Plant, Soil and Environment, 2007 (vol. 53), issue 8
Interspecific variation and impact of clear-cutting on natural 15N abundance and N concentration in the needle-to-soil continuum of a boreal conifer forest
S.P. Sah, H. Ilvesniemi
Plant Soil Environ., 2007, 53(8):329-339 | DOI: 10.17221/2186-PSE 
The primary objective of this paper is to study the interspecific variation of 15N natural abundance in two dominant conifer tree species, Scot pine (Pinus sylvestris L.) and Norway spruce (Picea abies L. Karst) in a N-limited mixed boreal coniferous forest in southern Finland. Both a mature tree stand (a natural forest stand) and a stand of seedlings in a clear-cut area were considered. We analyzed %N and δ15N natural abundance in needles, branches, litter and soil of the both above-mentioned stands. Pine needles had higher N concentrations than spruce needles, both in mature forest (pine = 1.09%, SE ± 0.02 and...
Glutathione and zeaxanthin formation during high light stress in foliose lichens
J. Štepigová, H. Vráblíková, J. Lang, K. Večeřová, M. Barták
Plant Soil Environ., 2007, 53(8):340-344 | DOI: 10.17221/2187-PSE
In the presented study, we describe techniques for glutathione and pigment determination in lichens used in our laboratory. Glutathione and xanthophyll cycle pigments, especially zeaxanthin, are important antioxidants protecting plants against various stresses. In our laboratory, the high light stress in lichens has been intensively studied for several years. We extract glutathione in HCl and determine it by thiol-binding fluorescence label monobromobimane. For pigment determination, homogenized lichen thalli are extracted with pure acetone. According to our results, the total amount of glutathione decreases after a short-term high light exposure,...
The determination of avidin in genetically modified maize by voltammetric techniques
J. Petrlová, S. Křížková, V. Šupálková, M. Masařík, V. Adam, L. Havel, K.J. Kramer, R. Kizek
Plant Soil Environ., 2007, 53(8):345-349 | DOI: 10.17221/2185-PSE
Quality assurance is a major issue in the food industry. The authenticity of food ingredients and their traceability are required by consumers and authorities. Plant species such as barley (Hordeum vulgare), rice (Oryza sativa), sunflower (Helianthus annus), wheat (Triticum aestivum) and maize (Zea mays) are very common objects of interest of genetic modification (GMO); therefore the development of specific assays for their specific detection and quantification of GMO are needed. Furthermore, the production and trade of genetically modified lines from an increasing number of plant species brings about the need for...
An influence of cisplatin on the cell culture of Nicotiana tabacum BY-2
P. Babula, V. Šupálková, V. Adam, L. Havel, M. Beklová, Z. Sladký, R. Kizek
Plant Soil Environ., 2007, 53(8):350-354 | DOI: 10.17221/2213-PSE
The mechanism of cisplatin effect has been well examined in in vitro models of malignant cell lines, but has never been studied on plant cell lines. When plant cell suspension culture of Nicotiana tabacum BY-2 was treated with cisplatin a decrease of viability with its increasing concentration was observed as well as the mitotic index. The structure of nuclei has also been changed in dependence on cisplatin concentration.
Effect of selected factors on total carotenoid content in potato tubers (Solanum tuberosum L.)
Z. Kotíková, A. Hejtmánková, J. Lachman, K. Hamouz, E. Trnková, P. Dvořák
Plant Soil Environ., 2007, 53(8):355-360 | DOI: 10.17221/2214-PSE
In the years 2004 and 2005 precise field trials were performed with the aim to evaluate the effect of variety, year of cultivation, N, P, K and Mg fertilization, and the cover with white propylene fibre Paegas-Agro UV 17 on the total carotenoid content (TC) in potato tubers. TC was determined by absorption UV-VIS spectrophotometry and obtained results were statistically evaluated (ANOVA and MANOVA variance analyses, Scheffe's test). Statistically significant differences (P = 0.05) in the ability of varieties to synthesize carotenoids were found (Karin, Agria and Marabel with high TC × Impala and Saturna with low TC) and also the year of cultivation...
Heterologous approach in the search for (candidate) genes
J. Žlůvová
Plant Soil Environ., 2007, 53(8):361-364 | DOI: 10.17221/2216-PSE
To answer many questions of evolutionary developmental biology, it is necessary to identify genes which could be responsible for the studied traits. In this review, I have summarised the methods that are currently used to identify these genes. Mainly the PCR-based methods of gene cloning are discussed here, with the special attention given to the comparison of primer design strategies and PCR amplification strategies. In addition to the review of methods used by other authors, an overview of our experience with PCR-based gene cloning in dioecious model plant species Silene latifolia is included.
Approach to the assessment of transport risk of inorganic pollutants based on the immobilisation capability of soil
J. Makovníková, G. Barančíková, B. Pálka
Plant Soil Environ., 2007, 53(8):365-373 | DOI: 10.17221/2215-PSE
The objective of this paper is to elaborate a system of evaluation of potential risks of transport of inorganic elements, and to create a map of soil potential to immobilisation/transportation of potential risk elements. The categorization of these risks was realized in two layers, contamination and potential soil sorption. The level of contamination was evaluated according to the Slovak Soil Law. Potential sorption of soil (PSS) is formed by qualitative (soil reaction, pH value, optical value of soil humus), and quantitative factors (C