Plant Soil Environ., 2007, 53(7):317-320 | DOI: 10.17221/2211-PSE

Renaturation of telomere-binding proteins after the fractionation by SDS-polyacrylamide gel electrophoresis

G. Rotková
Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic

A simple method for identification and characterization of telomere-binding proteins is described in this article. After Sodium Dodecyl Sulphate-Polyacrylamide gel electrophoresis (SDS-PAGE), proteins are eluted, renatured and used for retardation analysis with labelled oligonucleotides corresponding to human and plant of telomeric sequences. We show here that this method is efficient to recover sequence-specific DNA-binding abilities of putative telomere-binding proteins.

Keywords: method; renaturation; telomere-binding proteins; telomeres

Published: July 31, 2007  Show citation

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Rotková G. Renaturation of telomere-binding proteins after the fractionation by SDS-polyacrylamide gel electrophoresis. Plant Soil Environ. 2007;53(7):317-320. doi: 10.17221/2211-PSE.
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