Plant, Soil and Environment, 2007 (vol. 53), issue 7
Mycorrhizal infection ameliorates chlorophyll content and nutrient uptake of lettuce exposed to saline irrigation
P. Zuccarini
Plant Soil Environ., 2007, 53(7):283-289 | DOI: 10.17221/2209-PSE 
Salinity is one of the most serious environmental problems influencing crop growth. Today, the use of microorganisms as biofertilizers in agriculture is quite diffused, and good results have been obtained in terms of induction of resistance to biotic and abiotic stresses in crops. The effects of inoculation with a mixture of the mycorrhizal fungi Glomus mosseae, G. intraradices and G. coronatum have been investigated on lettuce (Lactuca sativa L.) cultivated at three different levels of salinity of the irrigation water (0, 1.5 and 3 g NaCl/l) and collected during three subsequent samplings. Dry mass production was significantly...
Step-by-step morpho-physiological responses of Arachis hypogaea L. cv. NC 2 to iron deficiency
A. Gholizadeh, B. Baghban Kohnehrouz, H. Hekmatshoar
Plant Soil Environ., 2007, 53(7):290-298 | DOI: 10.17221/2212-PSE
Well-aerated and alkaline soils are proven to lack plant-available iron. Fe-efficient plants, however, induce morpho-physiological and biochemical mechanisms of adaptation. These changes in morphology and physiology of the shoot-root systems of peanut (Arachis hypogaea L. cv. NC 2) plants were studied by cultivating them hydroponically in the nutrient solution containing different levels of Fe3+EDDHA (0.00, 0.125, 0.25, 0.50, 1 and 2 ppm). Three types of chlorosis and regreening (I, II, III) on the shoot system appeared simultaneously with three types of rooting (I, II, III) in different stages of plant growth under Fe-free media....
Effect of perforated foil and polypropylene fibre covers on assimilation leaf area of early potato cultivars
W. Wadas, E. Kosterna
Plant Soil Environ., 2007, 53(7):299-305 | DOI: 10.17221/2208-PSE
This paper presents the results of a three-year research on the effect of perforated polyethylene foil and polypropylene fibre covering at various lengths of plant cover period (2 and 3 weeks after plant emergence) on the assimilation leaf area of early potato cultivars. In the case of plant covering for 2 weeks after emergence the assimilation leaf area and leaf area index (LAI) at the moment of cover removal were on average 2 times higher than in the cultivation without covering; at the 3-week period of plant covering they were almost 1.7 times higher. A higher favourable effect of covering was obtained in the year with the lowest air temperature...
Agricultural drought and spring barley yields in the Czech Republic
M. Trnka, P. Hlavinka, D. Semerádová, M. Dubrovský, Z. ®alud, M. Moľný
Plant Soil Environ., 2007, 53(7):306-316 | DOI: 10.17221/2210-PSE
The relationship between detrended district yields of spring barley (1961-2000) and meteorological drought was assessed by Palmer Z-index. We found that the seasonal water balance (April-June) significantly (P = 0.05) influences the spring barley production in 51 out of 62 evaluated districts. Coefficients of correlation varied in individual districts from 0.19 to 0.70, with the highest values being found in southern Moravia. Data analysis revealed the presence of six distinct groups of districts with a specific drought-yield relationship. The most drought-sensitive cluster included five districts in the South East of the country. On the other...
Renaturation of telomere-binding proteins after the fractionation by SDS-polyacrylamide gel electrophoresis
G. Rotková
Plant Soil Environ., 2007, 53(7):317-320 | DOI: 10.17221/2211-PSE
A simple method for identification and characterization of telomere-binding proteins is described in this article. After Sodium Dodecyl Sulphate-Polyacrylamide gel electrophoresis (SDS-PAGE), proteins are eluted, renatured and used for retardation analysis with labelled oligonucleotides corresponding to human and plant of telomeric sequences. We show here that this method is efficient to recover sequence-specific DNA-binding abilities of putative telomere-binding proteins.
Mapping of non-recombining regions via molecular markers
B. Janouąek, J. ®lůvová
Plant Soil Environ., 2007, 53(7):321-324 | DOI: 10.17221/2207-PSE
The lack of recombination in some genomic regions represents a serious obstacle in mapping studies. In this review, we describe methods that are currently used to overcome this problem. Main attention is given to the comparison of methods that are based on the principle of radiation hybrid mapping. We also discuss a strategy called HAPPY mapping (mapping based on the analysis of approximately HAPloid DNA samples using the PolYmerase chain reaction). In addition to reviewing the methods used by other authors, we also summarize our experience with deletion mapping of Y-chromosome in dioecious model plant species (Silene latifolia).
Chromosomal rearrangements in Arabidopsis mutants revealed by repeated FISH
P. Mokroą
Plant Soil Environ., 2007, 53(7):325-328 | DOI: 10.17221/2206-PSE
The stability of plant nuclear genome is a necessary condition for the faithful transmission of genetic information through cell lineages. When DNA damage occurs due to various impairments, cells start a number of repair processes including ligation of broken chromosomes. As a result, dicentric chromosomes can be formed. Dicentrics are easily detectable as anaphase bridges during following mitosis. Using Arabidopsis as a model plant, we developed a sensitive cytogenetic assay to identify specific chromosomal rearrangements. Here we show Arabidopsis tert-/- and atm-/- mutants and their chromosome rearrangements...