Plant Soil Environ., 2011, 57(1):7-10 | DOI: 10.17221/116/2010-PSE

Impact of tryptophan and glutamine on the tissue culture of upland rice

E. Shahsavari
School of Biological Sciences, Flinders University, Adelaide, Australia

In order to evaluate the effect of tryptophan and glutamine on the tissue culture of upland rice cultivars, serial experiments were conducted using four cultivars: Kusan, Lamsan, Selasi and Siam. Mature seeds from these cultivars were subjected to 4 levels of tryptophan and glutamine on the MSB5 (MS macro elements, B5 micro elements and B5 vitamins) medium. Callus induction results showed a positive effect of tryptophan on all cultivars except Selasi. The optimal tryptophan concentration for callus induction in cultivars Kusan and Siam was 100 µmol, while in Lamsan the optimum was 200 µmol. With the exception of the Lamsan cultivar, incorporation of glutamine generally did not result in the enhancement of callus induction response that incorporation of tryptophan did. Plantlet regeneration frequency was significantly increased when an appropriate level of tryptophan was added to culture media, the optimum being 100 µmol or Kusan, Selasi and Siam, compared to an optimum of 200 µmol for Lamsan. Glutamine did not affect regeneration frequency in any of the cultivars under the conditions tested. In summary, the results showed that tryptophan is a useful additive for upland rice tissue culture.

Keywords: callus induction frequency; plantlet regeneration frequency; embryogenic calli

Published: January 31, 2011  Show citation

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Shahsavari E. Impact of tryptophan and glutamine on the tissue culture of upland rice. Plant Soil Environ. 2011;57(1):7-10. doi: 10.17221/116/2010-PSE.
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    References

    1. Bajaj S., Mohanty A. (2005): Recent advances in rice biotechnology-towards genetically superior transgenic rice. Plant Biotechnology Journal, 3: 275-307. Go to original source... Go to PubMed...
    2. Bernier J., Atlin G.N., Serraj R., Kumar A., Spaner D. (2008): Breeding upland rice for drought resistance. Journal of the Science of Food and Agriculture, 88: 927-939. Go to original source...
    3. Chowdhry C.N., Tyagi A.K., Maheshwari N., Maheshwari S.C. (1993): Effect of l-proline and l-tryptophan on somatic embryogenesis and plantlet regeneration of rice (Oryza sativa L. cv. Pusa 169). Plant Cell, Tissue and Organ Culture, 32: 357-361. Go to original source...
    4. Compton M.E. (1994): Statistical methods suitable for the analysis of plant tissue culture data. Plant Cell, Tissue and Organ Culture, 37: 217-242. Go to original source...
    5. Dabul A., Belefant-Miller H., RoyChowdhury M., Hubstenberger J., Lorence A., Phillips G. (2009): Screening of a broad range of rice (Oryza sativa L.) germplasm for in vitro rapid plant regeneration and development of an early prediction system. In Vitro Cellular and Developmental Biology - Plant, 45: 414-420. Go to original source... Go to PubMed...
    6. Gamborg O.L., Miller R.A., Ojima K. (1968): Nutrient requirements of suspension cultures of soybean root cells. Experimental Cell Research, 50: 151-158. Go to original source... Go to PubMed...
    7. Ge X., Chu Z., Lin Y., Wang S. (2006): A tissue culture system for different germplasms of indica rice. Plant Cell Reports, 25: 392-402. Go to original source... Go to PubMed...
    8. Geng P.P., La H.G., Wang H.Q., Stevens E.J.C. (2008): Effect of sorbitol concentration on regeneration of embryogenic calli in upland rice varieties (Oryza sativa L.). Plant Cell, Tissue and Organ Culture, 92: 303-313. Go to original source...
    9. George E.F., Hall M.A., Klerk G.-J.D. (2008): Plant Propagation by Tissue Culture. Volume 1. The Background, Springer, Dordrecht. Go to original source...
    10. Koetje D.S., Grimes H.D., Yi-Chang W., Hodges T.K. (1989): Regeneration of indica rice (Oryza sativa L.) from primary callus derived from immature embryos. Journal of Plant Physiology, 135: 184-190. Go to original source...
    11. Murashige T., Skoog F. (1962): A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiology Plantarum, 15: 473-497. Go to original source...
    12. Siriwardana S., Nabors M.W. (1983): Tryptophan enhancement of somatic embryogenesis in rice. Plant Physiology, 73: 142-146. Go to original source... Go to PubMed...
    13. Shahsavari E., Maheran A.A., Siti Nor Akmar A., Hanafi M.M. (2010): The effect of plant growth regulators on optimization of tissue culture system in Malaysian upland rice. African Journal of Biotechnology, 9: 2089-2094.
    14. Tyagi A.K., Mohanty A. (2000): Rice transformation for crop improvement and functional genomics. Plant Science, 158: 1-18. Go to original source... Go to PubMed...
    15. Zaidi M.A., Narayanan M., Sardana R., Taga I., Postel S., Johns R., McNulty M., Mottiar Y., Mao J., Loit E., Altosaar I. (2006): Optimizing tissue culture media for efficient transformation of different indica rice genotypes. Agronomy Research, 4: 563-575.

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