Plant Soil Environ., 2006, 52(11):485-491 | DOI: 10.17221/3538-PSE

Evaluation of spring barley genotypes with different susceptibility to Fusarium head blight using molecular markers

Z. Nesvadba1,2, T. Vyhnánek3, I. Ježíšková3, L. Tvarůžek1,2, M. Špunarová1,2, J. Špunar1,2
1 AgriculturalResearch Institute Kroměříž, Ltd., Czech Republic
2 Agrotest fyto, Ltd., Kroměříž, Czech Republic
3 Mendel University of Agriculture and Forestry in Brno, Czech Republic

One of cereal diseases that has gained a greater importance for growers, processing industry as well as for breeders in many regions of the world is Fusarium head blight (FHB) caused by fungi of the Fusarium genus. The objectives of this study were to test diversity among spring barley breeding lines exhibiting various sensitivity to FHB and to find RAPD markers and AFLP markers that will distinguish between susceptible and resistant and/or moderately resistant genotypes. A test of a set of spring barley genotypes artificially infected by fusaria in field trials was carried out. Based on the results from field and laboratory evaluation and deoxynivalenol (DON) content assessment, barley genotypes with different responses to FHB were selected. The genotypes were hybridized and doubled haploid (DH) lines were derived in F1 generation using the in vitro androgenesis method. Initial parental components and derived DH lines were tested for FHB infection and DON content. A set of parental genotypes of spring barley was tested with 80 RAPD markers. A RAPD marker (H30) was detected which enabled to distinguish between very susceptible parental genotypes and other resistant or moderately resistant spring barley genotypes based on the fragment of about 1300 bp. This specific product was screened in 23 DH lines derived from crosses of parental genotypes of spring barley and detected in 10 DH lines. During the study, some DH lines were selected that exhibited improved resistance to Fusarium infection. A low infection level and low DON content was found in the line DH 4/2 derived from CI 4196 × Foster. The AFLP technique was used to analyse parental genotypes of spring barley. The detected markers can be further evaluated and employed to select breeding materials.

Keywords: spring barley; Fusarium head blight; deoxynivalenol; doubled haploid lines; RAPD; AFLP

Published: November 30, 2006  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Nesvadba Z, Vyhnánek T, Ježíšková I, Tvarůžek L, Špunarová M, Špunar J. Evaluation of spring barley genotypes with different susceptibility to Fusarium head blight using molecular markers. Plant Soil Environ. 2006;52(11):485-491. doi: 10.17221/3538-PSE.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Agrama H.A., Dahleen L.S., McArthur R.I. (2004): Cloning of AFLP fragments linked to resistance genes for Fusarium head blight in barley. In: Proc. 9th Int. Barley Genet. Symp. Brno: 760-762.
    2. Armstrong K., Ho K.M., Fedak G., Martin R., Butler G., Savard M., Xue A., Langille L., Sabo F., Kuc M., Burville M. (2001): A study to identify molecular markers for Fusarium head blight in six-row barley. In: Proc. 2 nd Can. Worksh. Fusarium head blight: 22-25.
    3. Buerstmayr H., Legzdina L., Steiner B., Lemmens M. (2004): Variation for resistance to FHB in spring barley. In: Proc. 9 th Int. Barley Genet. Symp. Brno: 308-315.
    4. Hou L., Ullrich S.E., Kleinhofs A. (1994): Inheritance of anther culture traits in barley. Crop Sci., 34: 1243-1247. Go to original source...
    5. Kolb F.L., Bai G.H., Muehlbauer G.J., Anderson J.A., Smith K.P., Fedak G. (2001): Host plant resistance genes for Fusarium head blight: Mapping and manipulation with molecular markers, Crop. Sci., 41: 611-619. Go to original source...
    6. Machii H., Mizuno H., Hirabayashi T., Li H., Hagio T. (1998): Screening wheat genotypes for high callus induction and regeneration capability from anther and immature embryo cultures. Plant Cell Tiss. Org. Cult., 53: 67-74. Go to original source...
    7. Mueller U.G., Wolfenbarger L.L. (1999): AFLP genotyping and fingerprinting. Trends Ecol. Evol., 14: 389-394. Go to original source... Go to PubMed...
    8. Nei M., Li W.H. (1979): Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Nat. Acad. Sci. USA, 76: 5269-5273. Go to original source... Go to PubMed...
    9. Ovesná J., Poláková K., Leišová L. (2002): DNA analyses and their applications in plant breeding. Czech J. Genet. Plant Breed., 38: 29-40. Go to original source...
    10. Saghai-Maroof M.A., Soliman K.M., Jorgensen R.A., Allard R.W. (1984): Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location and population dynamics. Proc. Nat. Acad. Sci. USA, 81: 8014-8018. Go to original source... Go to PubMed...
    11. Schaafsma A.W., Savard M.E., Clear R., Dexter J. (2004): Methods and issues regarding detection of deoxynivalenol, Fusarium - damaged kernels, and Fusarium spp. in commercial grain in Canada. Can. J. Plant. Pathol., 26: 443-452. Go to original source...
    12. Schwarz P.B. (2003): Impact of Fusarium head blight on malting and brewing quality of barley. In: Leonard K.J., Bushnell W.R. (eds.): Fusarium Head Blight of Wheat and Barley. APS Press, St. Paul, Minnesota, USA: 395-419.
    13. Špunarová M., Ovesná J., Tvarůžek L., Kučera L., Špunar J., Hollerová I. (2005): The use of molecular markers for characterisation of spring barley for breeding to Fusarium head blight. Plant Soil Environ., 51: 483-490. Go to original source...
    14. Steffenson B.J. (2003): Fusarium head blight of barley. In: Leonard K.J., Bushnell W.R. (eds.): Fusarium Head Blight of Wheat and Barley. APS Press, St. Paul, Minnesota, USA: 241-295.
    15. Takeda K. (2004): Inheritance of the Fusarium head blight resistance in barley. In: Proc. 9 th Int. Barley Genet. Symp. Brno: 302-307.
    16. Takeda K., Heta H. (1989): Establishing the testing method and a search for the resistant varieties to Fusarium head blight in barley. Japan. J. Breed., 39: 203-216. Go to original source...
    17. Tvarůžek L., Ji L., Cao K. (2003): Reaction of winter wheat genotypes from Chinese and Czech collection to Fusarium head blight and leaf diseases. In: Dagun L. (ed.): Research of Plant Pathology in Hebei. China Agriculture Press, 1: 94-100.
    18. Vagera J., Ohnoutková L. (1993): In vitro production of androgenesis in wheat and barley. Rostl. Výr., 39: 97-114.
    19. Windels C.E. (2000): Economic and social impacts of Fusarium head blight: Changing farms and rural communities in the northern great plains. Phytopathology, 90: 17-21. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content