Plant Soil Environ., 2010, 56(10):463-469 | DOI: 10.17221/149/2009-PSE

The effect of benzothiazolium salt on spruce callus cells

Ľ. Krajnáková1,2, D. Kákoniová1, D. Lišková1, E. Hlinková3
1 Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia
2 NovaMed Policlinic, Banská Bystrica, Slovakia
3 Institute of Cell Biology, Faculty of Natural Sciences, Comenius University,

The effect of 3-(benzyloxycarbonylmethyl)-2-benzothiazolinone (SM-550) on the growth parameters, cell division, and cell polymorphism of spruce callus cells (Picea abies L. Karst) was investigated. These results were compared with callus parameters grown on the medium supplemented with NAA. The highest concentration (1 mmol) of SM-550 stimulated the growth process, as well as cell division, shortened the lag-phase, and had a significant effect on cells polymorphism. Its effect was demonstrated especially on long-term culture (3 subcultures - 84 days). On the other hand, the highest stimulation of growth by SM-550 in 1 μmol concentration was determined only in the first subculture. SM-550 in the lowest concentration (1 nmol) used was completely unsuitable in the third subculture, the callus was necrotic and resembled to calli growing on the medium without growth hormones.

Keywords: benzothiazolium salt; callus culture; linear cell polymorphism; Picea abies L. Karst

Published: October 31, 2010  Show citation

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Krajnáková Ľ, Kákoniová D, Lišková D, Hlinková E. The effect of benzothiazolium salt on spruce callus cells. Plant Soil Environ. 2010;56(10):463-469. doi: 10.17221/149/2009-PSE.
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