted into group of Globodera pallida species subgroup of Pa2 consequently.

Keywords: potato cyst nematodes; Globodera rostochiensis; G. pallida; pathotypes; RAPD

Published: February 29, 2004  Show citation

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Sedlák P, Melounová M, Skupinová S, Vejl P, Domkářová J. Study of European and Czech populations of potato cyst nematodes (Globodera rostochiensis and G. pallida) by RAPD method. Plant Soil Environ. 2004;50(2):70-74. doi: 10.17221/3683-PSE.
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