Plant Soil Environ., 2009, 55(7):305-310 | DOI: 10.17221/62/2009-PSE

Isolation and characterization of ardicrenin from Ardisia crenata Sims

Yuan Ma1, Shangrao Pu2, Qingsu Cheng3, Mingdong Ma2
1 Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu, P.R. China
2 Department of Resource and Environment, Sichuan Agriculture University, Dujiangyan, P.R. China
3 Department of Pharmacy and Bioengineering, Chemical School, Sichuan University, Chengdu, P.R. China

A new, effective and economical method to extract ardicrenin from Ardisia crenata Sims collected in the Wolong natural reserve, Sichuan, China, is established. Ardisia crenata Sims powder is counter-current extracted with 80% methanol reflux, decompressively enriched and centrifuged to defat. Supernatant is applied to macroporous resin column (AB-8) with 80% methanol, ardicrenin is isolated by silica gel chromatography with dichlormethane-acetoacetate-methanol (4:1.5:1) washing, and recrystallized in methanol. The final product which proved to be ardicrenin by analytic procedure including Furier transform infrared (FTIR) and ultraviolet spectrum (UV), mass spectroscopy (MS), nuclear magnetic resonance (NMR) and high performance liquid chromatography (HPLC) is white amorphous powder with yield of 1.59 ± 0.02%.

Keywords: Ardisia crenata Sims; ardicrenin; isolation; determination

Published: July 31, 2009  Show citation

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Ma Y, Pu S, Cheng Q, Ma M. Isolation and characterization of ardicrenin from Ardisia crenata Sims. Plant Soil Environ. 2009;55(7):305-310. doi: 10.17221/62/2009-PSE.
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