Plant Soil Environ., 2007, 53(7):325-328 | DOI: 10.17221/2206-PSE

Chromosomal rearrangements in Arabidopsis mutants revealed by repeated FISH

P. Mokro¹
Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic

The stability of plant nuclear genome is a necessary condition for the faithful transmission of genetic information through cell lineages. When DNA damage occurs due to various impairments, cells start a number of repair processes including ligation of broken chromosomes. As a result, dicentric chromosomes can be formed. Dicentrics are easily detectable as anaphase bridges during following mitosis. Using Arabidopsis as a model plant, we developed a sensitive cytogenetic assay to identify specific chromosomal rearrangements. Here we show Arabidopsis tert-/- and atm-/- mutants and their chromosome rearrangements and fusions analysed by fluorescence in situ hybridization (FISH). The method is based on successive rounds of FISH with chromosome-specific probes and the comparison of resulting FISH images.

Keywords: Arabidopsis; FISH; chromosomal fusions; BAC probes; rDNA loci

Published: July 31, 2007  Show citation

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Mokro¹ P. Chromosomal rearrangements in Arabidopsis mutants revealed by repeated FISH. Plant Soil Environ. 2007;53(7):325-328. doi: 10.17221/2206-PSE.
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