Plant Soil Environ., 2012, 58(5):205-210 | DOI: 10.17221/432/2011-PSE

Impact of beauvericin on membrane properties of young initial leaves of maize with different susceptibility to Fusarium

J. Pavlovkin1, I. Mistríková2, K. Jašková1, L. Tamás1
1 Institute of Botany, Slovak Academy of Sciences, Bratislava, Slovakia
2 Department of Pharmacognosy and Botany, Faculty of Pharmacy, Comenius University, Bratislava, Slovakia

In the present study the impact of beauvericin (BEA) on the cell membrane properties and respiration of young initial leaves of maize were studied using two maize cultivars differing in their susceptibility to Fusarium sp. BEA significantly depolarized EM of leaf parenchymal cells and this depolarization showed time and dose dependency regardless on the sensitivity of maize cultivars to Fusarium. However, the extent of BEA-induced depolarization was 2-5 times higher in sensitive cv. Pavla than in tolerant cv. Lucia. Membrane permeability and K+ leakage from leaves cells treated with BEA was higher in sensitive cv. Pavla but the differences were not so considerable than the depolarization of EM. Treatment of maize young initial leaves with 40 μmol BEA significantly inhibited respiration. In accord with electrophysiological measurements inhibition of respiration was higher in sensitive cv. Pavla showing 70% inhibition already after 90 min of BEA treatment while in tolerant cv. Lucia inhibition represented only 27%. The biological activity of BEA seems to be mediated by the ability of BEA to affect membrane permeability and ion transport. This is probably the initial effect of BEA on plant cell leading to subsequent effect on other cell organelles (mitochondria) and cell metabolism.

Keywords: beauvericin; membrane potential; membrane permeability; respiration; phytotoxicity; young maize leaves; Zea mays L.

Published: May 31, 2012  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Pavlovkin J, Mistríková I, Jašková K, Tamás L. Impact of beauvericin on membrane properties of young initial leaves of maize with different susceptibility to Fusarium. Plant Soil Environ. 2012;58(5):205-210. doi: 10.17221/432/2011-PSE.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Atkin O.K., Edwards E.J., Loveys B.R. (2000): Response of root respiration to changes in temperature and its relevance to global warming. New Phytologist, 147: 141-154. Go to original source...
    2. Chen B.F., Tsai M.C., Jow G.M. (2006): Induction of calcium influx from extracellular fluid by beauvericin in human leukemia cells. Biochemical and Biophysical Research Communication, 340: 134-139. Go to original source... Go to PubMed...
    3. Hamil R.I., Higgens C.E., Boaz H.E., Gorman M. (1969): The structure of beauvericin, a new depsipeptide antibiotic toxic to Artemia salina. Tetrahedron Letters, 49: 4255-4258. Go to original source...
    4. Lemmens M., Joseph R., Schuhmacher R., Grausgruber H., Buerstmayr H., Ruckenbauer P., Neuhold G., Fidesser M., Krska R. (1997): Head blight (Fusarium spp.) on wheat: investigation on the relationship between disease symptoms and mycotoxin content. Cereal Research Communication, 25: 459-465. Go to original source...
    5. Logrieco A., Moretti A., Fornelli F., Fogliano V., Ritieni A., Caiaffa M., Randazzo G., Bottalico A., Macchia L. (1996): Fusaproliferin production by Fusarium subglutinans and its toxicity to Artemia salina, SF-9 insect cells, and IARC/LCL 171 human B-lymphocytes. Applied and Environmental Microbiology, 62: 3378-3784. Go to original source... Go to PubMed...
    6. Logrieco A., Moretti A., Ritieni A., Caiaffa M.F., Macchia L. (2002): Beauvericin: chemistry, biology and significance. In: Upadhyay R. (ed.): Advances in Microbial Toxin Research and its Biotechnological Exploitation. Kluwer Academic/Plenum Publisher, London, 23-30. Go to original source...
    7. Macchia L., Caiaffa M.F., Fornelli F., Calō L., Nenna S., Moretti A., Logrieco A., Tursi A. (2002): Apoptosis induced by the Fusarium mycotoxin beauvericin in mammalian cells. Journal of Applied Genetics, 434: 363-371.
    8. Marrè E. (1979): Fusicoccin: a tool in plant physiology. Annual Review of Plant Physiology, 30: 273-288. Go to original source...
    9. Moretti A., Logrieco A., Bottalico A., Ritieni A., Randazzo G., Corda P. (1995): Beauvericin production by Fusarium subglutinans from different geographical areas. Mycological Research, 99: 282-286. Go to original source...
    10. Neuhold G., Fidesser M., Krska R. (1977): Head blight (Fusarium spp.) on wheat: investigation on the relationship between disease symptoms and mycotoxin content. Cereal Research Communication, 25: 459-465. Go to original source...
    11. Paciolla C., Dipierro N., Mule G., Logrieco A., Dipierro S. (2004): The mycotoxins beauvericin and T-2 induce cell death and alteration to the ascorbate metabolism in tomato protoplasts. Physiological and Molecular Plant Physiology, 65: 49-56. Go to original source...
    12. Ojcius D.M., Zychlinsky A., Zheng L.M., Young J.E. (1991): Ionophore induced apoptosis: role of DNA fragmentation and calcium fluxes. Experimental Cell Research, 197: 43-49. Go to original source... Go to PubMed...
    13. Pavlovkin J., Mistrík I., Prokop M. (2004): Some aspects of the phytotoxic action of fusaric acid on primary Ricinus roots. Plant, Soil and Environment, 50: 397-401. Go to original source...
    14. Pavlovkin J., Mistríková I., Luxová M., Mistrík I. (2006): Effects of beauvericin on root cell transmembrane electric potential, electrolyte leakage and respiration of maize roots with different susceptibility to Fusarium. Plant, Soil and Environment, 52: 492-498. Go to original source...
    15. Pavlovkin J., Jašková K., Mistríková I., Tamás L. (2011): Impact of fusaproliferin on primary roots of maize cultivars differing in their susceptibility to Fusarium. Biologia, 66: 1044-1051. Go to original source...
    16. Polacco J.C., Sands D.C. (1977): The mycotoxin patulin inhibits respiration of higher plant cells. Plant Science Letters, 9: 121-128. Go to original source...
    17. Santini A., Šrobárová A., Pavlovkin J., Čiamporová M., Ritieni A. (2008): Fusaproliferin effects on the photosystem in the cells of maize seedling leaves. European Journal of Plant Pathology, 120: 363-371. Go to original source...
    18. Šrobárová A., Teixeira da Silva J.A., Kogan G., Ritieni A., Santini A. (2009): Beauvricin decrease cell viability of wheat. Chemistry and Biodiversity, 6: 1208-1215. Go to original source... Go to PubMed...
    19. Tomoda H., Huang X.-H., Cao J., Nishida H., Nagano R., Okuda S. (1992): Inhibition of acyl-CoA: cholesterol acyltransferase activity by cyclodepsipeptide antibiotics. Journal of Antibiotics, 45: 1626-1632. Go to original source... Go to PubMed...
    20. Vianello M., Macri F. (1978): Effect of zearalenone (F-2) on pea steam, maize root and rat liver mitochondria. Planta, 153: 443-446. Go to original source... Go to PubMed...
    21. Yao N., Tada Y., Sakamoto M., Nakayashiki H., Park P., Tosa Y., Mayama S. (2002): Mitochondrial oxidative burst involved in apoptotic response in oats. Plant Journal, 30: 567-579. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content