Plant Soil Environ., 2007, 53(8):350-354 | DOI: 10.17221/2213-PSE

An influence of cisplatin on the cell culture of Nicotiana tabacum BY-2

P. Babula1, V. ©upálková3,4, V. Adam2, L. Havel4, M. Beklová4, Z. Sladký1, R. Kizek3
1 Department of Natural Drugs, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic
2 Department of Veterinary Ecology and Environmental Protection, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic
3 Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry in Brno, Czech Republic
4 Department of Plant Biology, Mendel University of Agriculture and Forestry in Brno, Czech Republic

The mechanism of cisplatin effect has been well examined in in vitro models of malignant cell lines, but has never been studied on plant cell lines. When plant cell suspension culture of Nicotiana tabacum BY-2 was treated with cisplatin a decrease of viability with its increasing concentration was observed as well as the mitotic index. The structure of nuclei has also been changed in dependence on cisplatin concentration.

Keywords: cisplatin; Nicotiana tabacum BY-2; cell viability; nucleus; DNA

Published: August 31, 2007  Show citation

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Babula P, ©upálková V, Adam V, Havel L, Beklová M, Sladký Z, Kizek R. An influence of cisplatin on the cell culture of Nicotiana tabacum BY-2. Plant Soil Environ. 2007;53(8):350-354. doi: 10.17221/2213-PSE.
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